29 research outputs found
Susceptibility of Anopheles campestris-like and Anopheles barbirostris species complexes to Plasmodium falciparum and Plasmodium vivax in Thailand
Simultaneous bilateral triquetral fractures, acquired in two separate accidents within 9 h
Effects of elevated haemolymph levels of the female gonadotropic dorsal body hormone on the cercarial production ofTrichobilharzia ocellata inLymnaea stagnalis
Increased activity of the female gonadotropic hormone producing Dorsal Bodies inLymnaea stagnalis infected withTrichobilharzia ocellata
Detection of antigen in sera of patients with invasive aspergillosis:Intra- and interlaboratory reproducibility
Detection of antigen in sera of patients with invasive aspergillosis:Intra- and interlaboratory reproducibility
Detection of antigen in sera of patients with invasive aspergillosis:Intra- and interlaboratory reproducibility
The intra-and interlaboratory reproducibilities of a commercial sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Aspergillus galactomannan in serum (Platelia Aspergillus; Sanofi Diagnostics Pasteur, Marnes-La-Coquette, France) were evaluated in six laboratories of university hospitals. Twenty serum samples mere obtained from 12 neutropenic patients including 6 with invasive aspergillosis. These samples were blinded and sent to each center together with eight blinded ELISA-negative serum samples spiked with known concentrations of galactomannan. The centers were provided with ELISA microtiter plates from a single batch and a detailed protocol. Ten clinical samples showed ELISA reactivity, while 10 samples were ELISA negative. The mean coefficient of variation (CV) of the optical density values was 4.24% within a single assay and 25.6% between runs. The interassay CV of the ratios for the serum samples tested was 18.6%. Analysis of ordinal interpretation of the ELISA result (i.e., negative, gray zone, or positive) showed excellent reproducibility. Recalculation of the cutoff values for positive and negative samples suggested that the cutoff level recommended by the manufacturer could be lowered from 1.0 to 0.8 for negative samples and from 1.5 to 1.0 for positive samples. The intra-and interlaboratory reproducibilities were excellent when the ELISA results were interpreted as ordinal data, but considerable variation in optical density values and, to a lesser extent, in the ratios for the serum samples tested, was observed between runs. High assay variability was also found for serum samples spiked with known concentrations of galactomannan, Therefore, antigen titers in serum samples from a single patient, measured in different runs, should be compared with caution.</p
Detection of Antigen in Sera of Patients with Invasive Aspergillosis: Intra- and Interlaboratory Reproducibility
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