Human cytomegalovirus encodes an MHC class I-like molecule (UL142) that functions to inhibit NK cell lysis

Clinical and low passage strains of human CMV (HCMV) encode an additional MHC class I-related molecule UL142, in addition to the previously described UL18. The UL142 open reading frame is encoded within the ULb' region which is missing from a number of common high passage laboratory strains. Cells expressing UL142 following transfection, and fibroblasts infected with a recombinant adenovirus-expressing UL142, were used to screen both polyclonal NK cells and NK cell clones, in a completely autologous system. Analysis of 100 NK cell clones derived from five donors, revealed 23 clones that were inhibited by fibroblasts expressing UL142 alone. Small-interfering RNA-mediated knockdown of UL142 mRNA expression in HCMV-infected cells resulted in increased sensitivity to lysis. From these data we conclude that UL142 is a novel HCMV-encoded MHC class I-related molecule which inhibits NK cell killing in a clonally dependent manner

Desempenho de bezerros da raça Holandesa alimentados com proteína de soja sólida ou líquida Performance of Holstein calves fed soybean meal protein in solid or liquid form

Avaliaram-se os efeitos da substituição de metade do total de proteína da dieta na forma sólida (farelo de soja) por proteína na forma líquida (leite de soja), com relações sólidos:líquidos de 100,0:0%; 87,5:12,5%; e 75,0:25,0%. Utilizaram-se 24 bezerros machos da raça Holandesa com 60 dias de idade, distribuídos em delineamento em blocos ao acaso, com três dietas à base de concentrado (80%) e feno de capim coast-cross (Cynodon dactylon) (20%), balanceadas para nitrogênio e energia. Nas dietas com partes líquidas, o intuito foi manter o reflexo de formação da goteira esofagiana no período experimental de 10 meses, no qual os animais foram mantidos em confinamento, sendo abatidos ao atingirem pesos corporais superiores a 400 kg. Em alguns dos animais, dotados de cânulas de rúmen, foi possível detectar neste órgão apenas pequenas quantidades do leite de soja ingerido, confirmando sucesso na formação da goteira esofagiana. Os ganhos de peso diários apresentaram redução linear (1,399; 1,341; e 1,191 kg) à medida que foram fornecidas maiores quantidades de leite de soja. As conversões de matéria seca e proteína bruta em ganhos de peso, durante os últimos 60 dias experimentais, pioraram conforme aumentaram as quantidades de proteína na forma líquida. O fornecimento de proteína na forma líquida na dieta não altera os rendimentos de carcaça em bezerros holandeses.<br>The effects were assessed of substituting half the total diet protein in solid form (soybean meal) with liquid form (soybean milk) at solid:liquid ratios of: 100.0:0% L; 87.5:12.5% L; 75.0:25.0% L. Twenty-four Holstein breed 60-d male calf steers were assigned to a randomized block design, with three concentrate (80%) diets and 20% coast-cross (Cynodon dactylon) hay, balanced for nitrogen and energy. In the diets with liquid parts, the intention was to keep the functional reflex of the esophageal groove throughout the 10 months of the experimental period, in which the animals were kept in a feedlot until slaughter at body weights higher than 400 kg. In some of the animals, with rumen canulas, only small quantities of ingested soybean milk were detected in the rumen contents, indicating success in esophageal groove formation. Daily weight gains were linearly reduced (1.399, 1.341 and 1.191 kg) as the treatments supplied larger quantities of soybean milk. Dry matter and crude protein conversions in weight gains, in the last 60 days of the experimental period, declined as the proportions of liquid feed increased. Supplying protein in liquid form in the diet did not alter carcass yield in Holstein steer