Comparación in vitro de filtración marginal entre ionómeros vítreos selladores

The aim of this study was to evaluate in vitro the marginal microleakage of two glass ionomer materials used as pit and fissure sealants. Thirty healthy premolars extracted for orthodontic treatment were randomly assigned to two groups (n=15) and respectively sealed with two glass ionomers (Group I, Fuji VII and Group II, Fuji IX). All teeth were preserved in artificial saliva (NAF) for 10 days, thermocycled (250 cycles; 5oC, 37oC and 60oC), isolated, and immersed in 2% alcohol gentian violet blue solution for 24 h. After washing, teeth were included in acrylic resin and sectioned longitudinally in a bucco-lingual direction with a Struers-Minitom cutting device. Samples were analyzed for leakage using an optical microscope (Olympus BX- 60M). TheWilliams and Winter semi-quantitative ranked scale was used to score dye penetration. In Group I the grades were distributedasfollows:Grade1,1sampleandGrade3,14samples (Mean 2.87 Median 3, SD 0.52). In Group II: Grade 0: 4 samples, Grade 1, 3 samples, Grade 2, 2 samples and Grade 3, 6 samples (Mean 1.67, Median 2, SD 1.29). Fisher’s exact test showed statistically significant differences between materials (p=0.006). From these results, we conclude that Fuji IX had better marginal sealing than Fuji VII when used as a pit and fissure sealant.El objetivo del trabajo fue comparar la eficacia como sellador de dos ionomeros vitreos. Se emplearon 30 premolares sanos extraidos por razones ortodoncicas. Se dividieron en dos grupos iguales (n=15) .Grupo I: Fuji VII y Grupo II: Fuji IX. Los dientes, fueron conservados en saliva artificial (NAF) durante 10 dias. Luego, fueron termociclados (250 ciclos) a 5oC, 37oC y 60oC. Posteriormente se sumergieron en violeta de genciana durante 24 hs. Una vez lavadas las muestras se seccionaron y evaluaron con el criterio de Williams y Winter, utilizando un microscopio optico (Olympus BX-60M) para valorar la penetracion del colorante. Los grados obtenidos para cada grupo fueron: en Grupo I (Fuji VII), Grado 1, 1 muestra y Grado 3, 14 muestras (Media 2,87, Mediana 3 y SD 0.52). En el Grupo II (Fuji IX) los grados se distribuyeron asi: Grado 0, 4 muestras, Grado 1, 3 muestras, Grado 2, 2 muestras y Grado 3, 6 muestras. (Media 1.67, Mediana 2 y SD 1.29). La Prueba exacta de Fisher (p=0,006) demostro diferencias estadisticamente significativa entre materiales. Se concluyo que el mejor sellado marginal fue obtenido utilizando el material Fuji IX comparado con Fuji VII cuando es utilizado como sellador.Fil: Sly, Gabriela Edith. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Nieva, Enrique Nicolás. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnologia; ArgentinaFil: Kaplan, Andrea Edith. Universidad de Buenos Aires. Facultad de Odontología; Argentin

Comparación in vitro de filtración marginal entre ionómeros vítreos como selladores de fisura

The aim of this study was evaluate in vitro the marginal microleakage of two glass ionomer materials used as pit-and-fissure sealants. Thirty healthy premolars, extracted for orthodontic treatment were randomly assigned in to II groups (n=15) and sealant by two glass ionomer (Group I: Fuji VII and Group II: Fuji IX). All teeth conserved in artificial saliva (NAF) for 10 days, were thermocycled (250 cycles; 5ºC, 37ºC and 60ºC), isolated, immersed in 2% alcohol solution gentian violet blue dye for 4 h. After washed, they were included in acrylic resin and sectioned longitudinally in buccolingual direction by cutting device (Miniton de Struers). Samples were analyzed for leakage using light microscope (Olympus BX-60M).  A semi-quantitative ranked scale from Williams & Winter was used to score dye penetration. Results: Group I was distributed as follows Grade 1: 1 and Grade 3: 14 (Mean 2.87 Median 3, SD 0.52). Group II was distributed: Grade 0: 4, Grade 1: 3 Grade 2: 2 and Grade 3: 6. (Mean 1.67, Median 2, SD 1.29). Fisher Exact Test showed statistically significant differences between materials (p = 0.006). From these results, we concluded that Fuji IX showed better marginal seal than Fuji VII, when used as pit- fissure sealant. The aim of this study was evaluate in vitro the marginal microleakage of two glass ionomer materials used as pit-and-fissure sealants. Thirty healthy premolars, extracted for orthodontic treatment were randomly assigned in to II groups (n=15) and sealant by two glass ionomer (Group I: Fuji VII and Group II: Fuji IX). All teeth conserved in artificial saliva (NAF) for 10 days, were thermocycled (250 cycles; 5ºC, 37ºC and 60ºC), isolated, immersed in 2% alcohol solution gentian violet blue dye for 4 h. After washed, they were included in acrylic resin and sectioned longitudinally in buccolingual direction by cutting device (Miniton de Struers). Samples were analyzed for leakage using light microscope (Olympus BX-60M).  A semi-quantitative ranked scale from Williams & Winter was used to score dye penetration. Results: Group I was distributed as follows Grade 1: 1 and Grade 3: 14 (Mean 2.87 Median 3, SD 0.52). Group II was distributed: Grade 0: 4, Grade 1: 3 Grade 2: 2 and Grade 3: 6. (Mean 1.67, Median 2, SD 1.29). Fisher Exact Test showed statistically significant differences between materials (p = 0.006). From these results, we concluded that Fuji IX showed better marginal seal than Fuji VII, when used as pit- fissure sealant. The aim of this study was evaluate in vitro the marginal microleakage of two glass ionomer materials used as pit-and-fissure sealants. Thirty healthy premolars, extracted for orthodontic treatment were randomly assigned in to II groups (n=15) and sealant by two glass ionomer (Group I: Fuji VII and Group II: Fuji IX). All teeth conserved in artificial saliva (NAF) for 10 days, were thermocycled (250 cycles; 5ºC, 37ºC and 60ºC), isolated, immersed in 2% alcohol solution gentian violet blue dye for 4 h. After washed, they were included in acrylic resin and sectioned longitudinally in buccolingual direction by cutting device (Miniton de Struers). Samples were analyzed for leakage using light microscope (Olympus BX-60M).  A semi-quantitative ranked scale from Williams & Winter was used to score dye penetration. Results: Group I was distributed as follows Grade 1: 1 and Grade 3: 14 (Mean 2.87 Median 3, SD 0.52). Group II was distributed: Grade 0: 4, Grade 1: 3 Grade 2: 2 and Grade 3: 6. (Mean 1.67, Median 2, SD 1.29). Fisher Exact Test showed statistically significant differences between materials (p = 0.006). From these results, we concluded that Fuji IX showed better marginal seal than Fuji VII, when used as pit- fissure sealant.Fil: Sly, Gabriela. Universidad Nacional de Tucuman. Facultad de Odontologia. Cat.de Anatomia Patologica/laboratorio de Patologia Molecular; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Nieva, Nadia Yanina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; ArgentinaFil: Kaplan, Andrea Edith. Universidad de Buenos Aires. Facultad de Odontología. Departamento de Materiales Dentales; ArgentinaXLIV Reunión Anual de la Sociedad Argentina de Investigación OdontológicaTucumánArgentinaSociedad Argentina de Investigación Odontológic

Emergence and Spread of Plasmid-Borne tet(B)::ISCR2 in Minocycline-Resistant Acinetobacter baumannii Isolates

Resistance to minocycline has emerged in multidrug-resistant Acinetobacter baumannii isolates from Buenos Aires Hospitals. Few reports about the description and dispersion of tet genes were published in this species. We observed the presence of tet(B) in all minocycline resistant isolates. This gene was found associated to the ISCR2 mobile element, which could in part explain its dispersion.Fil: Vilacoba, Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Cátedra de Microbiología, Parasitología e Inmunología; ArgentinaFil: Almuzara, Marisa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos;Fil: Gulone, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; ArgentinaFil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Figueroa, Silvia A.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos;Fil: Sly, Gabriela Edith. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos;Fil: Fernandez, Analia. Universidad de Buenos Aires; Argentina. Universidad Favaloro; ArgentinaFil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentin

Evaluación clínica de cementos de ionómero vítreo aplicados como selladores de fosas y fisuras

The aim of this study was to evaluate the clinical behavior of two conventional glass ionomers used for pit and fissure sealing in terms of retention, marginal adaptation, caries recurrence and cracking. Eighty-three fully erupted first permanent molars were sealed, in a group of children aged 5 to 8 years. A doubleblind, single operator, paired design was used. Materials applied were Fuji IX and VII. Retention (R), presence of caries (PC), marginal discoloration (MD), marginal adaptation (MA) and cracking (C) were evaluated at 6 and 12 months using Ryge’s criteria. Data registered 6 months after treatment were: Fuji IX: R: Alpha 37, Bravo 2, Charlie: 4; PC: Alpha 43, Bravo: 0; MA: Alpha 38, Bravo: 1; MD: Alpha 39, Bravo: 0, Charlie: 0; C: Alpha 39, Bravo: 0. Fuji VII: R: Alpha 29, Bravo 4, Charlie: 7; PC: Alpha 40, Bravo: 0; MA: Alpha: 34, Bravo: 0; MD: Alpha: 34, Bravo: 0; Charlie: 0; C: Alpha 33, Bravo: 0. Results after one year were: Fuji IX: R: Alpha 33, Bravo: 3, Charlie: 9; PC: Alpha 44, Bravo: 1; MA: Alpha 33, Bravo: 1; MD: Alpha: 34, Bravo: 0, Charlie: 0; C: Alpha: 34, Bravo: 0. Fuji VII: R: Alpha 22, Bravo 4, Charlie: 13, PC: Alpha 40, Bravo: 0; MA: Alpha 23, Bravo: 1; MD: Alpha 23, Bravo: 1, Charlie: 0; C: Alpha 23, Bravo: 1. Statistical analysis using Fisher test showed no significant difference (p>0.05) for R, and MA. For PC, MD and C, values are not reported because both materials showed the same results. The second control showed no significant difference (p>0.05) for R, MA, PC, MD and C. Results suggest no difference between Fuji IX and Fuji VII as sealants in fully erupted permanent molars.El objetivo de este trabajo fue evaluar el comportamiento clinico de dos ionomeros vitreos convencionales aplicados como selladores de fosas y fisuras. Se evaluo la retencion, decoloracion y adaptacion marginal, incidencia de caries y agrietamiento. Se sellaron ochenta y tres primeros molares totalmente erupcionados en un grupo de ninos de 5 a 8 anos. Se llevo a cabo un diseno de doble ciego, unico operador y apareado. Los materiales aplicados fueron Fuji IX y VII. Luego de 6 y 12 meses se recitaron los ninos y se evaluo: Retencion (R), presencia de caries (PC), decoloracion marginal (MD) y agrietamiento (C). La evaluacion se realizo utilizando los criterios de Ryge. Los datos registrados al cabo de 6 meses fueron: Fuji IX: R: Alpha 37, Bravo 2, Charlie: 4; PC: Alpha 43, Bravo: 0; MA: Alpha 38, Bravo: 1; MD: Alpha 39, Bravo: 0, Charlie: 0; C: Alpha 39, Bravo: 0. Fuji VII: R: Alpha 29, Bravo 4, Charlie: 7; PC: Alpha 40, Bravo: 0; MA: Alpha: 34, Bravo: 0; MD: Alpha: 34, Bravo: 0; Charlie: 0; C: Alpha 33, Bravo: 0. A los 12 meses: Fuji IX: R: Alpha 33, Bravo: 3, Charlie: 9; PC: Alpha 44, Bravo: 1; MA: Alpha 33, Bravo: 1; MD: Alpha: 34, Bravo: 0, Charlie: 0; C: Alpha: 34, Bravo: 0. Fuji VII: R: Alpha 22, Bravo 4, Charlie: 13, PC: Alpha 40, Bravo: 0; MA: Alpha 23, Bravo: 1; MD: Alpha 23, Bravo: 1, Charlie: 0; C: Alpha 23, Bravo: 1. La evaluacion estadistica por medio de la prueba de Fisher no mostro diferencias significativas entre los materiales (p>0.05) para R y MA. En PC, MD y C, el valor de p no se indica ya que los resultados fueron identicos. El segundo control no mostro diferencias significativas (p>0.05) para R, MA, PC, MD y C. Los resultados sugieren que no existiria diferencia en la aplicacion de Fuji IX y Fuji VII como selladores de fosas y fisuras en molares totalmente erupcionados.Fil: Sly, Edith Gabriela. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Kaplan, Andrea Edith. Universidad de Buenos Aires; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán; Argentin