Untersuchungen zum Einfluss einer Air-Liquid-Interphase auf die Differenzierung von Alveolarepithelzellen Typ II

Die Dedifferenzierung der Alveolarepithelzellen Typ II stellt in vitro ein großes Problem dar. Zellen, die länger als 48 Stunden in vitro kultiviert werden tendieren zu einer Dedifferenzierung. Die vorliegende Arbeit stellt ein Air-Liquid-Interphasesystem zur Kultivierung von TypII-Zellen vor. Hierdurch ist es uns gelungen TypII-Zellen über einen Zeitraum von bis zu 14 Tagen in vitro auf einem hohen Grad der Differenzierung zu konservieren. Zellen die nicht so kultiviert wurden zeigten früh (48 Stunden) eine Dedifferenzierung. Untersucht wurden die Morphologie, metabolische Eigenschaften (Surfactanthaushalt), inflammatorische Faktoren und der Einfluß von Hypoxie.Type II alveolar epithelial cells still tend to lose their high differentiated properties when cultivating in vitro. We present a new cultivation modell in which type II cells show a high state of differentiation even when cultivating over 14 days in vitro. We studied the tendency of loosing high differentiation factors in this air-liquid-interhasemodell analyzing inflammatory factors, metabolic factors (surfactant system) and morphologic aspects of the cells. The used modell is a good in vitro modell for conservation type II cell differentiation

Un recente contributo su Marziale e l’epigramma longum

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Understanding the link between audience engagement metrics and the perceived quality of online news using machine learning

This article aims to explain the perceived quality of online news articles. Discovering which elements of a news story influence readers' perceptions could drive online popularity, which is the paramount factor of digital news readership. This work explores an approach to use tree-based machine learning algorithms to address this problem based on selected characteristics, which measure engagement, drawn from prior research mostly developed by communication scientists. A proposed extended model is used to examine the association between the engagement features and perceived quality concerning all the articles depending mainly on their genre. To demonstrate the capacity of using predictive analytics to facilitate journalistic news writing the proposed methodology is applied on a novel data set with 200K articles in total constructed from a blog site. The results of phase A, indicate interesting correlations between the features and the perceived quality of the articles. In stage B, the paper seeks to extract a set of rules that can be used as guidelines for authors in the writing of their next articles, indicating the probability of popularity that their articles may gain if these rules are taken into consideration

Functional recovery after implantation of artificial nerve grafts in the rat- a systematic review

<p>Abstract</p> <p>Purpose</p> <p>The aim of this study was to compare functional data of different nerve-gap bridging materials evaluated in rat experiments by means of a systematic review.</p> <p>Materials and methods</p> <p>A systematic review was conducted, searching MEDLINE, HTS and CENTRAL to identify all trials evaluating functional recovery of artificial nerve conduits in the rat model.</p> <p>Results</p> <p>There was a trend towards a favourable outcome of conduits coated with Schwann-cells compared to the plain synthetics. Histomorphometry, electrophysiology and muscle-weight correlated poorly with functional outcome.</p> <p>Conclusion</p> <p>Schwann-cell coated conduits showed promising results concerning functional recovery. Further standardization in outcome reporting is encouraged.</p

Length-weight relationships of freshwater fishes in Greece

Length-weight relationships were calculated for nine fish species from Lake Volvi (Macedonia, Hellas), caught with gillnets of five different mesh sizes between October 1995 and October 1996. In addition, length-weight relationships for 24 Greek freshwater fish species and one hybrid were also obtained from the literature. The values of the exponent of the length-weight relationships for all fish species examined ranged between 2.14 and 3.70 (mean = 3.12; SE=0.0321), and the median value was 3.19

Does Pulsed Magnetic Field Therapy Influence Nerve Regeneration in the Median Nerve Model of the Rat?

The aim of this study was to evaluate the impact of pulsed magnetic field therapy on peripheral nerve regeneration after median nerve injury and primary coaptation in the rat. Both median nerves were surgically exposed and denervated in 24 female Wistar rats. A microsurgical coaptation was performed on the right side, whereas on the left side a spontaneous healing was prevented. The study group underwent a daily pulsed magnetic field therapy; the other group served as a control group. The grasping force was recorded 2 weeks after the surgical intervention for a period of 12 weeks. The right median nerve was excised and histologically examined. The histomorphometric data and the functional assessments were analyzed by t-test statistics and one-way ANOVA. One-way ANOVA indicated a statistically significant influence of group affiliation and grasping force (P=0.0078). Grasping strength was higher on a significant level in the experimental group compared to the control group permanently from the 9th week to the end of the study. T-test statistics revealed a significantly higher weight of the flexor digitorum sublimis muscle (P=0.0385) in the experimental group. The histological evaluation did not reveal any statistically significant differences concerning the histomorphometric parameters. Our results suggest that the pulsed magnetic field therapy has a positive influence on the functional aspects of neural regeneration. More studies are needed to precisely evaluate and optimize the intensity and duration of the application