Honeybees Learn Odour Mixtures via a Selection of Key Odorants

BACKGROUND The honeybee has to detect, process and learn numerous complex odours from her natural environment on a daily basis. Most of these odours are floral scents, which are mixtures of dozens of different odorants. To date, it is still unclear how the bee brain unravels the complex information contained in scent mixtures. METHODOLOGY/PRINCIPAL FINDINGS This study investigates learning of complex odour mixtures in honeybees using a simple olfactory conditioning procedure, the Proboscis-Extension-Reflex (PER) paradigm. Restrained honeybees were trained to three scent mixtures composed of 14 floral odorants each, and then tested with the individual odorants of each mixture. Bees did not respond to all odorants of a mixture equally: They responded well to a selection of key odorants, which were unique for each of the three scent mixtures. Bees showed less or very little response to the other odorants of the mixtures. The bees' response to mixtures composed of only the key odorants was as good as to the original mixtures of 14 odorants. A mixture composed of the other, non-key-odorants elicited a significantly lower response. Neither an odorant's volatility or molecular structure, nor learning efficiencies for individual odorants affected whether an odorant became a key odorant for a particular mixture. Odorant concentration had a positive effect, with odorants at high concentration likely to become key odorants. CONCLUSIONS/SIGNIFICANCE Our study suggests that the brain processes complex scent mixtures by predominantly learning information from selected key odorants. Our observations on key odorant learning lend significant support to previous work on olfactory learning and mixture processing in honeybees.This work was supported by a grant from the Commonwealth Scientific and Industrial Research Organisation Food Futures Flagship Collaborative Research Fund (CBR3_45865_9 W2003, http://www.csiro.au/org/FoodFuturesFlagship.html). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Chukotsk or Chukchi: Some Thoughts on the Transposition of Soviet Geographical Names

... To some cartographers and gazetteer writers the transposition of Russian geographical names into English may seem a subject well suited for an arbitrary rule of thumb; but is it? ... The reason for the current trend towards indiscriminate transliteration of Soviet geographical names is not far to seek. It is the simplest way to avoid the problems of adaptation, and at the same time enables all those who may now have a need to know the Russian version of the names to avoid learning the 32 letters of the Cyrillic alphabet - at the price of a minor distortion and some uncertainty in spelling and pronunciation. By the same token it is also the crudest manner of transposition. It literally deprives the English language of a galaxy of geographical names for a vast and increasingly important part of the earth. It is a way of dodging an issue instead of facing it. ..

Insect Immunity Varies Idiosyncratically During Overwintering.

Overwintering insects face multiple stressors, including pathogen and parasite pressures that shift with seasons. However, we know little of how the insect immune system fluctuates with season, particularly in the overwintering period. To understand how immune activity changes across autumn, winter, and spring, we tracked immune activity of three temperate insects that overwinter as larvae: a weevil (Curculio sp., Coleoptera), gallfly (Eurosta solidaginis, Diptera), and larvae of the lepidopteran Pyrrharctia isabella. We measured baseline circulating hemocyte numbers, phenoloxidase activity, and humoral antimicrobial activity, as well as survival of fungal infection and melanization response at 12°C and 25°C to capture any potential plasticity in thermal performance. In Curculio sp. and E. solidaginis, hemocyte concentrations remained unchanged across seasons and antimicrobial activity against Gram-positive bacteria was lowest in autumn; however, Curculio sp. were less likely to survive fungal infection in autumn, whereas E. solidaginis were less likely to survive infection during the winter. Furthermore, hemocyte concentrations and antimicrobial activity decreased in P. isabella overwintering beneath snow cover. Overall, seasonal changes in activity were largely species dependent, thus it may be difficult to create generalizable predictions about the effects of a changing climate on seasonal immune activity in insects. However, we suggest that the relationship between the response to multiple stressors (e.g., cold and pathogens) drives changes in immune activity, and that understanding the physiology underlying these relationships will inform our predictions of the effects of environmental change on insect overwintering success

Which Extratropical Cyclones Contribute Most to the Transport of Moisture in the Southern Hemisphere?

Predicted changes in Southern Hemisphere (SH) precipitation and Antarctic ice mass correspond to variations in the meridional moisture flux (MMF). Thirty-five years of ERA-Interim reanalysis data are combined with an extratropical cyclone (ETC) identification and tracking algorithm to investigate factors controlling SH MMF variability in the midlatitudes and near Antarctica. ETC characteristics which exert the strongest control on ETC MMF are determined thus identifying which ETCs contribute most to SH moisture transport. ETC poleward propagation speed exerts the strongest control on the ETC MMF across the Antarctic coastline. In SH winter, ETCs with the largest poleward propagation speeds transport 2.5 times more moisture than an average ETC. In the midlatitudes, ETC genesis latitude and poleward propagation speed have a similar influence on ETC MMF. Surprisingly, ETC maximum vorticity has little control on ETC MMF. Cyclone compositing is used to determine the reasons for these statistical relationships. ETCs generally exhibit a dipole of poleward and equatorward MMF downstream and upstream of the cyclone center, respectively. However, ETCs with the largest poleward propagation speeds resemble open frontal waves with strong poleward moisture transport downstream of the cyclone center only and thus result in the largest MMF. These results suggest that inhomogeneous trends and predicted changes in precipitation over Antarctica may be due to changes in cyclone track orientation, associated with changes to the large-scale background flow, in addition to changes in cyclone number or intensity.Peer reviewe

Thermal variability and plasticity drive the outcome of a host-pathogen interaction

Variable, changing, climates may affect each participant in a biotic interaction differently. We explored the effects of temperature and plasticity on the outcome of a host-pathogen interaction to try to predict the outcomes of infection under fluctuating temperatures. We infected Gryllus veletis crickets with the entomopathogenic fungus Metarhizium brunneum under constant (6 °C, 12 °C, 18 °C or 25 °C) or fluctuating temperatures (6 °C to 18 °C or 6 °C to 25 °C). We also acclimated crickets and fungi to constant or fluctuating conditions. Crickets acclimated to fluctuating conditions survived best under constant conditions if paired with warm-acclimated fungus. Overall, matches and mismatches in thermal performance, driven by acclimation, determined host survival. Mismatched performance also determined differences in survival under different fluctuating thermal regimes: crickets survived best when fluctuating temperatures favoured their performance (6 °C to 25 °C), compared to fluctuations that favoured fungus performance (6 °C to 18 °C). Thus, we could predict the outcome of infection under fluctuating temperatures by averaging relative host-pathogen performance under constant temperatures, suggesting that it may be possible to predict responses to fluctuating temperatures for at least some biotic interactions

Bose-Einstein Condensation on a Permanent-Magnet Atom Chip

We have produced a Bose-Einstein condensate on a permanent-magnet atom chip based on periodically magnetized videotape. We observe the expansion and dynamics of the condensate in one of the microscopic waveguides close to the surface. The lifetime for atoms to remain trapped near this dielectric material is significantly longer than above a metal surface of the same thickness. These results illustrate the suitability of microscopic permanent-magnet structures for quantum-coherent preparation and manipulation of cold atoms.Comment: 4 pages, 6 figures, Published in Phys. Rev. A, Rapid Com

Bose-Einstein Condensation on a Permanent-Magnet Atom Chip

We have produced a Bose-Einstein condensate on a permanent-magnet atom chip based on periodically magnetized videotape. We observe the expansion and dynamics of the condensate in one of the microscopic waveguides close to the surface. The lifetime for atoms to remain trapped near this dielectric material is significantly longer than above a metal surface of the same thickness. These results illustrate the suitability of microscopic permanent-magnet structures for quantum-coherent preparation and manipulation of cold atoms.Comment: 4 pages, 6 figures, Published in Phys. Rev. A, Rapid Com

Adversary Juvenile Delinquency Proceedings: Impeachment of Juvenile Defendants by the Use of Previous Adjudications of Delinquency

IN ADULT CRIMINAL PROCEEDINGS, any defendant who wishes to testify\u27 faces certain risks when he steps into the witness box. The risks such a defendant engenders certainly include the possibility of having his prior criminal convictions brought up by the prosecution, for purposes of impeaching his testimony. In essence, the defendant who takes the stand, like any other witness, places his reputation for truth and veracity into issue. The theory behind this general rule emanates from the belief that the defendant\u27s testimony can be no more credible than the defendant himself. Therefore, the prosecution is given the right, under certain limitations, to attack the credibility of the defendant and his testimony by inquiring into his past criminal conduct