4 research outputs found
Assessment of adipokines, adenine nucleotides and uric acid in the dynamics of coronary intervention
Introduction: The association of vaspin
and visfatin, with a myocardial infarction is still
not fully understood. Reduced levels of adenine
nucleotides are hallmarks of chronic heart failure.
There is little data concerning the relationship
between these markers and their changes over time.
Material/Methods: The concentration of adenine
nucleotides, vaspin and visfatinwere assessed in 41
consecutive patients with acute myocardial infarction one
before (day I) and four days after (day IV) percutaneous
coronary intervention (PCI) and a control group.
Results: Visfatin concentrations were higher before and
after PCI vs. control (visfatin I: median 25.55, 20.12 - 30.69
ng/ml; visfatin IV: median 20.79, 16.89 - 25.61 ng/ml vs.
control: median 14.94, 10.66 - 25.25 ng/ml; p < 0.0001).
Vaspin concentrations were lower before and after PCI vs.
control (vaspin I: median 0.18, 0.11 - 0.44 ng/ml; vaspin IV:
median 0.24, 0.15 - 0.58 ng/ml vs. control: median 1.303,
1.13 - 2.26 ng/ml, p < 0.00001). Concentrations of visfatin, day I, correlated well to vaspin concentrations (r2 = 0.201,
p = 0.011). ATP levels were significantly lower in patients
vs. controls (day I: p = 0.00012; day IV: p = 0.0001).
Conclusions: Changes in the analyzed visfatin and vaspin
concentrations can be used as potential MI markers.
Visfatin serum concentration may be considered a
potential marker to differentiate MI over time
Assessment of adipokines, adenine nucleotides and uric acid in the dynamics of coronary intervention
Introduction: The association of vaspin
and visfatin, with a myocardial infarction is still
not fully understood. Reduced levels of adenine
nucleotides are hallmarks of chronic heart failure.
There is little data concerning the relationship
between these markers and their changes over time.
Material/Methods: The concentration of adenine
nucleotides, vaspin and visfatinwere assessed in 41
consecutive patients with acute myocardial infarction one
before (day I) and four days after (day IV) percutaneous
coronary intervention (PCI) and a control group.
Results: Visfatin concentrations were higher before and
after PCI vs. control (visfatin I: median 25.55, 20.12 - 30.69
ng/ml; visfatin IV: median 20.79, 16.89 - 25.61 ng/ml vs.
control: median 14.94, 10.66 - 25.25 ng/ml; p < 0.0001).
Vaspin concentrations were lower before and after PCI vs.
control (vaspin I: median 0.18, 0.11 - 0.44 ng/ml; vaspin IV:
median 0.24, 0.15 - 0.58 ng/ml vs. control: median 1.303,
1.13 - 2.26 ng/ml, p < 0.00001). Concentrations of visfatin, day I, correlated well to vaspin concentrations (r2 = 0.201,
p = 0.011). ATP levels were significantly lower in patients
vs. controls (day I: p = 0.00012; day IV: p = 0.0001).
Conclusions: Changes in the analyzed visfatin and vaspin
concentrations can be used as potential MI markers.
Visfatin serum concentration may be considered a
potential marker to differentiate MI over time
Metabolic surgery in Zucker rats influenced miRNA, caveolin-1 expression and lipid metabolism
Aims: A transposition of the long segment of
distal ileum in obese Zucker rats improved glucose tolerance
6 months after IT. It was undertaken to compare the gene
expression of miRNA-103, -107 and caveolin-1 in the liver
of euglycemic groups of IT relative to SHAM operated rats.
Main methods: Obese, male Zucker rats underwent
either transposition of 50% distal ileum or sham
surgery. For determining the gene expression, the
Real-Time PCR for caveolin-1 and miRNA-103, -107 was
performed. Plasma concentrations of LDL, HDL, TG
and total cholesterol were measured with enzymatic
colorimetric assays after optimization procedure.
Key findings: The Cav-1 expression in liver tissue after
ileal transposition was 1.22 times higher compared to the
SHAM group (SHAM median 63.58, min 41.3, max 82.4; IT
median 77.35, min 60.8, max 95.41, p < 0.001). miRNA-107
expression was significantly downregulated by 0.6-fold in
the IT group compared to the SHAM group (SHAM median
507.51, min 236.42, max 721.29; IT median 355.2, min 278.15, max 478.15, p < 0.015. The level of TG was significantly
higher after IT surgery (SHAM median 115, min 96, max
143; IT median 153, min 115, max 162, p = 0.001). The
total cholesterol plasma levels decreased after IT (SHAM
median 178, min 161, max 183; IT median 128, min 103,
max 114, p < 0.000001). The LDL plasma level in IT was
two-fold lower than in the SHAM (SHAM median 117, min
68, max 151; IT median 58, min 45, max 61, p < 0.000001).
Significance: The transposition of 50% of the distal ileum
lead to an increase in caveolin-1 and reduction in miR-107
expression compared to those of SHAM group. Endogenous
miR-107 is more involved in regulation of the functions of
insulin-target liver tissue than miRNA-103. Reduced LDL
and cholesterol plasma levels suggest positive effects on
lipid metabolism in long-term observations. The present
study is the first to show a lack of IT effect regarding
triglycerides six months after surgery