Validated age and growth estimates for Carcharhinus obscurus in the northwestern Atlantic Ocean, with pre- and post management growth comparisons

This paper is not subject to U.S. copyright. The definitive version was published in Environmental Biology of Fishes 97 (2014): 881-896, doi:10.1007/s10641-013-0189-4.Age and growth estimates for the dusky shark, Carcharhinus obscurus, were derived from vertebral centra collected in the northwestern Atlantic Ocean. Sample collection spanned the years prior to and following the implementation of management measures (1963–2010). Growth was compared pre- and post- population depletion and pre- and post- management to investigate the possibility of density-mediated shifts in age and growth parameters over time. There was no evidence of difference between periods for either sex. Additionally, bomb radiocarbon dating was used to determine the periodicity of band pair formation. Results support the traditional interpretation of annual band pairs up to approximately 11 years of age. After this time, vertebral counts considerably underestimate true age. Maximum validated ages were estimated to be between 38 and 42 years of age (an increase of 15 to 19 years over the band count estimates), confirming longevity to at least 42 years of age. Growth curves estimated using only validated data were compared to those generated using band pair counts. Logistic growth parameters derived from validated vertebral length-at-age data were L ∞  = 261.5 cm FL, L o  = 85.5 cm, t o  = 4.89 year and g = 0.15 year−1 for the sexes combined. Revised estimates of age at maturity were 17.4 years for males and 17.6 years for females

Global Phylogeography of the Dusky Shark Carcharhinus obscurus: Implications for Fisheries Management and Monitoring the Shark Fin Trade

Genetic stock structure information is needed to delineate management units and monitor trade in sharks, many of which are heavily exploited and declining. The dusky shark Carcharhinus obscurus is a large apex predator that is sought after for its fins and is considered highly susceptible to overexploitation. The International Union for the Conservation of Nature (IUCN) classifies this species as ‘Vulnerable’ globally and ‘Endangered’ in the northwest Atlantic. We make the first assessment of global stock structure of C. obscurus by analyzing part of the mitochondrial control region (mtCR) in 255 individuals sampled from 8 geographically dispersed locations. We found 25 mtCR haplotypes and rejected a null hypothesis of panmixia (analysis of molecular variance, ΦST = 0.55, p \u3c 0.000001), detecting significant differentiation between 3 management units: US Atlantic (USATL), South Africa (SAF), and Australia (AUS). We also found preliminary evidence of population structure between the USATL and southwest Atlantic (Brazil). There were no shared haplotypes between the western Atlantic and Indo-Pacific. These analyses suggest that replenishment of the collapsed USATL management unit via immigration of females from elsewhere is unlikely. Mixed stock analysis (MSA) simulations show that reconstruction of the relative contributions of USATL, SAF, and AUS management units to the Asian fin trade is possible using these mtCR sequences. We suggest avenues for obtaining samples to conduct MSA of the shark fin trade, which could enhance management of dusky sharks and other species that are exploited for their fins

Phylogenetics and phylogeography of the bull shark, Carcharhinus leucas (Valenciennes, 1839)

The bull shark, Carcharhinus leucas, is an important euryhaline predator that is exploited throughout its range for meat and fins. This species is taxonomically paired with the pigeye shark, C. amboinensis. Validity of this group has not been directly tested using molecular markers. Genetic variation and phylogeny was examined with concatenated datasets of mitochondrial genes (cytb, cox1 and 12stRNA16s) for nineteen species, from three families within the order Carcharhiniformes. Whilst there were indications of species pairings within the genus, there was no evidence to suggest that the bull and pigeye sharks should be considered a species group. Phylogenetic analysis failed to resolve Carcharhinus, but confirmed Negaprion as a sister taxon and placed Prionace glauca within Carcharhinus. It remains unclear if the family Sphyrnidae, arose from a Rhizoprionodon or Scoliodon ancestor and future revision of this complex group of sharks is required. Like other large carcharhinid species, C. leucas exhibits a low reproductive rate and long generation times. It is susceptible to localised depletions and such declines have been documented. The global stock structure was assessed by analysing the mitochondrial control region in 245 individuals sampled from eight populations in three ocean basins. The bull shark exhibits relatively high haplotype diversity (0.896 ± 0.010) when compared to other globally-distributed sharks and the nucleotide diversity was similar to others from the genus (0.00465 ± 0.00014). There was significant stock structure found among populations (ΦST=0.736, p<0.00001) and among ocean basins (ΦCT=0.527, p=0.00653). Geneflow between the US Atlantic coastline and Gulf of Mexico was sufficient to consider the area to be a single panmictic population. Coalescent analyses suggest an Indian Ocean origin with population divergences associated with warm interglacials and increased habitat with drop in sea level during the recent Wisconsin glaciation. Seven discrete stock management units for bull sharks are proposed.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Horizontal and vertical movements of immature dusky sharks Carcharhinus obscurus in relation to commercial longline fisheries in the western North Atlantic Ocean

Background Many species of sharks, including the dusky shark Carcharhinus obscurus, are often incidentally captured in commercial pelagic and bottom longline fisheries. Incidental capture can lead to at-vessel or post-release mortality and can be detrimental to populations of threatened, endangered, or prohibited species. The estimated at-vessel mortality for dusky sharks can be high in longline fisheries and, to minimize mortality, time-area closures have been designated in the western North Atlantic Ocean to mitigate interactions with longline fisheries, yet incidental capture of dusky sharks is still common. We compared the vertical and horizontal movements of dusky sharks to the overall fishing effort and depth fished of commercial pelagic and bottom longline fisheries to determine when and where overlap is present that could lead to incidental capture. Results Twenty-one (n = 21) dusky sharks were tagged with pop-up archival transmitting satellite tags and all were immature animals (123-200 cm FL) apart from two individuals (230 and 300 cm FL). Sharks were tagged off the coasts of North Carolina (71%) and Florida (29%). Twenty tags (95%) reported and provided tracks between 1 and 107 days (median 15 days) and 10 tags (50%) remained on sharks for > 4 days. Most individuals remained within the bottom longline closed area off the coast of North Carolina during their time at liberty. Dusky sharks primarily occupied the 20-40 m depth range 26% of the time, and overlapped with bottom and pelagic longline gears 41% and 59% of the time, respectively. Overlap was highest in the winter and spring for both commercial fisheries. Conclusions The use of archival satellite telemetry in this study has provided valuable preliminary information on vertical and horizontal movements of immature dusky sharks in western North Atlantic Ocean. Dusky sharks may be more vulnerable to incidental capture in the pelagic longline fishery due to the high fishing effort, larger areas of horizontal overlap, and greater percentage of vertical overlap. This information will inform mitigation measures of commercial longline fisheries, which can work toward population rebuilding of the species