14 research outputs found

    Effector gene variation in Polish and Norwegian Phytophthora infestans strains

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    Poster presented at 2023 IS-MPMI Congress, Molecular Plant-Microbe Interactions, Providence, USA The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055

    Differences in Avr-vnt1 alleles and aggressiveness in four European Phytophthora infestans lineages

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    Poster presented at 12TH INTERNATIONAL CONGRESS OF PLANT PATHOLOGY ICPP2023, Lyon, France The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055

    Aggressiveness test of Phytophthora infestans isolates with different effector alleles

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    Poster presented at EAPR Pathology & Pests Section Meeting, Arras, France The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055

    Isolation, Identification and Preservation of Fusarium SPP. Causing Dry Rot of Potato Tubers

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    Fungi of the genus Fusarium cause dry rot, a potato disease which develops during long-term storage of tubers. The disease-inducing capabilities differ among Fusarium spp., but may also vary within species uni-versally considered main dry rot agents. Identification of Fusarium spp. present on diseased tubers in a surveyed area can help minimize crop losses and mycotoxin contamination by, for example, applying proper fungicides or planning crop rotation. Here, we present a procedure of obtaining single spore colonies of Fusarium spp. from potato tubers infected by dry rot, their identification using molecular methods and ways of preservation

    Phytophthora Infestans: Isolation of Pure Cultures, Storage and Inoculum Preparation

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    Phytophthora infestans causes potato and tomato late blight, economically the most important disease of these plant species. The Oomycete pathogen is frequently sampled, isolated to pure cultures, stored, and char-acterized. The knowledge of its diversity, migrations and evolution is essential for breeding resistant plants and for designing appropriate control strategies. The article presents methods for collection, storage and prep-aration of P. infestans isolates for inoculation of plant tissues, based on the publication by Zarzycka (2001), later updated and modified

    The role of Phytophthora infestans oospores in primary infection of potato foliage in Poland.

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    The study on oospore formation ability under natural conditions was carried out on the base of comparative characterization of four local populations of P. infestans. It was found that oospores can be formed in potato tissues under natural conditions. The comparison of proportion of both mating types isolates, high level of race complexity and racial diversity indicates, that oospores in experimental fields could play a role as a source of primary inoculum in three experimental localities in Boguchwała, in Przychojec and in Olesno Śląskie.The oospores were produced also in all combinations of pot and field experiments. They were formed most abundantly, when the potato plants were inoculated with mixed inoculum consisted of A1 and A2 spores, in 1:1 ratio and significantly less abundantly, when the inoculum was dominated by one of mating type isolate. The effect of time of subsequent inoculation with A1 or A2 isolates on oospore formation was observed: significantly more oospores were formed when the plants were inoculated with both mating types at the same time

    Effect of deep freezing of Phytophtora infestans on their survival and pathogenicity

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    The maintenance of Phytophthora infestans cultures isolates from blighted potato, frozen in liquid nitrogen, was studied in two experiments. The effects of deep freezing and acclimatization pre-treatment at different temperatures on culture survival and stability of virulence and aggressiveness were evaluated. The best survival of cultures maintained for three months in liquid nitrogen was expressed, when cultures were acclimatized before freezing at 7˚C. The survival of frozen cultures was significantly worse in comparison with control combinations stored on rye-agar and rye-agar under paraffin oil for short time (in experiment I – 40 days, in experiment II – three months). The virulence spectrum of frozen cultures after thawing was more narrow than that one observed before culture freezing. However this virulence spectrum did not differ significantly from virulence range of control cultures. The level of aggressiveness of culture stored in liquid nitrogen did not differ significantly from control cultures as well. The virulence spectrum and aggressiveness level of cultures frozen in liquid nitrogen were significantly higher after two passages of thawed cultures on potato tissues than after single passage
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