Диагностическое и прогностическое значения ядрышковых белков в регуляции пролиферации опухолевых клеток у больных с карциномой легких и адренокортикальным раком

We studied correlation between the degree of malignancy, prognosis, survival of oncological patients, and the number of proliferating cells (Ki-67 index), the rate of cell proliferation, the continuance of the mitotic cycle (and the expression of Ag-OYAOR-proteins-В23/nucleofozimine S23/nukleoline) in 16 patients with adrenocortical carcinoma and in 20 cases of “small” lung cancer (up to 3 cm in diameter), whose tumors were removed and long-term results were evaluated. The comparative study of proliferative activity of cells in the two groups of patients with different survival period after tumor removal showed the heterogeneity of Ki-67 index, which determined the number of proliferating cells. In the first group of patients (with a good prognosis) Ki-67 index was high, and expression of Ag-OYAOR proteins was low. At the same time, in the second group of patients, whose prognosis was poor, Ki-67 index in the tumor was low, but the expression of Ag-OYAOR-protein was high. So, we can make a conclusion, that it is impossible to discuss the degree of malignancy of the tumor and the prognosis, based only on Ki-67 index. Ag-OYAOR-protein and Ki-67 index reflect different aspects of cell proliferation: the rate of cell proliferation (Ag-OYAOR expression) and the number of proliferating cells (Ki-67 index).Изучены значения при определении степени злокачественности, прогноза, выживаемости онкологических больных, одновременного исследования количества пролиферирующих клеток (индекс Ki-67) и определения скорости клеточной пролиферации — длительности митотического цикла (экспрессия Ag-ОЯОР-белков — В23/нуклеофозмина и С23/нуклеолина) у 16 больных с адренокортикальной карциномой и 20 наблюдений так называемых «малых» раков легкого (до 3 см в диаметре), удаленных у пациентов с известными отдаленными результатами. Проведенное сравнительное исследование пролиферативной активности клеток в двух группах больных с различным сроком выживаемости после удаления опухоли показало неоднородность индекса Ki-67, определяющего количество пролиферирующих клеток. В первой группе (с благоприятным прогнозом) у части больных индекс Ki-67 в опухолях был высоким, а экспрессия Ag-ОЯОР-белков низкая. В то же время у ряда больных второй группы (с неблагоприятным прогнозом) индекс Ki-67 в опухоли был низким, но экспрессия Ag-ОЯОР-белков высокой. Следовательно исследование только индекса Ki-67 не всегда свидетельствует о степени злокачественности опухоли и прогнозе заболевания. Ag-ОЯОР-белки и индекс Ki-67 отражают разные аспекты пролиферативной активности клеток: скорость клеточной пролиферации (экспрессия Ag-ОЯОР) и количество пролиферирующих клеток (индекс Ki-67)

Revised data on γ-families observed in X-ray emulsion chambers of the Experiment PAMIR

Recently essential efforts were made to improve measurement routine with X-ray films exposed in the X-ray emulsion chambers at the Pamirs. Analysis of X-ray emulsion response upon recorded events show that γ-family energy and intensity in early publications were over estimated. The main physical results of the new analysis are presented

Estimation of Hydrodynamical Model Parameters from the Invariant Spectrum and the Bose-Einstein Correlations of pi-mesons Produced in (pi+/K+)p Interactions at 250 GeV/c

The invariant spectra of pi- mesons produced in (pi+/K+)p interactions at 250 GeV/c are analysed in the framework of the hydrodynamical model of three-dimensionally expanding cylindrically symmetric finite systems. A satisfactory description of experimental data is achieved. The data favour the pattern according to which the hadron matter undergoes predominantly longitudinal expansion and non-relativistic transverse expansion with mean transverse velocity = 0.20(7), and is characterized by a large temperature inhomogeneity in the transverse direction: the extracted freeze-out temperature at the center of the tube and at the transverse rms radius are 140(3) MeV and 82(7) MeV, respectively. The width of the (longitudinal) space-time rapidity distribution of the pion source is found to be Delta eta = 1.36(2). Combining this estimate with results of the Bose-Einstein correlation analysis in the same experiment, one extracts a mean freeze-out time of the source of = 1.4(1) fm/c and its transverse geometrical rms radius, R_G (rms)=1.2(2) fm.Comment: latex, 14 pages, 5 figure

Atomic structure at 2.5 Å resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice

AbstractUridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 Å resolution. The coordinates of the trigonal crystal form were used as a starting model and the refinement by the program XPLOR led to the R-factor of 18.6%. The amino acid fold of the protein was found to be the same as that in the trigonal crystals. The positions of flexible regions were refined. The conclusion about the involvement in the active site is in good agreement with the results of the biochemical experiments

Boost Invariance and Multiplicity Dependence of the Charge Balance Functionin π+p\pi^{+}p and K+pK^{+}p Collisions at s=22\sqrt s= 22 GeV/c

Boost invariance and multiplicity dependence of the charge balance function are studied in \pi^{+}\rp and \rK^{+}\rp collisions at 250 GeV/$c$ incident beam momentum. Charge balance, as well as charge fluctuations, are found to be boost invariant over the whole rapidity region, but both depend on the size of the rapidity window. It is also found that the balance function becomes narrower with increasing multiplicity, consistent with the narrowing of the balance function when centrality and/or system size increase, as observed in current relativistic heavy ion experiments.Comment: 4 pages, 5 figures, Revte

Manifestations of Higher Mental Functions in Actors in Virtual Reality

Background. The study of the professional activities of actors from the standpoint of modern psychological theories is an urgent task for psychology of art. In particular, the question of whether there is a significant difference in the functioning of cognitive processes in actors as a result of training in acting techniques is considered. The answer can be revealed not only by classical measurements of the development of higher mental functions using standardized methods, but also, to a large extent, by the assessment of cognitive abilities in special conditions of virtual reality. Objectives. The study is aimed at measuring success parameters for actors performing tasks to assess the level of cognitive functioning in virtual reality in comparison with a group of students who are not actors. Methods. During the experiment, the control (students who are not actors) and experimental (novice actors) groups were asked to complete tasks to assess the level of cognitive functioning in two-dimensional and virtual reality formats. In order to do this, the experiment participants first performed two-dimensional tests to assess visual attention and working memory, and then did tasks to assess attention and memory in the HTC Vive Pro virtual reality helmet in a special virtual environment “Upgrade VR”. Sample. Two groups of subjects took part in the experiment. The experimental group consisted of 10 students of the O. Tabakov School (average age 17 years). The control group included 20 subjects (18 women, 2 men, average age 18 years) — students of the Faculty of Psychology at Lomonosov Moscow State University. Results. Comparing the performance of actors before and after immersion in virtual reality on two-dimensional computerized task assessing working memory, no significant differences were found (t = –1.35, df = 9, p = 0.209). Also, no significant differences were found when comparing the results of actors performing tasks to assess attention parameters (t = –0.070, df = 9, p = 0.946). When comparing the results of actors and students in performing tasks in virtual reality, significant differences were revealed in the success of completing a spatial orientation task (t = –4.44, df = 28, p < 0.001) and a task assessing working memory (t = –2.34, df = 28, p = 0.027). In addition, significant differences were identified in the average success rates in completing two-dimensional tasks between actors and students before immersion in VR according to the results of the N-back technique (t = 2.4, df = 28, p < 0.023) and the SART technique (t = –3.95, df = 28, p < 0.001). Conclusions. The study revealed differences in parameters of cognitive functioning between actors and students, especially noticeable when performing tasks in virtual reality related to spatial orientation and working memory. The use of virtual reality allows you to simulate a stage situation and create training conditions for the development of cognitive skills, reactions to unexpected events and emotional expression of actors. The results of the study showed that the use of digital technologies in art opens up new opportunities for the creative process and for the construction of unique artistic images

Entropy Analysis in \pi^{+}\rp and \rK^{+}\rp Collisions at s=22\sqrt{s}=22 GeV

The entropy properties are analyzed by Ma's coincidence method in \pi^{+}\rp and \rK^{+}\rp collisions of the NA22 experiment at 250 GeV/$c$ incident momentum. By using the R\'{e}nyi entropies, we test the scaling law and additivity properties in rapidity space. The behavior of the R\'{e}nyi entropies as a function of the average number of particles is investigated. The results are compared with those from the {\sc Pythia} Monte Carlo event generator.Comment: LaTeX, 11 pages, 5 figure to be appeared in Acta Phys. Pol.

Screened Coulomb interactions in metallic alloys: II Screening beyond the single-site and atomic sphere approximations

A quantitative description of the configurational part of the total energy of metallic alloys with substantial atomic size difference cannot be achieved in the atomic sphere approximation: It needs to be corrected at least for the multipole moment interactions in the Madelung part of the one-electron potential and energy. In the case of a random alloy such interactions can be accounted for only by lifting the atomic sphere and single-site approximations, in order to include the polarization due to local environment effects. Nevertheless a simple parameterization of the screened Coulomb interactions for the ordinary single-site methods, including the generalized perturbation method, is still possible. We obtained such a parameterization for bulk and surface NiPt alloys, which allows one to obtain quantitatively accurate effective interactions in this system.Comment: 24 pages, 2 figure