424 research outputs found

    Foreword to C. Th. Dimaras Annual Lecture, 2019

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    Foreword to C. Th. Dimaras Annual Lecture, 201

    Nietzsche and Heraclitus: Notes on Stars without an Atmosphere

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    I awake estranged from everyone. Words have lost their meaning; they sound indifferent and homonymous. The word No appears to mean Yes, or rather: Yes and No are malleable, ephemeral, and transparent. A decades-old or perhaps centuries-old movement of miry clay has resulted in a miscarriage of words. Iinquire whether anyone still holds the resources needed for a direct, sincere affirmation of lifeā€”a Yes that is definitively and essentially affirmativeā€”or a No that is definitively and essentially negativeā€”words bursting forth splendour like a crystal. I am told that formulations of this sort are incomprehensible; they are too metaphorical and, in the end, nonsensical, at least according to the elenctic criteria of analytical philosophy and common sense..

    TMF is a golgin that binds Rab6 and influences Golgi morphology

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    BACKGROUND: Golgins are coiled-coil proteins associated with the Golgi apparatus, that are believed to be involved in the tethering of vesicles and the stacking of cisternae, as well as other functions such as cytoskeletal association. Many are peripheral membrane proteins recruited by GTPases. Several have been described in animal cells, and some in yeast, but the relationships between golgins from different species can be hard to define because although they share structural features, their sequences are not well conserved. RESULTS: We show here that the yeast protein Sgm1, previously shown to be recruited to the Golgi by the GTPase Ypt6, binds to Ypt6:GTP via a conserved 100-residue coiled-coil motif that can be identified in a wide range of eukaryotes. The mammalian equivalent of Sgm1 is TMF/ARA160, a protein previously identified in various screens as a putative transcription or chromatin remodelling factor. We show that it is a Golgi protein, and that it binds to the three known isoforms of the Ypt6 homologue Rab6. Depletion of the protein by RNA interference in rat NRK cells results in a modest dispersal of Golgi membranes around the cell, suggesting a role for TMF in the movement or adherence of Golgi stacks. CONCLUSION: We have identified TMF as an evolutionarily conserved golgin that binds Rab6 and contributes to Golgi organisation in animal cells

    Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2

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    Peer reviewedPublisher PD

    Spatial distribution of lipid droplets during starvation: Implications for lipophagy.

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    Survival during starvation depends largely on metabolic energy, which is stored in the form of neutral lipids in specialized organelles known as lipid droplets. The precursors for the synthesis of neutral lipids are also used for membrane biogenesis, which is required for cell growth and proliferation. Therefore cells must possess mechanisms to preferentially channel lipid precursors toward either membrane synthesis or lipid droplet storage, in response to nutrient status. How this partitioning is spatially regulated within the endoplasmic reticulum (ER) where lipid droplets co-localize, remains poorly understood. We have recently shown that at the onset of starvation lipid droplets concentrate at a perinuclear ER subdomain flanking the nucleus-vacuole junction (NVJ) and that this is crucial for maintaining proper nuclear shape and ER membrane organization. Here we show that disruption of the NVJ does not block the translocation and internalization of lipid droplets into the vacuole for their degradation, which takes place at later stages of starvation. We propose that alternative pathways of lipid droplet translocation from the ER to the vacuole may exist to enable stationary phase-induced lipophagy.This work was supported by the Medical Research Council (G0701446) and the Wellcome Trust (108042/Z/15/Z)
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