15 research outputs found
Chromatographic analysis of immobilized cefotaxime
The aim of the present work was to widen the application of an in-house
developed fast, flexible and sensitive high performance liquid chromatography
(HPLC) method to the assessment of cefotaxime sodium from aqueous samples.
The method was applied to establish the release profile of cefotaxime sodium
immobilised in MCM-41 nanoparticles using pH controlled release in aqueous
medium. The analytical method proved to be sensible, repeatable (RSD < 1.5 %)
and reproducible (RSD < 1 %) in the concentration range studied (0.01-10
μg•mL-1), limit of detection and limit of quantification were 0.036 and 0.12
μg•mL-1 respectively, suitable for the analysis of release of a single active
ingredient, having a short analysis time (10 minutes)
Biosensors Applications on Assessment of Reactive Oxygen Species and Antioxidants
Computer networking & communication
Fourier Transform Infrared Spectroscopy - Useful Analytical Tool for Non-Destructive Analysis
Email: consumer/user guide
Membrane processes application on the Symphytum officinale and Geranium robertianum extracts concentration to obtain high antioxidative activity compounds
The paper reports the successful application of membranes processes to obtain the good quality extracts with compounds of high antioxidative activity and therapeutic value. This study provided investigations on the phenolic compounds from two plant species used in Romanian ethno-medicine and evaluated the antioxidant and cytotoxic activities. Three extracts prepared from Geranium robertianum and Symphytum officinale were concentrated by microfiltration and ultrafiltration. The levels of phenolic compounds and flavonoids were determined by UV-Vis spectroscopy and HPLC. The freeradical scavenging activity of concentrated extracts was determined by DPPH method. The preliminary tests of cytotoxic activity for the concentrated extracts have been carried out on human epidermoid laryngeal carcinoma cell line (Hep-2p) and normal monkey kidneys cells (RM). The results showed that all concentrated extracts had a very low cytotoxicity against healthy cells, but a significant cytotoxic effect on Hep-2p tumor cells. The concentrated extracts have a high antioxidant activity (% DPPH inhibition > 80%)
Chapter Fourier Transform Infrared Spectroscopy - Useful Analytical Tool for Non-Destructive Analysis
Email: consumer/user guide
5 Biosensors Applications on Assessment of Reactive Oxygen Species and Antioxidants
Computer networking & communication