76 research outputs found
[Libraries of large-insert genomic clones as a tool for molecular cytogenetic analysis of avian genome] ΠΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ Π³Π΅Π½ΠΎΠΌΠ½ΡΡ ΠΊΠ»ΠΎΠ½ΠΎΠ² ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π³Π΅Π½ΠΎΠΌΠ° ΠΏΡΠΈΡ
Integration of molecular and cytegenetic levels of investigation results in complex understanding of structural and functional genome organization. Gridded libraries of large-insert genomic clones represent a powerful tool of the genome analysis. Their utilization provides coordination of data on molecular organization of nucleic acids with cytogenetic data on the chromosome structure. These libraries played an important role in sequencing of genomes of human, mouse, and other organisms as an instrument linking molecular biological and cytogenetic data via construction of contigs and their localization on the chromosomes. They also enabled analysis of orthology between the mammalian genomes. The existing avian libraries fit molecular cytogenetic analysis of the class Aves genome, and can be successfully used for the isolation and characterization of large genomic fragments. This provides utilization of these libraries not only for the chromosome mapping, but also for positional cloning and search for candidate genes for quantitative traits.
Π‘Π°Π·Π°Π½ΠΎΠ² Π.Π., Π ΠΎΠΌΠ°Π½ΠΎΠ² Π.Π., Π‘ΠΌΠΈΡΠ½ΠΎΠ² Π.Π€.
ΠΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΠΈ ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Π΅ΠΉ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ Π²Π΅Π΄Π΅Ρ ΠΊ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΌΡ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π³Π΅Π½ΠΎΠΌΠ°. ΠΡΠΈΠ΄ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ
Π³Π΅Π½ΠΎΠΌΠ½ΡΡ
ΠΊΠ»ΠΎΠ½ΠΎΠ² ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΠΌΠΎΡΠ½ΡΠΉ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ Π°Π½Π°Π»ΠΈΠ·Π° Π³Π΅Π½ΠΎΠΌΠ°. ΠΡ
ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π΄Π°Π΅Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΏΡΠΈΠ²Π΅ΡΡΠΈ Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠ΅ Π΄ΡΡΠ³ Ρ Π΄ΡΡΠ³ΠΎΠΌ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ ΠΈ ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎ ΡΡΡΡΠΊΡΡΡΠ΅ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ. Π’Π°ΠΊΠΈΠ΅ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΡΡΠ³ΡΠ°Π»ΠΈ Π±ΠΎΠ»ΡΡΡΡ ΡΠΎΠ»Ρ Π² ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ°Ρ
ΠΏΠΎΠ»Π½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π³Π΅Π½ΠΎΠΌΠΎΠ² ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, Π΄ΠΎΠΌΠΎΠ²ΠΎΠΉ ΠΌΡΡΠΈ ΠΈ Π΄ΡΡΠ³ΠΈΡ
ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΊΠ°ΠΊ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ ΡΠ²ΡΠ·ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΏΡΡΠ΅ΠΌ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠΈΠ³ΠΎΠ² ΠΈ ΠΈΡ
Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π½Π° Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΠ°Ρ
, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ Π³Π΅Π½ΠΎΠΌΠ°ΠΌΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
. Π‘ΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ½ΡΠ΅ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΠΏΡΠΈΡ ΡΠ²Π»ΡΡΡΡΡ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΡΠΌΠΈ Π·Π°Π΄Π°ΡΠ°ΠΌ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π³Π΅Π½ΠΎΠΌΠ° ΠΊΠ»Π°ΡΡΠ° Aves ΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ Ρ ΡΡΠΏΠ΅Ρ
ΠΎΠΌ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ
ΡΡΠ°ΡΡΠΊΠΎΠ² Π³Π΅Π½ΠΎΠΌΠ°. ΠΡΠΎ ΠΎΠ±ΡΡΠΎΡΡΠ΅Π»ΡΡΡΠ²ΠΎ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΈΠΌ Π½Π°ΠΉΡΠΈ ΡΠ²ΠΎΠ΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΊΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ, Π½ΠΎ ΠΈ Π΄Π»Ρ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΏΠΎΠΈΡΠΊΠ° Π³Π΅Π½ΠΎΠ²-ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠΎΠ² ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ²
[Genomic assessment of breeding bulls] ΠΠ΅Π½ΠΎΠΌΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° ΠΏΠ»Π΅ΠΌΠ΅Π½Π½ΡΡ Π±ΡΠΊΠΎΠ²
The review considers aspects of genomic assessment of breeding bulls based on the use of molecular genetic markers and, in particular, SNP markers for determining the breeding value of animals.
Π ΠΎΠ±Π·ΠΎΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π°ΡΠΏΠ΅ΠΊΡΡ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΠ»Π΅ΠΌΠ΅Π½Π½ΡΡ
Π±ΡΠΊΠΎΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΈ, Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ, SNP-ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΏΠ»Π΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΡΠ΅Π½Π½ΠΎΡΡΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Libraries of large-insert genomic clones as a tool for molecular cytogenetic analysis of avian genome
Integration of molecular and cytegenetic levels of investigation results in complex understanding of structural and functional genome organization. Gridded libraries of large-insert genomic clones represent a powerful tool of the genome analysis. Their utilization provides coordination of data on molecular organization of nucleic acids with cytogenetic data on the chromosome structure. These libraries played an important role in sequencing of genomes of human, mouse, and other organisms as an instrument linking molecular biological and cytogenetic data via construction of contigs and their localization on the chromosomes. They also enabled analysis of orthology between the mammalian genomes. The existing avian libraries fit molecular cytogenetic analysis of the class Aves genome, and can be successfully used for the isolation and characterization of large genomic fragments. This provides utilization of these libraries not only for the chromosome mapping, but also for positional cloning and search for candidate genes for quantitative traits
First century of chicken gene study and mapping β a look back and forward
Chicken gene inheritance analysis, started one century ago, had led to the development of the classical genetic map. Efforts and legacy of the previous geneticists' generations are not forgotten and constitute the fundamentals of contemporary genome research progress. Advances in molecular biology, cytogenetics and DNA technologies provided more powerful and sophisticated tools to tackle chicken gene mapping and genome research problems. In the 1990s configurations of chicken molecular and cytogenetic maps had begun standing out. New horizons in chicken genomics are opening with application of BAC libraries, BAC-contig physical maps, ESTs and whole genome sequencing. The chicken has been a notable experimental model for several fundamental and applied biologic disciplines in the last century, and will remain such in the 21st century. The upcoming complete genome sequencing combined with discovering gene functions will facilitate the improvement of traits of economic importance and value in poultry
Poultry
From the time of initial domestication of wild birds, poultry have served humans as a source of food and a subject of cultural use, similar to other livestock species. The availability of genomic resources ranges from a fully annotated whole genome sequence for the chicken (Gallus gallus) to highly developed linkage maps in Japanese quail (Coturnix japonica), to large insert libraries and at least some species-specific markers in the turkey (Meleagris gallopavo), domestic duck (Anas platyrhynchos), zebra finch (Taeniopygia guttata), brown kiwi (Apteryx australis), and California condor (Gymnogyps californianus). Genomic resources are very limited in other species of birds. The annotated chicken genome sequence will pave the way for improving traits of economic importance in the chicken, and will serve as a reference sequence in comparative mapping of other domesticated and wild bird species
[Positional cloning of chromosomal regions controlling QTLs in chickens] ΠΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ ΡΠΎΠΌΠΎΡΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ Ρ ΠΊΡΡ
Π‘Π°Π·Π°Π½ΠΎΠ² Π.Π.; Π¦Π°ΡΠ΅Π²Π° Π.Π.; Π‘ΠΌΠΈΡΠ½ΠΎΠ² Π.Π€.; ΠΠ°ΡΠ΄Π΅ΡΠΊΠ° Π.; ΠΠΎΡΡΠ°ΠΊ Π.; Π―ΡΠ°ΠΊ Π.; Π ΠΎΠΌΠ°Π½ΠΎΠ² M.Π.
ΠΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²ΠΎ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π΄ΠΎΠΌΠ°ΡΠ½ΠΈΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΈΠΌΠ΅ΡΡ ΡΠ»ΠΎΠΆΠ½ΡΠΉ ΠΏΠΎΠ»ΠΈΠ³Π΅Π½Π½ΡΠΉ ΡΠΈΠΏ Π½Π°ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΡΡ ΠΌΠ½ΠΎΠ³ΠΈΠΌΠΈ Π³Π΅Π½Π°ΠΌΠΈ, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠΌΠΈ Π² Π»ΠΎΠΊΡΡΠ°Ρ
QTL (quantitative trait loci). ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΉ Π°ΡΡ
ΠΈΡΠ΅ΠΊΡΡΡΡ QTL ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ Ρ ΡΠΎΡΠΊΠΈ Π·ΡΠ΅Π½ΠΈΡ ΠΎΠ±ΡΠ΅ΠΉ Π³Π΅Π½Π΅ΡΠΈΠΊΠΈ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄Π°Π½Π½ΡΠ΅ ΠΎ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ
ΠΈΠ· ΡΠ°ΠΉΠΎΠ½ΠΎΠ² QTL ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π² ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΠΎΠ²ΠΎΠ΄ΡΡΠ²Π΅ Π΄Π»Ρ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² (marker assisted selection, MAS).
Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π½Π°ΠΌΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ΄ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌΡ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄Π²ΡΡ
ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ QTL ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΊΠΎΡΠ»ΡΠΏΡ Π½Π° 53 Π½Π΅Π΄Π΅Π»ΠΈ ΠΆΠΈΠ·Π½ΠΈ (ST53) ΠΈ ΠΌΠ°ΡΡΡ Π±Π΅Π»ΠΊΠ° Π² ΡΠΉΡΠ΅ Π½Π° 33 Π½Π΅Π΄Π΅Π»Π΅ (AW33). Π£ΠΊΠ°Π·Π°Π½Π½ΡΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΡΡ Ρ Π΄Π²ΡΡ
Π»ΠΈΠ½ΠΈΠΉ ΠΊΡΡ (ΠΏΠΎΠ»ΡΡΠΊΠ°Ρ Π·Π΅Π»Π΅Π½ΠΎΠ½ΠΎΠ³Π°Ρ ΠΈ ΡΠΎΠ΄-Π°ΠΉΠ»Π΅Π½Π΄) Π½Π° 3,3% ΠΈ 7,5%, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ° AW33 Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ MCW170 (Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΠ΅ 1ΡΠ) ΠΈ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΠΏΠΎΠ»Π½ΠΎΠ΅ ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ QTL ST53 Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ MCW114. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±Π°Π· Π΄Π°Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠΈ ΠΠ½ΡΠ΅ΡΠ½Π΅Ρ ΠΏΠΎΠΊΠ°Π·Π°Π½Π° Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ° AW33 Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»Π°, ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170 ΠΈ LEI0081, ΠΈ QTL ST53 Π²Π½ΡΡΡΠΈ ΡΠ°ΠΉΠΎΠ½Π° Ρ Π³ΡΠ°Π½ΠΈΡΠ°ΠΌΠΈ MCW0114 ΠΈ ADL0241. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ Π³ΡΠΈΠ΄ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ BAC-Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΠΊΡΡΠΈΡΡ 031-JF256-BI (http://hbz.tamu.edu) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΠΠ-Π·ΠΎΠ½Π΄ΠΎΠ² ΠΌΠ΅ΡΠ΅Π½ΡΡ
-32P-dCTP ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠΎΠ² MCW0170, LEI0081, MCW0114 ΠΈ ADL0241. ΠΡΠ°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΡΠΊΠ°Π½Π΅ΡΠ° FX-scan ΠΈ ΠΏΠ°ΠΊΠ΅ΡΠ° ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΡ
ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌ Quantity One. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΡ Π΄Π²Π°Π΄ΡΠ°ΡΠΈ ΠΊΠ»ΠΎΠ½ΠΎΠ², ΠΈΠΌΠ΅ΡΡΠΈΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΠΠ Π²ΡΡΠ°Π²ΠΊΠΈ Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170, LEI0081, MCW0114 ΠΈ ADL0241.
ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ Β«Π·Π°ΡΠΊΠΎΡΠΈΡΡΒ» ΡΠ°ΠΉΠΎΠ½Ρ QTL Π½Π° Π΄Π΅ΡΠ°Π»ΡΠ½ΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΡ
ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ
Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ°ΡΡΠ°Ρ
Β«ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ-ΠΌΡΡΡΒ» Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ, Π²ΡΡΠ²ΠΈΡΡ Π³Π΅Π½Ρ, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° QTL ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΊΠΎΡΠ»ΡΠΏΡ ΠΈ ΠΌΠ°ΡΡΡ Π±Π΅Π»ΠΊΠ° Π² ΡΠΉΡΠ΅. ( ΠΠ°Π½Π΄ΠΈΠ΄Π°ΡΡΠΊΠΈΠ΅ ΠΏΡΠΎΠ΅ΠΊΡΡ.
[Positional cloning of quantitative trait loci in the domestic fowl] ΠΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π»ΠΎΠΊΡΡΠΎΠ² ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Ρ Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ
ΠΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²ΠΎ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π΄ΠΎΠΌΠ°ΡΠ½ΠΈΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΈΠΌΠ΅ΡΡ ΡΠ»ΠΎΠΆΠ½ΡΠΉ ΠΏΠΎΠ»ΠΈΠ³Π΅Π½Π½ΡΠΉ ΡΠΈΠΏ Π½Π°ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΡΡ ΠΌΠ½ΠΎΠ³ΠΈΠΌΠΈ Π³Π΅Π½Π°ΠΌΠΈ, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠΌΠΈ Π² Π»ΠΎΠΊΡΡΠ°Ρ
QTL (quantitative trait loci). ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ΄ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌΡ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄Π²ΡΡ
ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ QTL ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΊΠΎΡΠ»ΡΠΏΡ Π½Π° 53 Π½Π΅Π΄Π΅Π»ΠΈ ΠΆΠΈΠ·Π½ΠΈ (ST53) ΠΈ ΠΌΠ°ΡΡΡ Π±Π΅Π»ΠΊΠ° Π² ΡΠΉΡΠ΅ Π½Π° 33 Π½Π΅Π΄Π΅Π»Π΅ (AW33). Π£ΠΊΠ°Π·Π°Π½Π½ΡΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΡΡ Ρ Π΄Π²ΡΡ
Π»ΠΈΠ½ΠΈΠΉ ΠΊΡΡ (ΠΏΠΎΠ»ΡΡΠΊΠ°Ρ Π·Π΅Π»Π΅Π½ΠΎΠ½ΠΎΠ³Π°Ρ ΠΈ ΡΠΎΠ΄-Π°ΠΉΠ»Π΅Π½Π΄) Π½Π° 3,3% ΠΈ 7,5%, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠΎΡΠ΅Π³ΡΠ΅Π³Π°ΡΠΈΠΈ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΡ
ΠΠΠ-ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΌΠ°ΡΠΊΠ΅ΡΡ ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»ΠΎΠ² Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΡ (ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ), ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΡ
QTL AW33 ΠΈ ST53. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±Π°Π· Π΄Π°Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠΈ ΠΠ½ΡΠ΅ΡΠ½Π΅Ρ ΠΏΠΎΠΊΠ°Π·Π°Π½Π° Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ° AW33 Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»Π°, ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170 ΠΈ LEI0081, ΠΈ QTL ST53 Π²Π½ΡΡΡΠΈ ΡΠ°ΠΉΠΎΠ½Π° Ρ Π³ΡΠ°Π½ΠΈΡΠ°ΠΌΠΈ MCW0114 ΠΈ ADL0241. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΡ Π΄Π²Π°Π΄ΡΠ°ΡΠΈ ΠΊΠ»ΠΎΠ½ΠΎΠ², ΠΈΠΌΠ΅ΡΡΠΈΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΠΠ Π²ΡΡΠ°Π²ΠΊΠΈ Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170, LEI0081, MCW0114 ΠΈ ADL0241. ΠΠ΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π°ΡΡΠ΅Π½ΡΠΈΡΠ½ΠΎΡΡΠΈ ΠΊΠ»ΠΎΠ½ΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π΄Π»Ρ ΡΡΠ΅Ρ
ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΡ
Π»ΠΎΠΊΡΡΠΎΠ² (MCW0170, MCW0114 ΠΈ ADL0241) ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠ΅ Π΄Π²ΡΡ
BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ² Π»ΠΎΠΊΡΡΡ MCW0170 (QTL AW33), Π΄Π΅Π²ΡΡΠΈ β Π»ΠΎΠΊΡΡΡ MCW0114 (QTL ST53) ΠΈ ΡΠ΅ΡΡΡΠ΅Ρ
BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ² Π»ΠΎΠΊΡΡΡ ADL0241 (QTL ST53). ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠΉ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ ΠΠΠ-ΠΠΠ in situ (FISH) ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π²Π½ΡΡΡΠΈΡ
ΡΠΎΠΌΠΎΡΠΎΠΌΠ½Π°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ 15-ΡΠΈ BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠ΅ Π»ΠΎΠΊΡΡΡ. ΠΡΡ
ΠΎΠ΄Ρ ΠΈΠ· ΡΡΠ΅Π΄Π½Π΅Π³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ° Π²ΡΡΠ°Π²ΠΊΠΈ β 150 Ρ.ΠΏ.Π½. β ΠΌΠΎΠΆΠ½ΠΎ Π³ΠΎΠ²ΠΎΡΠΈΡΡ ΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π΄Π²ΡΡ
ΡΡΠ°ΡΡΠΊΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΉ Π΄Π»ΠΈΠ½ΠΎΠΉ 300 Ρ.ΠΏ.Π½. Π΄Π»Ρ QTL AW33 ΠΈ 1950 Ρ.ΠΏ.Π½. Π΄Π»Ρ QTL ST53
Localization of seven HSA3q13-q23 NotI linking clones on the chicken microchromosomes 14 and 15 by double-color FISH
Double-color fluorescence in situ hybridization was performed on chicken chromosomes using seven unique clones from human chromosome 3-specific NotI linking libraries. Six of them (NL1-097, NL2-092, NL2-230, NLM-007, NLM-118, and NLM-196) hybridized to the same chicken microchromosome, while NL1-290 hybridized to another one. Two chicken microchromosome GGA15-specific BAC clones, JE024F14 containing the IGVPS gene and JE020G17 containing the ALDH1A1 gene, were cytogenetically mapped to the same microchromosome that contained the six NotI linking clones, allowing identification of this chromosome as GGA15. Two GGA14-specific clones, JE027C23 and JE014E08, containing the HBA gene cluster, were co-localized on the same microchromosome with NL1-290, marking this chromosome as GGA14. The results indicate that the human chromosome region HSA3q13-q23 is likely to be orthologous to GGA15 and GGA14. The synteny breakpoint between the human and chicken gene segments was detected on HSA3q13.3-q23 between NL1-290 and the six other NotI clones. Previously available comparative mapping data suggest another breakpoint between all the above NotI clones and four genes, TFRC, EIF4A2, SKIL and DHX36, located on HSA3q24-qter and GGA9. Microchromosomal location of seven NotI clones from the HSA3q21 T-band region can be considered as an evidence in support of our hypothesis regarding the functional analogy of mammalian T-bands and avian microchromosomes
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