Renal and systemic parameters 7 and 45 days after renal ablation.

<p>Mean ±1 SE; BW: body weight, grams; TCP: tail-cuff pressure, mmHg; U_albV: daily urinary albumin excretion rate, mg/24 h; Ht, arterial hematocrit, %; S_Cr: serum creatinine concentration, mg/dL. ^ap<0.05 vs. respective S; ^bp<0.05 vs. respective untreated; ^cp<0.05 vs. respective value on Day 7.</p

RT-PCR analysis of the expression of VEGF (A) and of its receptors, VEGFR1 (B), VEGFR2 (C), and VEGFR3 (D).

<p>^ap<0.05 vs. respective S; ^bp<0.05 vs. respective untreated; ^cp<0.05 vs. respective value on Day 7.</p

Evaluation of tubulointerstitial changes.

<p>A) Fractional cortical interstitial area; B) Cortical interstitial infiltration by macrophages; C) Tubular cells in proliferation (PCNA⁺); D) Interstitial cells in proliferation (PCNA⁺);^ap<0.05 vs. respective S; ^bp<0.05 vs. respective untreated; ^cp<0.05 vs. respective value on Day 7.</p

Vascular endothelial evaluation.

<p>A) % glomerular area occupied by endothelium; B) Number of peritubular capillary profiles. ^ap<0.05 vs. respective S; ^bp<0.05 vs. respective untreated; ^cp<0.05 vs. respective value on Day 7.</p

Histologic and histomorphometric analysis of glomerular injury (Masson Trichrome).

<p>A) and B) normal glomeruli found in Group S+V and S+Su, respectively; C) and D) representative glomerular sclerotic lesions found in Group Nx+V and Nx+ Su. E) glomerular intracapillary microthrombi observed in Group Nx+Su, some of which appear partially organized. F) PTAH staining showing the presence of fibrin (in dark purple) in glomerular capillary lumina of Nx+Su rats. G) distribution of glomerular lesions among “microthrombi” (red) and “sclerosis” (blue). Normal glomeruli are represented in gray. ^ap<0.05 vs. respective S; ^bp<0.05 vs. respective untreated; ^cp<0.05 vs. respective value on Day 7.</p

A) Glomerular volume; B) glomerular cell proliferation assessed by the number of PCNA⁺ cells; C) Percentage of glomerular area staining positively for ZO-1; D) Number of podocytes per glomerulus.

<p>^ap<0.05 vs. respective S; ^bp<0.05 vs. respective untreated; ^cp<0.05 vs. respective value on Day 7.</p