Developing an Academic Portfolio for Initial Appointment and Promotion.

Objectives: 1.Describe the promotions process for faculty members 2.Identify ways to optimize your academic credentials to facilitate academic promotio

Insertional mutagenesis enables cleistothecial formation in a non-mating strain of Histoplasma capsulatum

<p>Abstract</p> <p>Background</p> <p><it>Histoplasma capsulatum </it>is a pathogenic ascomycete fungus that rapidly loses mating ability in culture. Loss of mating ability, as well as the organism's low rate of targeted gene replacement, limits techniques available for genetic studies in <it>H. capsulatum</it>. Understanding molecular mechanisms regulating mating in this organism may allow us to reverse or prevent loss of mating in <it>H. capsulatum </it>strains, introducing a variety of classical genetics techniques to the field. We generated a strain, UC1, by insertional mutagenesis of the laboratory strain G217B, and found that UC1 acquired the ability to form mating structures called cleistothecia. The aim of this study was to determine the mechanism by which UC1 gained the ability to form cleistothecia. We also present initial studies demonstrating that UC1 can be used as a tool to determine molecular correlates of mating in <it>H. capsulatum</it>.</p> <p>Results</p> <p>The strain UC1 was found to have increased RNA levels of the mating locus transcription factor (<it>MAT1-1-1</it>), and the putative alpha pheromone (<it>PPG1</it>) compared to G217B. <it>Agrobacterium</it>-mediated transformation and integration of T-DNA from the vector pCB301-GFP-HYG were found to be partially responsible for the increased RNA levels of these genes; however, the site of integration appeared to play the largest role in the strain's ability to form cleistothecia. Silencing <it>HMK1</it>, a putative <it>FUS3/KSS1 </it>homolog, had no effect on cleistothecial production by UC1. Protein kinase C <it>(PKC1) </it>RNA and protein levels were increased in UC1 compared to G217B, and pheromone production was found to be linked with Pkc1 activity in <it>H. capsulatum</it>.</p> <p>Conclusions</p> <p>The site of the T-DNA integration event appears to play the largest role in UC1's ability to form cleistothecia. We show that the UC1 strain can be used as a tool to study cleistothecia production in <it>H. capsulatum </it>by manipulating the strain, or by identifying differences between UC1 and G217B. Using these approaches, we were able to link Pkc1 activity with pheromone production in <it>H. capsulatum</it>; however, further studies are required to determine molecular mechanisms behind this. These studies may reveal regulatory mechanisms that can be manipulated to restore mating ability in <it>H. capsulatum </it>laboratory strains.</p

Sonographic Findings in Acute Uterine Inversion

We present a case of acute uterine inversion in the third stage of labor in which critical management decisions were facilitated by ultrasound imaging in the operating room. Identification of the ovary and adnexa pulled into the inclination of the inversion allowed successful diagnosis and guidance of uterine replacement

A topological approach to Best Approximation Theory

[EN] The main goal of this paper is to put some light in several arguments that have been used through the time in many contexts of Best Approximation Theory to produce proximinality results. In all these works, the main idea was to prove that the sets we are considering have certain properties which are very near to the compactness in the usual sense. In the paper we introduce a concept (the wrapping) that allow us to unify all these results in a whole theory, where certain ideas from Topology are essential. Moreover, we do not only cover many of the known classical results but also prove some new results. Hence we prove that exists a strong interaction between General Topology and Best Approximation Theory.Research supported by Junta de Andaluc´ıa, Grupos FQM0178 and FQM0268.Moreno, SG.; Almira, JM.; García-Caballero, EM.; Quesada, J. (2004). A topological approach to Best Approximation Theory. Applied General Topology. 5(1):49-70. https://doi.org/10.4995/agt.2004.1994SWORD49705