Phylogenic analysis in Acacia senegal using AFLP molecular markers across the Gum Arabic Belt in Sudan

Amplified fragment length polymorphism (AFLP) DNA markers were used to characterize the genetic diversity and relationships in gum Arabic tree (Acacia senegal). Twenty eight samples of Acacia senegal collected from populations distributed throughout the Gum Arabic belt were tested in comparison with samples of Acacia mellifera and Acacia leata. Nine AFLP selective primer pair combinations generated a total of 433 amplification products with an average of 89.18% detected polymorphisms. Accessions showed the least variation was found within the A. senegal accessions in contrast with mellifera and Acacia leata that presented the highest degree of polymorphism number. According to the cluster analysis two main clusters were obtained in which A. mellifera and A. leata were placed in a separate group. There were eight subgroups of A. Senegal. Three of the eight subgroups of A. senegal were clustered according to geographical origin. The variation within population might be correlated with abiotic factors of the environment

Study of genetic diversity in Sudanese sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers

The random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Sesame indicum (L.). RAPD technique was carried out in a set of 10 sesame germplasm collected from different regions of Sudan. A total of 64 polymorphisms (6.4 polymorphic markers per primer) out of 75 reproducible products (7.5 fragments per primer) were obtained from the 10 primers used. The number of bands per primer ranged from 4 to 13, whereas the number of polymorphic bands ranged from 3 to12, corresponding to 66.6% of the amplification products. Low level of genetic similarity was observed in the collected accessions. Unique bands were observed with the 10 primers. UPGMA clustering resulted in two major groups