Fetal lung gene array data for <i>AGER</i> expression across Pseudoglandular and Canalicular stages.

<p>Fetal lung gene array data for <i>AGER</i> expression across Pseudoglandular and Canalicular stages.</p

<i>AGER</i> isoform expression in three HBEC donors using RNA Seq.

<p>Structure and abundance of known <i>AGER</i> isoforms in three human bronchial epithelial cell donors illustrating heterogeneity in expression levels. Percentage abundances (% FPKM) were calculated for each donor. Transcripts for full length and soluble <i>AGER</i> were identified at similar low abundancies. FPKM; fragments per kilobase of transcript per million mapped reads.</p

<i>AGER</i> mRNA expression levels in total lung and airway cells.

<p>Q-PCR analysis identified <i>AGER</i> mRNA was highly expressed in total lung and at lower levels in human airway smooth muscle cells (HASM) cells, human bronchial epithelial cells (HBEC) and the BEAS-2BR1 bronchial epithelial cell line, (n = 3).</p

Immunohistochemical analysis of RAGE expression in healthy and COPD lung.

<p>In healthy lung tissue, RAGE was found to be localised to the cytoplasm and membrane. RAGE expression was high in the pneumocytes of alveolar regions (a). The bronchial epithelium showed variable weak to moderate staining (e). In lung tissue of individuals with COPD, RAGE was very strongly immunopositive in the membrane and cytoplasm of the pneumocytes in the alveolar regions (b). The bronchial epithelium from individuals with COPD was weak or immunonegative for the RAGE protein (f). All isotype controls were negative (c, d, g and h). Representative images of one healthy and one COPD lung shown. x10 magnification.</p

Gene expression array data of 38 fetal lungs for the <i>AGER</i> probes.

<p>Expression intensities for the <i>AGER</i> probes 210081_at and 217046_s_at were plotted against the gestational age of each fetal lung sample and showed an increase in expression with fetal lung age. RMA; Robust Multi-array Average. Affymetrix U133 Plus 2 expression array probe 210081_at probes for exon 8 of AGER mRNA whilst 217046_s_at probes AGER exons 7–11’.</p

Baseline characteristics of UK smoker population used for genetic association studies.

<p>Baseline characteristics of UK smoker population used for genetic association studies.</p

The genotype effect of rs2070600.

<p>Serum sRAGE levels are lower in individuals with the C:T genotype at rs2070600 (p<0.0001, Mann-Whitney) (6i). Cells expressing RAGE-Ser82 (T allele) stimulated with HMGB1 for 48 hours had lower levels of sRAGE than RAGE-Gly82 (C allele) carrying cells (n = 4, p = 0.036), (6ii).</p