Host hindrance to HIV-1 replication in monocytes and macrophages

Monocytes and macrophages are targets of HIV-1 infection and play critical roles in multiple aspects of viral pathogenesis. HIV-1 can replicate in blood monocytes, although only a minor proportion of circulating monocytes harbor viral DNA. Resident macrophages in tissues can be infected and function as viral reservoirs. However, their susceptibility to infection, and their capacity to actively replicate the virus, varies greatly depending on the tissue localization and cytokine environment. The susceptibility of monocytes to HIV-1 infection in vitro depends on their differentiation status. Monocytes are refractory to infection and become permissive upon differentiation into macrophages. In addition, the capacity of monocyte-derived macrophages to sustain viral replication varies between individuals. Host determinants regulate HIV-1 replication in monocytes and macrophages, limiting several steps of the viral life-cycle, from viral entry to virus release. Some host factors responsible for HIV-1 restriction are shared with T lymphocytes, but several anti-viral mechanisms are specific to either monocytes or macrophages. Whilst a number of these mechanisms have been identified in monocytes or in monocyte-derived macrophages in vitro, some of them have also been implicated in the regulation of HIV-1 infection in vivo, in particular in the brain and the lung where macrophages are the main cell type infected by HIV-1. This review focuses on cellular factors that have been reported to interfere with HIV-1 infection in monocytes and macrophages, and examines the evidences supporting their role in vivo, highlighting unique aspects of HIV-1 restriction in these two cell types

Lawson criterion for ignition exceeded in an inertial fusion experiment

For more than half a century, researchers around the world have been engaged in attempts to achieve fusion ignition as a proof of principle of various fusion concepts. Following the Lawson criterion, an ignited plasma is one where the fusion heating power is high enough to overcome all the physical processes that cool the fusion plasma, creating a positive thermodynamic feedback loop with rapidly increasing temperature. In inertially confined fusion, ignition is a state where the fusion plasma can begin "burn propagation" into surrounding cold fuel, enabling the possibility of high energy gain. While "scientific breakeven" (i.e., unity target gain) has not yet been achieved (here target gain is 0.72, 1.37 MJ of fusion for 1.92 MJ of laser energy), this Letter reports the first controlled fusion experiment, using laser indirect drive, on the National Ignition Facility to produce capsule gain (here 5.8) and reach ignition by nine different formulations of the Lawson criterion

A study of ambient upstream material properties using perpendicular laser driven radiative blast waves in atomic cluster gases

We report on the characterisation of the upstream medium ahead of a radiative cylindrical blast wave launched in an argon cluster gas with a 1 J, 1 ps, 1054 nm Nd:Glass laser system. By launching two perpendicular blast waves and introducing a time delay between the heating beams it is possible to determine the extent of the cluster medium by observing the high energy absorption region associated with clusters, as apposed to the low energy deposition in monatomic gas. It was found that argon ions launched from the initial laser driven cluster ionisation created a ballistic ion wave which sweeps out ahead of the hydrodynamic blast wave at an initial velocity of 1000 kms -1. This ballistic wave disassembles the clusters ahead of the blast wave into a neutral gas medium before the arrival of a radiative precursor. This observation gives us confidence that the dynamics of a radiative blast wave in cluster based experiments is determined primarily by the properties of an upstream atomic gas, and is not significantly influenced by cluster affects on energy transport or other material properties. © 2011 Elsevier B.V

Spectral enhancement in optical parametric amplifiers in the saturated regime

We present numerical and experimental studies on a front-end laser system for seeding a sub-picosecond multi-terawatt/petawatt light source based on two highly saturated optical parametric amplifiers. Numerical results showing energy gain, spectral gain, and stability as saturation is approached are presented. An experimental realisation of the system has been constructed, and its performance has been found to be in good agreement with the numerical simulations. The total gain is in excess of 105 over a few picosecond gain window, and the pulse energy is ∼100 μJ with 4 % root mean square stability. © 2013 Springer-Verlag Berlin Heidelberg

The phase-2 particle x-ray temporal diagnostic for simultaneous measurement of multiple x-ray and nuclear emission histories from OMEGA implosions (invited)

Electron-temperature (T e) measurements in implosions provide valuable diagnostic information, as T e is negligibly affected by residual flows and other non-thermal effects unlike ion-temperature inferred from a fusion product spectrum. In OMEGA cryogenic implosions, measurement of T e(t) can be used to investigate effects related to time-resolved hot-spot energy balance. The newly implemented phase-2 Particle X-ray Temporal Diagnostic (PXTD) utilizes four fast-rise (∼15 ps) scintillator-channels with distinct x-ray filtering. Titanium and stepped aluminum filtering were chosen to maximize detector sensitivity in the 10–20 keV range, as it has been shown that these x rays have similar density and temperature weighting to the emitted deuterium–tritium fusion neutrons (DTn) from OMEGA Cryo-DT implosions. High quality data have been collected from warm implosions at OMEGA. These data have been used to infer spatially integrated T e(t) with &lt;10% uncertainty at peak emission. Nuclear and x-ray emission histories are measured with 10 ps relative timing uncertainty for x rays and DTn and 12 ps for x rays and deuterium-[Formula: see text] protons (D3Hep). A future upgrade to the system will enable spatially integrated T e(t) with 40 ps time-resolution from cryogenic DT implosions. </jats:p