1 research outputs found
Cell Adhesion on Surface-Functionalized Magnesium
The biocompatibility of commercially
pure magnesium-based (cp Mg) biodegradable implants is compromised
of strong hydrogen evolution and surface alkalization due to high
initial corrosion rates of cp Mg in the physiological environment.
To mitigate this problem, the addition of corrosion-retarding alloying
elements or coating of implant surfaces has been suggested. In the
following work, we explored the effect of organic coatings on long-term
cell growth. cp Mg was coated with aminopropyltriehtoxysilane + vitamin
C (AV), carbonyldiimidazole (CDI), or stearic acid (SA). All three
coatings have been previously suggested to reduce initial corrosion
and to enhance protein adsorption and hence cell adhesion on magnesium
surfaces. Endothelial cells (DH1+/+) and osteosarcoma cells (MG63)
were cultured on coated samples for up to 20 days. To quantify Mg
corrosion, electrochemical impedance spectroscopy (EIS) was measured
after 1, 3, and 5 days of cell culture. We also investigated the speed
of initial cell spreading after seeding using fluorescently labeled
fibroblasts (NIH/3T3). Hydrogen evolution after contact with cell
culture medium was markedly decreased on AV- and SA-coated Mg compared
to uncoated Mg. These coatings also showed improved cell adhesion
and spreading after 24 h of culture comparable to tissue-treated plastic
surfaces. On AV-coated cp Mg, a confluent layer of endothelial cells
formed after 5 days and remained intact for up to 20 days. Together,
these data demonstrate that surface coating with AV is a viable strategy
for improving long-term biocompatibility of cp Mg-based implants.
EIS measurements confirmed that the presence of a confluent cell layer
increased the corrosion resistance