High speed optical tomography for flow visualization

A novel optical architecture (based on holographic optical elements) for making high speed tomographic measurements is presented. The system is designed for making density or species concentration measurements in a nonsteady fluid or combustion flow. Performance evaluations of the optical system are discussed, and a test phase object was successfully reconstructed using this optical arrangement

Observable implications of Nash and subgame-perfect behavior in extensive games

We provide necessary and sufficient conditions for observed outcomes in extensive game forms, in which preferences are unobserved, to be rationalized first, partially, as a Nash equilibrium and then, fully, as the unique subgame-perfect equilibrium. Thus, one could use these conditions to find that play is (a) consistent with subgame-perfect equilibrium, or (b) not consistent with subgame-perfect equilibrium but is consistent with Nash equilibrium, or (c) consistent with neither.Revealed preference, subgame-perfect equilibrium

Stable Isotopes and Climate Change

The following description of the role of the study of stable isotopes in water and carbon dioxide molecules when constructing a record of Earth’s pattern of climate change is an excerpt from: Climate Change and Society by Raymond S. Bradley & Norman E. Law (2001) Nelson Thornes, Cheltenham, UK (ISBN: 0 7487 5823 2

City-level Geolocation of Tweets for Real-time Visual Analytics

Real-time tweets can provide useful information on evolving events and situations. Geotagged tweets are especially useful, as they indicate the location of origin and provide geographic context. However, only a small portion of tweets are geotagged, limiting their use for situational awareness. In this paper, we adapt, improve, and evaluate a state-of-the-art deep learning model for city-level geolocation prediction, and integrate it with a visual analytics system tailored for real-time situational awareness. We provide computational evaluations to demonstrate the superiority and utility of our geolocation prediction model within an interactive system.Comment: 4 pages, 2 tables, 1 figure, SIGSPATIAL GeoAI Worksho

Yaaka Hula Hickey Dula (Hawaiian Love Song) / words by E. Ray Goetz, Joe Young, and Pete Wendling

Cover: a photo of Al. Jolson; a drawing of a island; Publisher: Waterson Berlin and Snyder Co. (New York)https://egrove.olemiss.edu/sharris_c/1117/thumbnail.jp

Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues

Rapid detection of microbial-induced cellular changes during the course of an infection is critical to understanding pathogenesis and immunological homeostasis. In the last two decades, fluorescence imaging has received significant attention for its ability to help characterize microbial induced cellular and tissue changes in in vitro and in vivo settings. However, most of these methods rely on the covalent conjugation of large exogenous probes and detection methods based on intensity-based imaging. Here, we report a quantitative, intrinsic, label-free, and minimally invasive method based on two-photon fluorescence lifetime (FLT) imaging microscopy (2p-FLIM) for imaging 1,4-dihydro-nicotinamide adenine dinucleotide (NADH) metabolism of virally infected cells and tissue sections. To better understand virally induced cellular and tissue changes in metabolism we have used 2p-FLIM to study differences in NADH intensity and fluorescence lifetimes in HIV-1 infected cells and tissues. Differences in NADH fluorescence lifetimes are associated with cellular changes in metabolism and changes in cellular metabolism are associated with HIV-1 infection. NADH is a critical co-enzyme and redox regulator and an essential biomarker in the metabolic processes. Label-free 2p-FLIM application and detection of NADH fluorescence using viral infection systems are in their infancy. In this study, the application of the 2p-FLIM assay and quantitative analyses of HIV-1 infected cells and tissue sections reveal increased fluorescence lifetime and higher enzyme-bound NADH fraction suggesting oxidative phosphorylation (OxPhos) compared to uninfected cells and tissues. 2p-FLIM measurements improve signal to background, fluorescence specificity, provide spatial and temporal resolution of intracellular structures, and thus, are suitable for quantitative studies of cellular functions and tissue morphology. Furthermore, 2p-FLIM allows distinguishing free and bound populations of NADH by their different fluorescence lifetimes within single infected cells. Accordingly, NADH fluorescence measurements of individual single cells should provide necessary insight into the heterogeneity of metabolic activity of infected cells. Implementing 2p-FLIM to viral infection systems measuring NADH fluorescence at the single or subcellular level within a tissue can provide visual evidence, localization, and information in a real-time diagnostic or therapeutic metabolic workflow

For Me and My Gal / music by Geo W. Meyer; words by Edgar Leslie and E. Ray Goetz

Cover: photo of the Bowman Bros., two Caucasian males, one in blackface; Publisher: Waterson Berlin and Snyder Co. (New York)https://egrove.olemiss.edu/sharris_c/1038/thumbnail.jp

Crown Preservation of the Mandibular First Molar Tooth Impacts the Strength and Stiffness of Three Non-Invasive Jaw Fracture Repair Constructs in Dogs

Repairing mandibular body fractures present unique challenges not encountered when repairing long bones. Large tooth roots and the presence of the inferior alveolar neurovascular bundle limit safe placement for many types of orthopedic implants. Use of noninvasive fracture repair methods have increasingly become popular and have proven safe and effective at achieving bone healing. Noninvasive fixation constructs have not been tested in dogs using cantilevered bending. Furthermore noninvasive fracture repair constructs have not been tested at the location of a common fracture location- the mandibular first molar tooth (M1). The objectives of this study were to test the strength and stiffness of three noninvasive mandibular fracture repair constructs and to characterize the impact that tooth crown preservation has on fixation strength for fractures occurring at the M1 location. Specimens were assigned to three treatment groups: (1) composite only, (2) interdental wiring and composite and (3) transmucosal fixation screw and composite. For each pair of mandibles, one mandible received crown amputation at the alveolar margin to simulate the effect of crown loss on fixation strength and stiffness. Regardless of the status of crown presence, interdental wiring and composite demonstrated the greatest bending stiffness and load to failure. With the crown removed, interdental wiring and composite was significantly stronger compared to other treatments. All fixation constructs were stiffer when the tooth crown was preserved. In fractures at this location, retaining the tooth crown of M1 significantly increases stiffness of interdental wiring with composite and transmucosal screw with composite constructs. If the crown of M1 was removed, interdental wiring and composite was significantly stronger than the other two forms of fixation

Reply to “Comments on ‘The North American Regional Climate Change Assessment Program: Overview of Phase I Results\u27”

The authors of Mearns et al. (2012) are aware of the role of driving RCMs with reanalyses and have written extensively on the roles of different types of regional climate models (RCMs) simulations (e.g., Giorgi and Mearns 1999; Leung et al. 2003). Thus, we agree that the skill of dynamical downscaling in which global reanalysis is used to provide boundary conditions in general indicates an upper bound of skill compared to dynamical downscaling in which the boundary conditions come from global climate model simulations. This finding has long been established, as global climate model simulations cannot outperform global reanalysis in providing boundary conditions since the latter is constrained by observations through data assimilation (that is, unless the reanalyses themselves have been shown to have serious deficiences; e.g., Cerezo-Mota et al 2011). The classification of different types of dynamical downscaling introduced by Castro et al. (2005) further adds clarity to this point