DMAP-catalysed synthesis, antibacterial activity evaluation, cytotoxicity and docking studies of some heterocyclic molecules bearing sulfonamide moiety

DMAP has been shown to be a highly efficient nucleophilic catalyst when compared to triethylamine and pyridine using acetonitrile as solvent for the synthesis of a series of novel N- heterocyclic sulfonamide derivatives. The influence of the reaction parameters, like choice of solvent, catalyst, amount of catalyst and reaction time on product yield has been studied. Antibacterial screening involving a range of sulfonamide analogues as new peptide deformylase (PDF) inhibitors have been focused. The molecules show significant antibacterial activity (MIC value 6.2 − 3.1 µg/mL) against B. subtilis, S. pyrogenes, P. vulgaris and P. mirabilis. Potential in silico docking studies have been in conjugation with in vitro antibacterial results. Molecular docking of all compounds with PDF enzyme (PDB code: 1G2A) explain how certain moieties play significant roles in increasing the binding interactions and stabilizing the protein-ligand complexes. The compounds also have confirmed low extent of cytotoxicity when tested on HEL and HeLa cell lines

Therapeutic Uses of Curcumin Spice

Dr. Singh discusses the therapeutics uses of curcumin (turmeric). It has been found to be a potent antioxidant, anti-inflammatory, antibacterial, antiviral and anti-cancer agent

Docking studies on novel bisphenylbenzimidazoles (BPBIs) as non-nucleoside inhibitors of HIV-1 reverse transcriptase

714-723The low toxicity of non-nucleoside reverse transcriptase inhibitors (NNRTIs) in comparison to nucleoside reverse transcriptase inhibitors (NRTIs) has triggered the idea of preparing better and safer second generation NNRTIs. In this direction, a series of N-1 and C-2 Bisphenylbenzimidazoles (BPBIs)have been designed in silicoas possible NNRTIs. On the basis of Lipinski’s rule of five, compounds having drug like character have been docked into the active site of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) enzyme (PDB: ID 1HNV) using the software Discovery Studio   2.5. Analysis of the docking results reveal that all molecules form hydrogen bonds with amino acids Lys101, Lys103, Tyr181, Tyr318 and exhibit π-stacking interactions with Tyr181, Tyr188, Phe227 and Trp229 present in the non- nucleoside inhibitor binding pocket (NNIBP). The designed ligands have adopted butterfly conformation inside the NNIBP and form more stable complexes (total interaction energy found in the range of (-) 45.05 - (-) 62.54 kcal/mol) with HIV-1 RT in comparison to TIBO and nevirapine (NVP) (-) 47.70 and (-) 45.99 Kcal/mol, respectively, and thus, lower EC50 values are predicted for BPBIs. The results provide insight into predictive and diagnostic aspects for better activity of this class of HIV-1 RT inhibitors

Synthesis and antibacterial activity of benzamides and sulfonamide derived from 2-amino-5-bromo/nitropyridine against bacterial strains isolated from clinical patients

931-936A series of 5-(bromo/nitropyridin-2-yl)benzamide derivatives, viz. N-(5-bromopyridin-2-yl)benzamide 4a, 4-methyl-N-(5-bromopyridin-2-yl)benzamide 4b, 4-chloro-N-(5-bromopyridin-2-yl)benzamide 4c, 4-nitro-N-(5-bromopyridin-2-yl)benzamide 4d, N-(5-nitropyridin-2-yl)benzamide 7a, 4-methyl-N-(5-nitropyridin-2-yl)benzamide 7b, 4-chloro-N-(5-nitropyridin-2-yl)benzamide 7c, 4-nitro-N-(5-nitropyridin-2-yl)benzamide 7c and a sulfo­namide derivative, N-benzoyl-N-(5-bromopyridin-2-yl)trifluoro­methane sulfonamide 5 have been synthesized  as novel  anti­bacterial agents. All compounds have shown promising activity (MIC 0.22–1.49 M) against Gram-positive and Gram-negative bacterial strains using microdilution broth susceptibility test method. The sulfonamide 5 showed much better results than other compounds and its MIC values predicted it to be a lead compound as an antibacterial agent

Facile synthesis of oligonucleotides on solid support using pyridine derivatives for amino and phosphate protection

1696-1700The picolinoyl group has been used for amino protection in the case of all the three deoxynucleosides-dC, dA and dG, and good yields of N-protected nucleosides are obtained. This pyridine derivative along with another pyridine derivative, viz. (α-pyridyl) methyl, an autocatalytic phosphate protecting group, has been used successfully in the synthesis of two oligonucleotides, d(TACGTTTTGCT) and d(ACCGATATCGT) following solid phase methodology. The good yields of these 11-mers (48 and 45%, respectively) are attributed to the greater solubilising effect generated due to the combination of these two groups. The structures of these oligomers have been confirmed by enzymatic hydrolysis with snake venom phosphodiesterase followed by alkaline phosphatase