Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta)

Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection

Pulsed electrochemical synthesis of formate using Pb electrodes

Lead cathodes show decreasing Faradaic Efficiency (FE) in electrochemical conversion of CO2 to formate, favoring hydrogen, within minutes of operation in KHCO3 electrolyte at −1 to −1.3 V vs RHE. Periodic anodic polarization (pulsed electrochemistry) is demonstrated to result in a high time-averaged FE towards formate. Specifically, an anodic polarization time of 0.1–1 s is sufficient to obtain a Pb-surface providing an averaged formate FE of 30–50%, when the cathodic polarization time is limited to a few seconds. A Pourbaix diagram and Raman spectra are provided, which show that PbCO3 is formed on the surface of the cathode in KHCO3 electrolyte at anodic potential (0.05 V vs RHE), which compound is likely inducing the high FE towards formate. Our method thus provides a means to operate Pb electrodes in electrochemical CO2 reduction with high stability, at a low energy penalty

Immobilization of the C-terminal extension of bovine alpha A-crystallin reduces chaperone-like activity

Contains fulltext : 28223.pdf (Publisher’s version ) (Open Access

Track 5.b Introduction: Strengthening the Design Capabilities of Professional Organisations in a Complex World

In Hong Kong of the year 2017, a new academic community convened to attend to pressing issues regarding design as source of innovation. The inaugural Academy for Design Innovation Management Conference (nee Design Management Academy) attended to a sense of urgency regarding the adoption of design capabilities within organisations as source of innovation. The title of the conference, Research perspectives on creative intersections was therefore pertinent, with papers exploring how design and designers were intersecting with new business challenges. Two years later in London (2019), rhetoric has notably shifted from matters of adoption to strengthening design capabilities within organisations, thereby enabling those organisations to unlock the possibilities and subsequent benefits of design. These possibilities include but are not limited to strategic and cultural renewal, design of new processes and meaningful engagement with hard-to-reach stakeholders.To address the complex nature of today’s societal and economic problems, professional organisations now recognize that traditional tools and approaches may not provide the required solutions. To address complex challenges, many managers and business leaders have consciously turned to design approaches over the past decade, including both public and private sectors. To increase design capabilities, these organisations have established innovation labs with designers, have recruited designers in strategic positions, and/or have started building the design competence of existing staff through educational programs, often provided by design consultancies. Yet to date, describing the resultant impact of teaching. Individual design competencies on organisational design capabilities has proven elusive.Methodology and Organisation of DesignMarketing and Consumer Researc

The mammalian small heat-shock protein Hsp20 forms dimers and is a poor chaperone.

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Validity and repronducibility of electrical impedance tomography for measurement of calf blood flow in healthy subjects

The Sheffield electrical impedance tomography; (EIT) system produces images of changes in the distribution of resistivity within tissue. The paper reports on the application of electrical impedance tomography in monitoring volume changes in the limb during venous occlusion. The aim of the study is to assess the feasibility, reproducibility and validity of calf blood flow measurements by EIT. In 14 healthy volunteers calf blood flow is compared, as determined in a calf segment by strain-gauge plethysmography (SGP), with the impedance changes measured by EIT during rest and post-ischaemic hyperaemia. The measurements are repeated to assess reproducibility. The reproducibility for the EIT, assessed from the repeated measurements and expressed as a reproducibility coefficient, is 0.88 during rest and 0.89 during hyperaemia. The reproducibility coefficient for SGP data is 0.83 at rest and 0.67 during hyperaemia. Flow measurements, assessed by means of two methods, correlate well at rest (r = 0.89), but only moderately during hyperaemia (r = 0.51). The correlation coefficient for the pooled flow measurements is 0.98. It is concluded that EIT is a valid and reliable method for assessing blood flow in the limb. Possible applications of EIT in localising fluid changes are discusse