2,184 research outputs found
One Dimensional ary Density Classification Using Two Cellular Automaton Rules
Suppose each site on a one-dimensional chain with periodic boundary condition
may take on any one of the states , can you find out the most
frequently occurring state using cellular automaton? Here, we prove that while
the above density classification task cannot be resolved by a single cellular
automaton, this task can be performed efficiently by applying two cellular
automaton rules in succession.Comment: Revtex, 4 pages, uses amsfont
Finding The Sign Of A Function Value By Binary Cellular Automaton
Given a continuous function , suppose that the sign of only has
finitely many discontinuous points in the interval . We show how to use
a sequence of one dimensional deterministic binary cellular automata to
determine the sign of where is the (number) density of 1s in
an arbitrarily given bit string of finite length provided that satisfies
certain technical conditions.Comment: Revtex, uses amsfonts, 10 page
Knockdown of piRNA pathway proteins results in enhanced Semliki forest virus production in mosquito cells
The exogenous siRNA pathway is important in restricting arbovirus infection in mosquitoes. Less is known about the role of the PIWI-interacting RNA pathway, or piRNA pathway, in antiviral responses. Viral piRNA-like molecules have recently been described following infection of mosquitoes and derived cell lines with several arboviruses. The piRNA pathway has thus been suggested to function as an additional small RNA-mediated antiviral response to the known infection-induced siRNA response. Here we show that piRNA-like molecules are produced following infection with the naturally mosquito-borne Semliki Forest virus in mosquito cell lines. We show that knockdown of piRNA pathway proteins enhances the replication of this arbovirus and defines the contribution of piRNA pathway effectors, thus characterizing the antiviral properties of the piRNA pathway. In conclusion, arbovirus infection can trigger the piRNA pathway in mosquito cells, and knockdown of piRNA proteins enhances virus production
Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics
Myogenesis, the formation of skeletal muscle, is a multistep event that commences with myoblast proliferation, followed by cell-cycle arrest, and finally the formation of multinucleated myotubes via fusion of mononucleated myoblasts. Each step is orchestrated by well-documented intracellular factors, such as cytoplasmic signalling molecules and nuclear transcription factors. Regardless, the key step in getting a more comprehensive understanding of the regulation of myogenesis is to explore the extracellular factors that are capable of eliciting the downstream intracellular factors. This could further provide valuable insight into the acute cellular response to extrinsic cues in maintaining normal muscle development. In this paper, we survey the intracellular factors that respond to extracellular cues that are responsible for the cascades of events during myogenesis: myoblast proliferation, cell-cycle arrest of myoblasts, and differentiation of myoblasts into myotubes. This focus on extracellular perspective of muscle development illustrates our mass spectrometry-based proteomic approaches to identify differentially expressed secreted factors during skeletal myogenesis
Computer simulations of electrorheological fluids in the dipole-induced dipole model
We have employed the multiple image method to compute the interparticle force
for a polydisperse electrorheological (ER) fluid in which the suspended
particles can have various sizes and different permittivites. The point-dipole
(PD) approximation being routinely adopted in computer simulation of ER fluids
is shown to err considerably when the particles approach and finally touch due
to multipolar interactions. The PD approximation becomes even worse when the
dielectric contrast between the particles and the host medium is large. From
the results, we show that the dipole-induced-dipole (DID) model yields very
good agreements with the multiple image results for a wide range of dielectric
contrasts and polydispersity. As an illustration, we have employed the DID
model to simulate the athermal aggregation of particles in ER fluids both in
uniaxial and rotating fields. We find that the aggregation time is
significantly reduced. The DID model accounts for multipolar interaction
partially and is simple to use in computer simulation of ER fluids.Comment: 22 pages, 7 figures, submitted to Phys. Rev.
Guggulsterone Targets Smokeless Tobacco Induced PI3K/Akt Pathway in Head and Neck Cancer Cells
Epidemiological association of head and neck cancer with smokeless tobacco (ST) emphasizes the need to unravel the molecular mechanisms implicated in cancer development, and identify pharmacologically safe agents for early intervention and prevention of disease recurrence. Guggulsterone (GS), a biosafe nutraceutical, inhibits the PI3K/Akt pathway that plays a critical role in HNSCC development. However, the potential of GS to suppress ST and nicotine (major component of ST) induced HNSCC remains unexplored. We hypothesized GS can abrogate the effects of ST and nicotine on apoptosis in HNSCC cells, in part by activation of PI3K/Akt pathway and its downstream targets, Bax and Bad.Our results showed ST and nicotine treatment resulted in activation of PI3K, PDK1, Akt, and its downstream proteins--Raf, GSK3β and pS6 while GS induced a time dependent decrease in activation of PI3K/Akt pathway. ST and nicotine treatment also resulted in induction of Bad and Bax phosphorylation, increased the association of Bad with 14-3-3ζresulting in its sequestration in the cytoplasm of head and neck cancer cells, thus blocking its pro-apoptotic function. Notably, GS pre-treatment inhibited ST/nicotine induced activation of PI3K/Akt pathway, and inhibited the Akt mediated phosphorylation of Bax and Bad.In conclusion, GS treatment not only inhibited proliferation, but also induced apoptosis by abrogating the effects of ST/nicotine on PI3K/Akt pathway in head and neck cancer cells. These findings provide a rationale for designing future studies to evaluate the chemopreventive potential of GS in ST/nicotine associated head and neck cancer
Cohomology of bundles on homological Hopf manifold
We discuss the properties of complex manifolds having rational homology of
including those constructed by Hopf, Kodaira and
Brieskorn-van de Ven. We extend certain previously known vanishing properties
of cohomology of bundles on such manifolds.As an application we consider
degeneration of Hodge-deRham spectral sequence in this non Kahler setting.Comment: To appear in Proceedings of 2007 conference on Several complex
variables and Complex Geometry. Xiamen. Chin
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