Degrading of nitrobodies using microorganisms

In this study biodegradation of p-nitrophenol (PNP) by soil microorganisms is evaluated. In biological degradation, performance of pure culture and  mixed culture was investigated. It has been observed that the cultivable flora of soil sample was 4x10 7cfu/gm which could utilize PNP 100 ppm  as energy source. The organisms were isolated ,identified and screened on the basis of maximum PNP degradation with least time at room temperature. Five bacterial  isolates  were selected on the basis of their ability to  tolerate  and degrade PNP 1000ppm. Isolate PD07 could be able to grow and reach absorbance 1 up to within 17 hrs at room temperature. Growth profile of the this isolate showed maximum growth in presence of Nutrient broth and PNP. As minimal media (without nitrogen and carbon source ) was used for whole study it has been concluded that this organism utilizes PNP as carbon and nitrogen source.

Evaluation of antimicrobial activity of probiotic microflora from neonatal origin.

The antimicrobial effects of the bacteriocin obtained from nine Lactic Acid Bacteria isolated from the fecal sample of breastfed neonates were tested against three pathogenic indicator strains, Shigella flexineri NCIM5265, Bacillus cereus NCIM2079 and Proteus vulgaris NCIM2027. 20 hrs old cultures supernatant was used to perform the antimicrobial activity using well diffusion method. All the nine isolates showed good inhibition of Shigella flexineri and Bacillus cereus. While seven isolates of nine showed moderate inhibition of Proteus vulgaris .Two isolate showed no inhibition to Proteus vulgaris

Factors affecting gut microbiota in early infancy

The colonization of the infant gut starts prior to birth and continues throughout the life. Specific factors may influence the microbial composition in infants. A Particular focus  of interest of this review article was the impact of specific factors on development of gut microbiota  in early infancy such as mother’s gut microbiota, mode of delivery , mode of feeding , hygieneic measures, antibiotic administration in early infancy which may influence this process