Correlation of HCMV UL144 genotypes with clinical indicators of hepatic involvement in neonates.

<p>Correlation of HCMV UL144 genotypes with clinical indicators of hepatic involvement in neonates.</p

Polymorphisms and features of cytomegalovirus UL144 and UL146 in congenitally infected neonates with hepatic involvement

<div><p>Human cytomegalovirus is a significant agent of hepatic involvement in neonates. In this study, we investigated the polymorphisms and features of the viral genes UL144 and UL146 as well as their significance to congenital hepatic involvement. In 79 neonates with congenital cytomegalovirus infection and hepatic involvement, full length UL144 and UL146 were successfully amplified in 73.42% and 60.76% of cases, respectively. Sequencing indicated that both genes were hypervariable. Notably, UL144 genotype B was highly associated with aspartate aminotransferase (<i>P</i> = 0.028) and lactate dehydrogenase (<i>P</i> = 0.046). Similarly, UL146 genotype G1 and G13 were significantly associated with CMV IgM (<i>P</i> = 0.026), CMV IgG (<i>P</i> = 0.034), alanine aminotransferase (<i>P</i> = 0.019), and aspartate aminotransferase (<i>P</i> = 0.032). In conclusion, dominant UL144 (genotype B) and UL146 (genotype G1 and G13) genotypes are associated with elevated levels of enzymes and CMV IgM and IgG of cytomegalovirus infection.</p></div

Alignment of amino acid sequences and comparison of post-translational modification motifs in UL144.

<p>UL144 amino acid sequences, along with representative sequences of genotypes A, B, and C (GenBank accession numbers AF498086-AF498088) as reference. Cysteine-rich domains (CRD), transmembrane region (tm), and cytoplasmic tail (cyto) are marked.</p

UL144 and UL146 genes in patients with congenital cytomegalovirus hepatic involvement.

<p>(A, B) PCR detection and identification of human cytomegalovirus (HCMV) UL144 and UL146 in urinary sediment, with expected size of 740 bp and 721 bp, respectively. M, 100 bp DNA marker; (A) Lane 1–9, UL144, from the sample 2H, 5H, 6H, 10H, 12H, 14H, 22H, 23H, and 25H, respectively; Lane 10, negative control (NC, HCMV negative urine sample); (B) Lane 1–6, UL146, from the sample 58H, 59H, 60H, 61H, 62H, and 63H, respectively; Lane 7, negative control (NC, HCMV negative urine sample). (C, D) PCR Sensitivity. UL144 and UL146 were amplified by PCR, and separated on 1.5% agarose. Lane 1–5, reactions with 10⁴, 10³, 10², 10¹, and 10⁰ copies, respectively; M, D2000 DNA marker.</p

Genotype distribution of HCMV UL146 in isolates from different cohorts and geographic regions.

<p>Genotype distribution of HCMV UL146 in isolates from different cohorts and geographic regions.</p

Functional motifs in the HCMV UL144 protein from different UL144 genotypes.

<p>Functional motifs in the HCMV UL144 protein from different UL144 genotypes.</p

Primers and reaction conditions for amplifying full length viral ORFs.

<p>Primers and reaction conditions for amplifying full length viral ORFs.</p

Functional motifs in the HCMV UL146 protein from different UL146 genotypes.

<p>Functional motifs in the HCMV UL146 protein from different UL146 genotypes.</p

The frequency of UL144 and UL146 and mixed infections in neonates with congenital cytomegalovirus hepatic involvement.

<p>The frequency of UL144 and UL146 and mixed infections in neonates with congenital cytomegalovirus hepatic involvement.</p

Correlation of the HCMV UL146 genotypes with clinical indicators of hepatic involvement in neonates.

<p>Correlation of the HCMV UL146 genotypes with clinical indicators of hepatic involvement in neonates.</p