2 research outputs found
Prevalence and zoonotic potential of canine hookworms in Malaysia
Background: Canine hookworm infection is endemic in Southeast Asian countries with a prevalence ranging from
70% to 100%, with zoonotic transmission representing a potentially significant public health concern. However,
there are limited data available on the prevalence of canine hookworms in Malaysia. This study was conducted to
determine the prevalence of hookworm and Ancylostoma species among dogs in Malaysia.
Methods: Faecal samples were collected from 221 dogs living in urban areas, rural areas and animal shelters in
Selangor. Faecal samples were processed using the formal-ether concentration technique followed by wet mount
preparation and iodine staining for the detection of hookworm eggs. Samples positive for hookworm eggs were
examined using PCR, targeting ITS2 and 28 s rRNA region, and subsequently sequenced in both directions. The
sequences were phylogenetically analysed using MrBayes for Bayesian Inference.
Results: The overall prevalence of hookworm among dogs was 48% (95%CI; 41.41β54.95). Rural stray dogs had the
highest prevalence 71.4% (95%CI; 61.13β81.49) followed by urban stray dogs, recording 48% (95%CI; 34.15β61.85)
and lastly dogs in shelters with 28.7% (95%CI; 19.56β37.84). Logistic regression identified rural stray dogs as a high
risk group (OR = 4.55, 95%; 2.50β8.31) and keeping dogs in shelters as a protective factor (OR = 0.24, 95%; 0.14β0.43).
Molecular methods identified both Ancylostoma ceylanicum and Ancylostoma caninum with A. ceylanicum being
predominant among urban stray dogs. Rural dogs had a higher prevalence of A. caninum than A. ceylanicum, while
both species showed equal distribution among dogs in shelters. Phylogenetic analysis placed A. ceylanicum isolated from dogs in one group with A. ceylanicum human isolates.
Conclusion: This study indicates that dogs have the potential to act as reservoir hosts of human hookworm infection in Malaysia. This finding necessitates the inclusion of dogs in any interventions to combat hookworm in the country
Aplikasi Video Parallax Menerusi Karya Fotografi "Miniatur"
This study aims to investigate the method of effective production through a miniature photography approach to food subjects. By identifying and analyzing the methods
used by the artist's reference artist to process the work through the artist's references that have been made. Successful methods will be used as a miniature photographic work through the selection of appropriate food subjects. The images that have been successfully produced will be edited into a parallax video presentation for the audience to see. The question and research questions will be investigated by the
researcher to obtain information about the research to be done later