Additional file 2: of Expression patterns of STAT3, ERK and estrogen-receptor Îą are associated with development and histologic severity of hepatic steatosis: a retrospective study

Immunohistochemical staining for leptin (A-20, 1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and leptin-receptor (B-3, 1:25; Santa Cruz Biotechnology) in hepatic steatosis cases, and non-neoplastic hepatocytes and carcinoma cells from hepatocellular carcinomas. (a-b) Hepatic steatosis with nuclear and/or cytoplasmic staining of leptin (a), and diffuse granular staining of leptin-receptor (b). (c-e) Leptin in hepatocellular carcinomas. Nuclear and cytoplasmic staining of leptin in non-neoplastic hepatocytes (left side of each picture), and negative (c), weak (d) or strong (e) leptin staining in hepatocellular carcinoma cells (right side of each picture). (f-g) Leptin-receptor in hepatocellular carcinomas. Diffuse granular cytoplasmic expression of leptin-receptor in non-neoplastic hepatocytes (left), and weak focal (f) or strong diffuse (g) staining of leptin-receptor in hepatocellular carcinoma cells (right). (JPEG 4962 kb

Additional file 1: of Expression patterns of STAT3, ERK and estrogen-receptor Îą are associated with development and histologic severity of hepatic steatosis: a retrospective study

Differential features between non-alcoholic fatty liver disease (NAFLD) subgroups stratified by NAS and SAF. (DOCX 47 kb