A new search for interstellar C3

A new, very sensitive search for interstellar triatomic carbon has resulted in upper limits for a few diffuse clouds of order 1010 cm – 2, or about 10 – 11 with respect to hydrogen. These limits are consistent with recent cold diffuse cloud chemistry models, but may be in conflict with shocked cloud models such as those invoked to explain CH + abundances. Our results may also be argue against linear carbon-chain molecules as carriers of the diffuse interstellar bands

Predicting peculiar interstellar extinction from gaseous abundances

Molecular and atomic abundances are examined for 19 lines of sight through dense clouds, each with a peculiar selective extinction curve. The interstellar clouds in the present study appear to fall into two distinct categories: CN-rich, with relatively small amounts of neutral iron, or CN-poor, with large amounts of neutral iron. Lines of sight, having a CN/(Fe i) abundance ratio about two (~ 0.3 dex) or greater, are found to have a shallow (2.57 ± 0.55 mag) 2175 Å feature relative to the underlying extinction, while the strength of the bump is 3.60 ± 0.36 for the other dense clouds in the present study. The difference in the strength of the extinction bump between these two ensembles is 1.03 ± 0.23. Several atomic abundances are examined as potential indicators of peculiar extinction. Mn i abundances in particular are sought at 10 times greater sensitivity than previous studies because of a possible empirical connection between a small (Mn ii)/(Fe ii) abundance ratio and a weak 2175 Å bump reported in the literature. Unfortunately, the abundances of the neutral atoms do not appear to scale with the abundance of CN, reducing the effectiveness of Mn i as a diagnostic tool. Nevertheless, the Mn i upper limits in the present study support Mn being preferentially depleted. Fe i is underabundant relative to K i by 0.7 (dex) in the large (CN)/(Fe i) compared to the small (CN)/(Fe i) lines of sight. In addition, the data suggest that potassium is substantially depleted in both types of dense cloud

Predicting peculiar interstellar extinction from gaseous abundances

Molecular and atomic abundances are examined for 19 lines of sight through dense clouds, each with a peculiar selective extinction curve. The interstellar clouds in the present study appear to fall into two distinct categories: CN-rich, with relatively small amounts of neutral iron, or CN-poor, with large amounts of neutral iron. Lines of sight, having a CN/(Fe i) abundance ratio about two (~ 0.3 dex) or greater, are found to have a shallow (2.57 ± 0.55 mag) 2175 Å feature relative to the underlying extinction, while the strength of the bump is 3.60 ± 0.36 for the other dense clouds in the present study. The difference in the strength of the extinction bump between these two ensembles is 1.03 ± 0.23. Several atomic abundances are examined as potential indicators of peculiar extinction. Mn i abundances in particular are sought at 10 times greater sensitivity than previous studies because of a possible empirical connection between a small (Mn ii)/(Fe ii) abundance ratio and a weak 2175 Å bump reported in the literature. Unfortunately, the abundances of the neutral atoms do not appear to scale with the abundance of CN, reducing the effectiveness of Mn i as a diagnostic tool. Nevertheless, the Mn i upper limits in the present study support Mn being preferentially depleted. Fe i is underabundant relative to K i by 0.7 (dex) in the large (CN)/(Fe i) compared to the small (CN)/(Fe i) lines of sight. In addition, the data suggest that potassium is substantially depleted in both types of dense cloud

Ablation of an Ovarian Tumor Family Deubiquitinase Exposes the Underlying Regulation Governing the Plasticity of Cell Cycle Progression in \u3cem\u3eToxoplasma gondii\u3c/em\u3e

The Toxoplasma genome encodes the capacity for distinct architectures underlying cell cycle progression in a life cycle stage-dependent manner. Replication in intermediate hosts occurs by endodyogeny, whereas a hybrid of schizogony and endopolygeny occurs in the gut of the definitive feline host. Here, we characterize the consequence of the loss of a cell cycle-regulated ovarian tumor (OTU family) deubiquitinase, OTUD3A of Toxoplasma gondii (TgOTUD3A; TGGT1_258780), in T. gondii tachyzoites. Rather than the mutation being detrimental, mutant parasites exhibited a fitness advantage, outcompeting the wild type. This phenotype was due to roughly one-third of TgOTUD3A-knockout (TgOTUD3A-KO) tachyzoites exhibiting deviations from endodyogeny by employing replication strategies that produced 3, 4, or 5 viable progeny within a gravid mother instead of the usual 2. We established the mechanistic basis underlying these altered replication strategies to be a dysregulation of centrosome duplication, causing a transient loss of stoichiometry between the inner and outer cores that resulted in a failure to terminate S phase at the attainment of 2N ploidy and/or the decoupling of mitosis and cytokinesis. The resulting dysregulation manifested as deviations in the normal transitions from S phase to mitosis (S/M) (endopolygeny-like) or M phase to cytokinesis (M/C) (schizogony-like). Notably, these imbalances are corrected prior to cytokinesis, resulting in the generation of normal progeny. Our findings suggest that decisions regarding the utilization of specific cell cycle architectures are controlled by a ubiquitin-mediated mechanism that is dependent on the absolute threshold levels of an as-yet-unknown target(s). Analysis of the TgOTUD3A-KO mutant provides new insights into mechanisms underlying the plasticity of apicomplexan cell cycle architecture

Magnetic properties of pure and Gd doped EuO probed by NMR

An Eu NMR study in the ferromagnetic phase of pure and Gd doped EuO was performed. A complete description of the NMR lineshape of pure EuO allowed for the influence of doping EuO with Gd impurities to be highlighted. The presence of a temperature dependent static magnetic inhomogeneity in Gd doped EuO was demonstrated by studying the temperature dependence of the lineshapes. The results suggest that the inhomogeneity in 0.6% Gd doped EuO is linked to colossal magnetoresistance. The measurement of the spin-lattice relaxation times as a function of temperature led to the determination of the value of the exchange integral J as a function of Gd doping. It was found that J is temperature independent and spatially homogeneous for all the samples and that its value increases abruptly with increasing Gd doping.Comment: 14 pages, 10 figures, to be published in Physical Review

The Health Care Costs of Financial Exploitation in Maine

This study sought to determine the Medicare and Medicaid costs experienced by dual eligible older adults in Maine for whom Maine Adult Protective Services (APS) substantiated allegations of elder financial exploitation and to compare them to those of Maine’s general older population. The analysis is an important step forward in estimating the medical costs associated with elder abuse. Elder financial exploitation may result in significant public burden on Medicare and Medicaid, shouldered by taxpayers. Efforts to detect, investigate, prosecute, and mitigate this abuse will benefit not only the victims, but also the financial stewardship of these public programs

Effect of Composting on the Fate of Steroids in Beef Cattle Manure

In this study, the fate of steroid hormones in beef cattle manure composting is evaluated. The fate of 16 steroids and metabolites was evaluated in composted manure from beef cattle administered growth promotants and from beef cattle with no steroid hormone implants. The fate of estrogens (primary detected as estrone), androgens, progesterone, and the fusarium metabolite and implant a-zearalanol was monitored in manure compost piles. First-order decay rates were calculated for steroid half-lives in compost and ranged from 8 d for androsterone to 69 d for 4-androstenedione. Other steroid concentration data could not be fit to first-order decay models, which may indicate that microbial processes may result in steroid production or synthesis in composting systems. We demonstrate that composting is an effective strategy to remove steroid hormones from manure. Total steroid hormone removal in composted beef cattle manure ranged from 79 to 87%

Effect of rainfall timing and tillage on the transport of steroidhormones in runoff from manure amended row crop fields

Runoff generated from livestock manure amended row crop fields is one of the major pathways of hormone transport to the aquatic environment. The study determined the effects of manure handling, tillage methods, and rainfall timing on the occurrence and transport of steroid hormones in runoff from the row crop field. Stockpiled and composted manure from hormone treated and untreated animals were applied to test plots and subjected to two rainfall simulation events 30 days apart. During the two rainfall simulation events, detection of any steroid hormone or metabolites was identified in 8–86% of runoff samples from any tillage and manure treatment. The most commonly detected hormones were 17 _estradiol, estrone, estriol, testosterone, and _zearalenol at concentrations ranging up to 100–200 ng L−1. Considering the maximum detected concentrations in runoff, no more than 10% of the applied hormone can be transported through the dissolved phase of runoff. Results from the study indicate that hormones can persist in soils receiving livestock manure over an extended period of time and the dissolved phase of hormone in runoff is not the preferred pathway of transport from the manure applied fields irrespective of tillage treatments and timing of rainfall

Mosquito Abundance, Bed net Coverage and Other Factors Associated with Variations in Sporozoite Infectivity Rates in Four Villages of Rural Tanzania.

Entomological surveys are of great importance in decision-making processes regarding malaria control strategies because they help to identify associations between vector abundance both species-specific ecology and disease intervention factors associated with malaria transmission. Sporozoite infectivity rates, mosquito host blood meal source, bed net coverage and mosquito abundance were assessed in this study. A longitudinal survey was conducted in four villages in two regions of Tanzania. Malaria vectors were sampled using the CDC light trap and pyrethrum spray catch methods. In each village, ten paired houses were selected for mosquitoes sampling. Sampling was done in fortnight case and study was undertaken for six months in both Kilimanjaro (Northern Tanzania) and Dodoma (Central Tanzania) regions. A total of 6,883 mosquitoes were collected including: 5,628 (81.8%) Anopheles arabiensis, 1,100 (15.9%) Culex quinquefasciatus, 89 (1.4%) Anopheles funestus, and 66 (0.9%) Anopheles gambiae s.s. Of the total mosquitoes collected 3,861 were captured by CDC light trap and 3,022 by the pyrethrum spray catch method. The overall light trap: spray catch ratio was 1.3:1. Mosquito densities per room were 96.5 and 75.5 for light trap and pyrethrum spray catch respectively. Mosquito infectivity rates between villages that have high proportion of bed net owners and those without bed nets was significant (P < 0.001) and there was a significant difference in sporozoite rates between households with and without bed nets in these four villages (P < 0.001). Malaria remains a major problem in the study areas characterized as low transmission sites. Further studies are required to establish the annual entomological inoculation rates and to observe the annual parasitaemia dynamics in these communities. Outdoor mosquitoes collection should also be considered

Acute seizures attributable to falciparum malaria in an endemic area on the Kenyan coast

Falciparum malaria is an important cause of acute symptomatic seizures in children admitted to hospitals in sub-Saharan Africa, and these seizures are associated with neurological disabilities and epilepsy. However, it is difficult to determine the proportion of seizures attributable to malaria in endemic areas since a significant proportion of asymptomatic children have malaria parasitaemia. We studied children aged 0–13 years who had been admitted with a history of seizures to a rural Kenyan hospital between 2002 and 2008. We examined the changes in the incidence of seizures with the reduction of malaria. Logistic regression was used to model malaria-attributable fractions for seizures (the proportion of seizures caused by malaria) to determine if the observed decrease in acute symptomatic seizures was a measure of seizures that are attributable to malaria. The overall incidence of acute symptomatic seizures over the period was 651/100 000/year (95% confidence interval 632–670) and it was 400/100 000/year (95% confidence interval 385–415) for acute complex symptomatic seizures (convulsive status epilepticus, repetitive or focal) and 163/100 000/year (95% confidence interval 154–173) for febrile seizures. From 2002 to 2008, the incidence of all acute symptomatic seizures decreased by 809/100 000/year (69.2%) with 93.1% of this decrease in malaria-associated seizures. The decrease in the incidence of acute complex symptomatic seizures during the period was 111/100 000/year (57.2%) for convulsive status epilepticus, 440/100 000/year (73.7%) for repetitive seizures and 153/100 000/year (80.5%) for focal seizures. The adjusted malaria-attributable fractions for seizures with parasitaemia were 92.9% (95% confidence interval 90.4–95.1%) for all acute symptomatic seizures, 92.9% (95% confidence interval 89.4–95.5%) for convulsive status epilepticus, 93.6% (95% confidence interval 90.9–95.9%) for repetitive seizures and 91.8% (95% confidence interval 85.6–95.5%) for focal seizures. The adjusted malaria-attributable fractions for seizures in children above 6 months of age decreased with age. The observed decrease in all acute symptomatic seizures (809/100 000/year) was similar to the predicted decline (794/100 000/year) estimated by malaria-attributable fractions at the beginning of the study. In endemic areas, falciparum malaria is the most common cause of seizures and the risk for seizures in malaria decreases with age. The reduction in malaria has decreased the burden of seizures that are attributable to malaria and this could lead to reduced neurological disabilities and epilepsy in the area