Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels

Mitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these parasites. As a model for understanding the co-ordination of these genomes, we have examined the regulation and mitochondrial import of a nuclear-encoded component of the cytochrome oxidase complex, cytochrome oxidase subunit VI (COXVI). By generating transgenic trypanosomes expressing intact or mutant forms of this protein, we demonstrate that COXVI is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of the protein are necessary for correct mitochondrial sorting. Analyses of endogenous and transgenic COXVI mRNA and protein expression in parasites undergoing developmental stage differentiation demonstrates a temporal order of control involving regulation in the abundance of, first, mRNA and then protein. This represents the first dissection of the regulation and import of a nuclear-encoded protein into the cytochrome oxidase complex in these organisms, which were among the earliest eukaryotes to possess a mitochondrion

Blueberry Progress Reports

The 1981 edition of the Blueberry Progress Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Life Sciences and Agriculture Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Weed Control in Lowbush Blueberry Fields 2. Pruning of Blueberries 3. 1982 Blueberry Fruit Fly Monitoring IPM Program 4. Physiology and Culture of the Lowbush Blueberry 5. Blueberry Diseases: Incidence and Control 6. Control, biology, and ecology of insects affecting lowbush blueberries 7. Blueberry Extension Progress Report 8. Plan of Work - 1982 - Blueberry Extensio

Blueberry Progress Reports

The 1980 edition of the Blueberry Progress Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Life Sciences and Agriculture Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Weed Control in Lowbush Blueberry Fields 2. Pruning of Blueberries 3. Integrated Pest Management of Blueberries in Maine 4. Physiology and Culture of the Lowbush Blueberry 5. Blueberry Diseases: Incidence and Control 6. Insects Affecting the Blueberry 7. Effect of Plant-Water Stress on Lowbush Blueberry Growth, Yield and Quality 8. Blueberry Extension Progress Report 9. Plan of Work -1981- Blueberry Extensio

Biogenesis of the mitochondrial phosphate carrier

The mitochondrial phosphate carrier (PiC) is a member of the family of inner-membrane carrier proteins which are generally synthesized without a cleavable presequence. Surprisingly, the cDNA sequences of bovine and rat PiC suggested the existence of an amino-terminal extension sequence in the precursor of PiC. By expressing PiC in vitro, we found that PiC is indeed synthesized as a larger precursor. This precursor was imported and proteolytically processed by mitochondria, whereby the correct amino-terminus of the mature protein was generated. Import of PiC showed the characteristics of mitochondrial protein uptake, such as dependence on ATP and a membrane potential and involvement of contact sites between mitochondrial outer and inner membranes. The precursor imported in vitro was correctly assembled into the functional form, demonstrating that the authentic import and assembly pathway of PiC was reconstituted when starting with the presequence-carrying precursor. These results are discussed in connection with the recently postulated role of PiC as an import receptor located in the outer membrane

Blueberry Advisory Committee Research Report

The 1985 edition of the Blueberry Progress Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Control of secondary blueberry pest insects 2. Effect of pruning practices on blueberry insect abundance 3. Effect of hexazinone on species distribution in lowbush blueberry fields 4. Dichlobenil for control of bunchberry 5. Evaluation of postemergence herbicides for grass control 6. Hand-wiper applications of herbicides on woody weeds 7. Evaluation of steam as a pruning practice for lowbush blueberry fields 8. Evaluation of glyphosate and 2,4-D applied with a commercial weed roller to control woody weeds 9. Hand-wiping and cutting treatments for dogbane 9. Evaluation of airblast-sprayer application of asulam for bracken fern control 10. Spot treatment of woody weeds with 2,4-D in oil 11. Chemical control of Botrytis blossom blight 12. Evaluation of preliminary steam treatments (1984) at Blueberry Hill Farm 13. Long term effects of N and NPK fertilizer on plant growth and yield 14. Nutritional survey of selected lowbush blueberry fields 15. Interaction of fertility and pruning practices on soil characteristics and lowbush blueberry growth and yield 16. Frequency of fertility application for establishment of lowbush blueberry seedlings 17. Slow release vs. liquid fertilizer for establishment of lowbush blueberry seedlings 18. The effect of several mulches on frost heaving, soil moisture, soil temperature and rhizome development 19. Blueberry product development: raisin-type blueberries 20. Blueberry product development: blueberry puree 21. Addendum: 1985 Report from Moody Trevet

Blueberry Research Progress Report

The 1976 edition of the Blueberry Research Progress Report was prepared for the Blueberry Advisory Committee by researchers at the University of Maine, Orono. Projects in this report include: 1. Effect of Plant-Water Stress on Lowbush Blueberry Growth, Yield and Quality 2. Insects Affecting the Blueberry 3. The Development of Blueberry Varieties Adapted to Maine 4. Weed Control in Blueberry Fields 5. Pruning of Blueberries 6. Integrated Management of Blueberry Fields 7. The Physiology and Biochemistry of the Development of the Lowbush Blueberry Fruit 8. Survey of Red Leaf Disease of Blueberries 9. Blossom Blight of Blueberries 10. Blueberry Marketing and Cost Analysis of Producing Blueberries 11. Factors Regulating Rhizome Initiation and Development in the Lowbush Blueberry 12. New Food Products and Servic

Biogenesis of mitochondrial porin

We review here the present knowledge about the pathway of import and assembly of porin into mitochondria and compare it to those of other mitochondrial proteins. Porin, like all outer mitochondrial membrane proteins studied so far is made as a precursor without a cleavble lsquosignalrsquo sequence; thus targeting information must reside in the mature sequence. At least part of this information appears to be located at the amino-terminal end of the molecule. Transport into mitochondria can occur post-translationally. In a first step, the porin precursor is specifically recognized on the mitochondrial surface by a protease sensitive receptor. In a second step, porin precursor inserts partially into the outer membrane. This step is mediated by a component of the import machinery common to the import pathways of precursor proteins destined for other mitochondrial subcompartments. Finally, porin is assembled to produce the functional oligomeric form of an integral membrane protein wich is characterized by its extreme protease resistance

Blueberry Advisory Committee Research Report

The 1988 edition of the Blueberry Advisory Committee Research Reports was prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Monitoring methods, economic injury levels, and action thresholds for blueberry spanworm larvae in vegetative year fields. 2. Control of secondary blueberry pests 3. Control of blueberry maggot 4. Effect of pruning practices on blueberry insect abundance 5. Survey of Fungi Contaminating Lowbush Blueberries 6. Nutrition Survey 1988 7. Phosphorus Dose/Response Curve 8. Effect of Several Mulches on Frost Heaving, Soil Moisture, Soil Temperature and Rhizome Development 9. Influence of Mulch Sources on Clonal Spread-SCS Study, Deblois 10. Effect of Surface Mulches on Stabilizing Lowbush Blueberry Soil in Barren Areas 11. Nitrogen-Phosphorus Study 12. Changes in Sugar and Organic Acids of Blueberries During Development, Preprocess Lag Time and Storage 13. Characterization of Pectin in Blueberries 14. Effect of Hexazinone (VELPAR) on Species Distribution in Lowbush Blueberry Fields 15. Evaluation of Setyhoxydim (POAST) for Bunchgrass Control 16. Evaluation and modification of commercial wipers 17. Evaluation of Five Preemergence Herbicides for Control of Oatgrass and Bunchgrass 18. Effect of rate and formulation of hexazinone (VELPAR) on bunchberry 19. Bracken fern control alternatives 20. Hexazinone (VELPAR) and terbacil (SINBAR) combinations for weed control 21. Evaluation of hexazinone (VELPAR) with spot treatments of glyphosate (ROUNDUP) or sethoxydin (POAST) for bunchgrass control 22. Directed sprays of glyphosate (ROUNDUP) for bunchberry control. 23. Evaluation of Postemergence Applications of Chlorimuron for Bunchberry Control 24. Seedling Pruning Study 25. Blueberry Harvester Trials 26. Blueberry Extension Progra

Blueberry Advisory Committee Research Report

The 1987 edition of the Blueberry Advisory Committee Research Reports was prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Effect of Pruning Practices on Blueberry Insect Abundance 2. Economic Thresholds and Control of Secondary Blueberry Pests 3. Control of Blueberry Maggot 4. Evaluation of Fungicides for Control of Botrytis Blight on Lowbush Blueberry, 1987 5. Evaluation of Fungicides for Control of Mummy Berry on Lowbush Blueberry, 1987 6. Miscellaneous Activity: a) Fungicide Residue Analysis b) Blueberry fact sheet c) Berry contamination 7. Effects of Pruning Methods on Mummy Berry Disease Incidence, 1987 8. Effects of Pruning Methods on Powdery Mildew and Red Leaf Disease Incidence, 1987 9. Nutrition Survey 1987 10. Nutritional Responses of Seedlings 11. The Effect of Several Mulches on Frost Heaving, Soil Moisture, Soil Temperature and Rhizome Development 12. Time-temperature Effects on Sugar Migration and Physical Characterization in Lowbush Blueberries 13. Fabricated Blueberry Raisins Prepared from Puree using Alginate as the Gelling Agent 14. Blueberry Gelatin 15. Effect of Hexazinone (VELPAR) on Species Distribution in Lowbush Blueberry Fields 16. Evaluation of Postemergence Herbicides for Grass Control 17. Evaluation of Sulfonyl urea and Imidazoline compounds for Bunchberry Control 18. Use of Mechanical wiper with glyphosate (ROUNDUP) or dicamba for control of dogbane 19. Integrated Weed Management 20. Evaluation of Five Preemergence Herbicides for Control of Oat grass and Bunchgrass 21. Wiper Application of Dicamba for Woody Weed control 22. Hexazinone (VELPAR) and terbacil (SINBAR) combinations for weed control 23. Evaluation of Clopyralid and Lactofen for Bunchberry Control 24. Evaluation of Postemergence Applications of chlorimuron for Bunchberry Control 25. Hexazinone seedling study 26. Seedling Pruning Study 27. Effect of bracken fern on blueberry yield 28. Evaluation of two mechanical harvesters vs hand raking of lowbush blueberrie

Blueberry Progress Reports

The 1978 edition of the Blueberry Progress Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Life Sciences and Agriculture Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Weed Control in Blueberry Fields 2. Pruning of Blueberries 3. Integrated Management of Blueberry Fields 4. Factors Regulating Rhizome Initiation and Development in the Lowbush Blueberry 5. Effect of Plant-Water Stress on Lowbush Blueberry Growth Yield and Quality 6. Blossom Blight of Blueberries 7. Botrytis Blossom Blight of Lowbush Blueberries 8. Insects Affecting the Blueberry 9. Treatment of Blueberries with Potassium Sorbate to Reduce Spoilage During Temporary Storage 10. Cooperative Extension Activitie