2 research outputs found
Antimalarial and cytotoxic constituents of <i>Xylaria</i> cf. <i>cubensis</i> PK108
<p><i>Xylaria</i> cf. <i>cubensis</i> PK108 was identified by its distinctive morphological characteristics and its internal transcribed spacers sequence analysis. The chromatographic separation and structural elucidation based on spectroscopic analysis of fungal crude extracts led to 10 compounds; tryptoquivaline L (<b>1</b>), fiscalin C (<b>2</b>), <i>epi</i>-fiscalin C (<b>3</b>), cytochalasin D (<b>4</b>), ergosterol (<b>5</b>), ergosterol peroxide (<b>6</b>), chevalone C (<b>7</b>), xylaranol B (<b>8</b>), helvolic acid (<b>9</b>) and cyclo-(l-Pro-l-Leu) (<b>10</b>). The bioassay screening showed that <b>4</b> displayed cytotoxicity against KB and NCI-H187 cancer cell lines with IC<sub>50</sub> values of 3.25 and 5.95 μg mL<sup>− 1</sup>. <b>6</b> exhibited cytotoxicity against NCI-H187 with an IC<sub>50</sub> value of 5.81 μg mL<sup>− 1</sup>. <b>7</b> and <b>9</b> showed antimalarial activity with IC<sub>50</sub> values of 25.00 and 6.25 μg mL<sup>− 1</sup>, respectively. This result establishes <i>Xylaria</i> as broad spectrum bioactive compound producers.</p
Astraodoric Acids A–D: New Lanostane Triterpenes from Edible Mushroom <i>Astraeus odoratus</i> and Their Anti-<i>Mycobacterium tuberculosis</i> H<sub>37</sub>Ra and Cytotoxic Activity
Tuberculosis (TB) is one of the chronic infectious diseases
caused
by <i>Mycobacterium tuberculosis</i> that causes about 2–3
million deaths per year. Isoniazid and rifampicin are examples of
first line drugs used for TB treatment; however, they are potentially
hepatotoxic. More effective and safer drugs are urgently needed, especially
from natural products. Basidiomycete mushrooms are known as important
sources of pharmaceutically active metabolites including an anti-TB
agent. In this work, the chemical constituents of the edible mushroom <i>Astraeus odoratus</i> were isolated and investigated for antibacterial
activity against <i>M. tuberculosis</i> H<sub>37</sub>Ra.
The cytotoxic activity against cancerous cell lines was also evaluated.
Four new lanostane triterpenes, astraodoric acids A–D, and
new 5-hydroxyhypaphorine have been isolated together with four known
compounds. The structures were elucidated by NMR spectroscopic methods,
HR-ESI-MS results, and X-ray crystallographic analysis. Astraodoric
acids A and B exhibited moderate antibacterial (MICs of 50 and 25
μg/mL) and cytotoxic activities (IC<sub>50</sub> values of 34.69
and 18.57 μg/mL against KB and 19.99 and 48.35 μg/mL against
NCI-H187), respectively. The results of this study show that <i>A. odoratus</i> could be a significant natural source for safer
antitubercular and anticancer agents