The effect of the facilitated tucking position in reducing vaccination-induced pain in newborns

Background: This study was conducted to evaluate the pain perceptions of newborns during the hepatitis B (HBV) vaccinations performed in the facilitated tucking position and the classical holding position, respectively

PPAR gamma Pro 12Ala and C161T polymorphisms, but not PPAR alpha L162V, are associated with osteoporosis risk in Turkish postmenopausal women

Stimulation of peroxisome proliferator-activated receptors (PPARs) causes mesenchymal stem cells of the human bone marrow differentiate into adipocytes instead of osteoblasts leading to a decreased number of osteoblasts and a decrease in bone mineral density IBMD). Thus, PPARs may have impacts on bone metabolism. 224 postmenopausal women (171 osteoporotic and osteopenic, 53 healthy control) were included in this study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis techniques were performed to detect PPAR alpha L162V and PPAR gamma Pro12Ala/C161T polymorphisms. The distribution of PPAR gamma Pro12Ala genotype and allele frequencies was not statistically different in control and patient (osteopenic+osteoporotic) groups (p>0.05). However, in the patient group, subjects with "Pro12Pro" genotype had lower lumbar spine (L1-L4) BMD values than those with "Ala" allele (p0.05). We suggested that PPAR gamma Pro12Ala and C161T gene variants might be contributing factors in the development of osteoporosis

Efficacy and mechanisms of transcranial electrical stimulation in headache disorders

Headache is the one of the most common problems contributing to suffering worldwide and sometimes it causes disability. Some patients are unable to use the drugs for various reasons and some are resistant to the pharmacological treatment. Therefore, additional effective non-pharmacological treatments are sought.Transcranial electrical stimulation techniques have been developed as potential therapeutic options. Among these techniques, transcranial direct current stimulation (tDCS) is the only technique studied for the treatment of headache. TDCS is a neurophysiological technique with multifarious advantages encompassing its low-cost, high tolerability and acceptability, comfortable application and the opportunity to use concomitantly with other treatments. Steadily increasing interest in tDCS stems from the multidisciplinary advances in neuroscientific backgrounds of neuropsychiatric diseases. Even though exact mechanisms behind benefits of tDCS have not yet been clearly disclosed, changes in multitudinous chemical and physiological parameters have been demonstrated. The purpose of this review is to summarize what is currently known regarding the effects of tDCS on the treatment of headache focusing mostly on migraine. Herein, tDCS procedures that may be helpful for primary headache treatment were described. TDCS has shown promise for effectively treating primary headaches with no severe adverse effects. The findings indicate that the analgesic effects of tDCS can last for a long period and can occur after the time of stimulation. Additional research is required for the determination of optimized stimulation protocols in each specific headache disorder

Effect of oral vanadium supplementation on oxidative stress factors in the lung tissue of diabetic rats

Objective: Vanadium and vanadium compounds are responsible for insulin-like activity and can mimic the action of insulin through alternative signaling pathways. As the lung is a possible target organ for diabetic complications, the aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the lung tissue of diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight) to male Swiss albino rats. The rats were randomly divided into 4 groups: control; vanadyl sulfate control; STZ-diabetic untreated; STZ-diabetic treated with vanadyl sulfate. Vanadyl sulfate (100 mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, rats which were fasted overnight were sacrificed. Then, lung tissues were taken and homogenized in cold saline to make a 10% (w/v) homogenate. Antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, as well as carbonic anhydrase, myeloperoxidase and lactate dehydrogenase activities were determined in the lung tissue. Results: It was shown that vanadium supplementation decreased all enzyme activities tested which increased in the lung tissue of untreated diabetic group. Conclusion: Vanadium could be used as a preventive for diabetic complication because of its antioxidant activity

Ameliorative effect of vanadium on oxidative stress in stomach tissue of diabetic rats

Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enhancing effects. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of diabetes and on the onset of diabetic complications. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the stomach tissue of diabetic rats. Male Swiss albino rats were randomly divided into 4 groups: control; control+vanadyl sulfate; diabetic; diabetic+vanadyl sulfate. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ; 65 mg/kg body weight). Vanadyl sulfate (100 mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, stomach tissues were taken and homogenized to make a 10% (w/v) homogenate. Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), myeloperoxidase (MPO), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD) and lactate dehydrogenase (LDH) activities were determined in the stomach tissue. CAT, SOD, GR, GPx, GST, CA, G6PD and LDH activities were increased in diabetic rats when compared to normal rats. Vanadium treatment significantly reduced the elevated activities of GR, GPx, GST compared with the diabetic group whereas the decreases in CAT, SOD, CA, G6PD and LDH activities were insignificant. No significant change was seen for MPO activity between the groups. It was concluded that vanadium could be used for its ameliorative effect against oxidative stress in diabetes

Ameliorative effect of vanadium on oxidative stress in stomach tissue of diabetic rats

Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enhancing effects. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of diabetes and on the onset of diabetic complications. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the stomach tissue of diabetic rats. Male Swiss albino rats were randomly divided into 4 groups: control; control+vanadyl sulfate; diabetic; diabetic+vanadyl sulfate. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ; 65 mg/kg body weight). Vanadyl sulfate (100 mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, stomach tissues were taken and homogenized to make a 10% (w/v) homogenate. Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), myeloperoxidase (MPO), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD) and lactate dehydrogenase (LDH) activities were determined in the stomach tissue. CAT, SOD, GR, GPx, GST, CA, G6PD and LDH activities were increased in diabetic rats when compared to normal rats. Vanadium treatment significantly reduced the elevated activities of GR, GPx, GST compared with the diabetic group whereas the decreases in CAT, SOD, CA, G6PD and LDH activities were insignificant. No significant change was seen for MPO activity between the groups. It was concluded that vanadium could be used for its ameliorative effect against oxidative stress in diabetes

Ameliorative effect of vanadium on oxidative stress in stomach tissue of diabetic rats

Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enhancing effects. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of diabetes and on the onset of diabetic complications. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the stomach tissue of diabetic rats. Male Swiss albino rats were randomly divided into 4 groups: control; control+vanadyl sulfate; diabetic; diabetic+vanadyl sulfate. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ; 65 mg/kg body weight). Vanadyl sulfate (loo mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, stomach tissues were taken and homogenized to make a 10% (w/v) homogenate. Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), myeloperoxidase (MPO), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD) and lactate dehydrogenase (LDH) activities were determined in the stomach tissue. CAT, SOD, GR, GPx, GST, CA, G6PD and LDH activities were increased in diabetic rats when compared to normal rats. Vanadium treatment significantly reduced the elevated activities of GR, GPx, GST compared with the diabetic group whereas the decreases in CAT, SOD, CA, G6PD and LDH activities were insignificant. No significant change was seen for MPO activity between the groups. It was concluded that vanadium could be used for its ameliorative effect against oxidative stress in diabetes. (C) 2014 Association of Basic Medical Sciences of FB&H. All rights reserve

Combined effects of collagen type I alphal (COL1A1) Spl polymorphism and osteoporosis risk factors on bone mineral density in Turkish postmenopausal women

Identification of risk factors for osteoporosis has been essential for understanding the development of osteoporosis. The collagen type I alphal (COL1A1) gene is suggested to be implicated in reduced bone mineral density (BMD) in osteoporosis. In the present study, the investigation of the effects of Spl polymorphic variants of COL1A1 gene on BMD values, and the determination of the association between COL1A1 Spl gene variants and osteoporosis risk factors in the context of gene-environment interaction in Turkish postmenopausal women were aimed. For the detection of COL1A1 Spl polymorphism, PCR-RFLP techniques have been used. BMD for lumbar spine (L1-L4) and hip (femoral neck and total hip) was measured by DXA. This study was carried out using a sample of 254 postmenopausal women. We observed a trend decrease in BMD values in the subjects with "ss" genotype having lower BMD of lumbar spine, femoral neck and total hip than those with "SS" and "Ss" genotype, however the differences did not reach statistical significance (P > 0.05). We also found that the frequencies of the BMD under mean values at the femoral neck (57.5%) and total hip (76.2%) increased considerably in the subjects carrying "Ss/ss" genotypes in combination of having family history of osteoporosis (61.5% for femoral neck) and smoking history (90.0% for total hip). This population-based study indicates that COL1A1 Spl polymorphism may contribute to the development of osteoporosis in combination of osteoporosis risk factors in Turkish postmenopausal women. (C) 2014 Elsevier B.V. All rights reserved