Probe-pulse optimization for nonresonant suppression in hybrid fs/ps coherent anti-Stokes Raman scattering at high temperature

Hybrid femtosecond/picosecond coherent anti-Stokes Raman scattering (fs/ps CARS) offers accurate thermometry at kHz rates for combustion diagnostics. In high-temperature flames, selection of probe-pulse characteristics is key to simultaneously optimizing signal-to-nonresonant-background ratio, signal strength, and spectral resolution. We demonstrate a simple method for enhancing signal-to-nonresonant-background ratio by using a narrowband Lorentzian filter to generate a time-asymmetric probe pulse with full-width-half-maximum (FWHM) pulse width of only 240 fs. This allows detection within just 310 fs after the Raman excitation for eliminating nonresonant background while retaining 45% of the resonant signal at 2000 K. The narrow linewidth is comparable to that of a time-symmetric sinc2 probe pulse with a pulse width of ~2.4 ps generated with a conventional 4-f pulse shaper. This allows nonresonant-background-free, frequency-domain vibrational spectroscopy at high temperature, as verified using comparisons to a time-dependent theoretical fs/ps CARS model

Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood

We report a transient absorption (TA) imaging method for fast visualization and quantitative layer analysis of graphene and GO. Forward and backward imaging of graphene on various substrates under ambient condition was imaged with a speed of 2 μs per pixel. The TA intensity linearly increased with the layer number of graphene. Real-time TA imaging of GO in vitro with capability of quantitative analysis of intracellular concentration and ex vivo in circulating blood were demonstrated. These results suggest that TA microscopy is a valid tool for the study of graphene based materials

Optimization of a High-Speed X-Ray Imaging System for Studying Sprays

Spray-based liquid atomization and liquid mixing is critical for development of efficient combustors and drug delivery systems as well as multiple coating-related applications. While optical methods allow characterization of low density regions of sprays, the scattering of optical photons hinders the characterization of a dense core. Unlike optical photons, higher-energy X-ray photons have the capability to penetrate and image the core structure of sprays. Here we characterized temporal and spatial resolution of an X-Ray imaging system based on a commercially available tube source with an anode size of 0.6 mm. For high-speed imaging, a phosphor screen in combination with a high-speed CMOS camera equipped with a two-stage intensifier was used. Water was used as a model liquid with the addition of potassium iodide to increase the X-Ray absorption coefficient. Two-dimensional images of 0.5mm and 2 mm impinging jet sprays were taken with differing spatial resolutions and potassium iodide mass concentrations. Depending on the spray conditions, optimal imaging settings were found. The technique can be extended to three-dimensional analysis of sprays with multiple viewing angles from two or more X-ray sources along with tomographic reconstruction

Intense pulsed helium droplet beams

Pulsed (30 - 100 microseconds) nozzle beams have been used to generate helium droplets ( = 10^4-10^5). The dependence of the beam intensity and the mean droplet size on the source stagnation pressure and temperature are studied via mass spectroscopy and laser induced fluorescence of embedded phthalocyanine molecules. In comparison to a cw beam the pulsed source for the same pressure and temperature has a factor of 100 higher flux and the droplet sizes are an order of a magnitude larger.Comment: Accepted by Review of Scientific Instrument

All-diode-pumped quasi-continuous burst-mode laser for extended high-speed planar imaging

An all-diode-pumped, multistage Nd:YAG amplifier is investigated as a means of extending the duration of high-power, burst-mode laser pulse sequences to an unprecedented 30 ms or more. The laser generates 120 mJ per pulse at 1064.3 nm with a repetition rate of 10 kHz, which is sufficient for a wide range of planar laser diagnostics based on fluorescence, Raman scattering, and Rayleigh scattering, among others. The utility of the technique is evaluated for image sequences of formaldehyde fluorescence in a lifted methane–air diffusion flame. The advantages and limitations of diode pumping are discussed, along with long-pulse diode-bar performance characteristics to guide future designs

Hybrid femtosecond/picosecond coherent anti-Stokes Raman scattering for high-speed gas-phase thermometry

We demonstrate hybrid femtosecond/picosecond (fs/ps) coherent anti-Stokes Raman scattering for high-speed thermometry in unsteady high-temperature flames, including successful comparisons with a time- and frequencyresolved theoretical model. After excitation of the N2 vibrational manifold with 100 fs broadband pump and Stokes beams, the Raman coherence is probed using a frequency-narrowed 2:5 ps probe beam that is time delayed to suppress the nonresonant background by 2 orders of magnitude. Experimental spectra were obtained at 500 Hz in steady and pulsed H2–air flames and exhibit a temperature precision of 2.2% and an accuracy of 3.3% up to 2400 K. Strategies for real-time gas-phase thermometry in high-temperature flames are also discussed, along with implications for kilohertz-rate measurements in practical combustion systems

Biaxial deformation of collagen and elastin fibers in coronary adventitia

The microstructural deformation-mechanical loading relation of the blood vessel wall is essential for understanding the overall mechanical behavior of vascular tissue in health and disease. We employed simultaneous mechanical loading-imaging to quantify in situ deformation of individual collagen and elastin fibers on unstained fresh porcine coronary adventitia under a combination of vessel inflation and axial extension loading. Specifically, the specimens were imaged under biaxial loads to study microscopic deformation-loading behavior of fibers in conjunction with morphometric measurements at the zero-stress state. Collagen fibers largely orientate in the longitudinal direction, while elastin fibers have major orientation parallel to collagen, but with additional orientation angles in each sublayer of the adventitia. With an increase of biaxial load, collagen fibers were uniformly stretched to the loading direction, while elastin fibers gradually formed a network in sublayers, which strongly depended on the initial arrangement. The waviness of collagen decreased more rapidly at a circumferential stretch ratio of λθ = 1.0 than at λθ = 1.5, while most collagen became straightened at λθ = 1.8. These microscopic deformations imply that the longitudinally stiffer adventitia is a direct result of initial fiber alignment, and the overall mechanical behavior of the tissue is highly dependent on the corresponding microscopic deformation of fibers. The microstructural deformation-loading relation will serve as a foundation for micromechanical models of the vessel wall

Quasi-continuous burst-mode laser for high-speed planar imaging

The pulse-burst duration of a compact burst-mode Nd:YAG laser is extended by one order of magnitude compared to previous flashlamp-pumped designs by incorporating a fiber oscillator and diode-pumped solid-state amplifiers. The laser has a linewidth of \u3c2 GHz at 1064.3 nm with 150 mJ per individual pulse at 10 kHz. The performance of the system is evaluated by using the third-harmonic output at 354.8 nm for high-speed planar laser-induced fluorescence of formaldehyde in a lifted methane-air diffusion flame. A total of 100 and 200 sequential images of unsteady fluid-flame interactions are acquired at repetition rates of 10 kHz and 20 kHz, respectively