1 research outputs found
Fast Characterization of Polyplexes by Taylor Dispersion Analysis
In a single procedure, Taylor dispersion
analysis (TDA) was used
for the size characterization of polyplexes and the quantification
of free polycation contained in excess within the polyplex sample.
TDA analysis was carried out in frontal mode for a better sensitivity
of detection. The proof of concept was established using a model polyplex
generated from the mixture of linear polylysine (DP 20) and DNA from
salmon testes at nitrogen to phosphate (N/P) ratio of 12. Polyplex
hydrodynamic radius was compared to the values obtained by dynamic
light scattering measurements. TDA was found to give access to the
weight-average hydrodynamic radius, while DLS basically gives an intensity-average
(harmonic <i>z</i>-average) value. The method was next applied
to the study of various polyplexes issued from polylysines of various
DP (50, 100) and different topologies (dendrigraft polylysines of
generation 2 and 3). This new methodology should greatly contribute
to the physicochemical characterization of polyplexes used for gene
transfection