Specific isoforms of translation initiation factor 4GI show differences in translational activity

The eukaryotic initiation factor (eIF) 4GI gene locus (eIF4GI) contains three identified promoters, generating alternately spliced mRNAs, yielding a total of five eIF4GI protein isoforms. Although eIF4GI plays a critical role in mRNA recruitment to the ribosomes, little is known about the functions of the different isoforms, their partner binding capacities, or the role of the homolog, eIF4GII, in translation initiation. To directly address this, we have used short interfering RNAs (siRNAs) expressed from DNA vectors to silence the expression of eIF4GI in HeLa cells. Here we show that reduced levels of specific mRNA and eIF4GI isoforms in HeLa cells promoted aberrant morphology and a partial inhibition of translation. The latter reflected dephosphorylation of 4E-BP1 and decreased eIF4F complex levels, with no change in eIF2 alpha phosphorylation. Expression of siRNA-resistant Myc-tagged eIF4GI isoforms has allowed us to show that the different isoforms exhibit significant differences in their ability to restore translation rates. Here we quantify the efficiency of eIF4GI promoter usage in mammalian cells and demonstrate that even though the longest isoform of eIF4GI (eIF4GIf) was relatively poorly expressed when reintroduced, it was more efficient at promoting the translation of cellular mRNAs than the more highly expressed shorter isoforms used in previous functional studies

Fate and impact of organics in an immersed membrane bioreactor applied to brine denitrification and ion exchange regeneration

The application of membrane bioreactors (MBRs) to brine denitrification for ion exchange regeneration has been studied. The developed culture was capable of complete brine denitrification at 50 gNaCl.l−1. Denitrification reduced to c.60% and c.70% when salinity was respectively increased to 75 and 100 g.l−1, presumed to be due to reduced growth rate and the low imposed solids retention time (10 days). Polysaccharide secretion was not induced by stressed cells following salt shocking, implying that cell lysis did not occur. Fouling propensity, monitored by critical flux, was steady at 12–15 l.m−2.h−1 during salinity shocking and after brine recirculation, indicating that the system was stable following perturbation. Low molecular weight polysaccharide physically adsorbed onto the nitrate selective anion exchange resin during regeneration reducing exchange capacity by c.6.5% when operating up to complete exhaustion. However, based on a breakthrough threshold of 10 mgNO3−-N.l−1 the exchange capacity was comparative to that determined when using freshly produced brine for regeneration. It was concluded that a denitrification MBR was an appropriate technology for IEX spent brine reco

Zinc calixarene complexes for the ring opening polymerization of cyclic esters

Reaction of Zn(C₆F₅)₂·toluene (two equivalents) with 1,3-dipropoxy-p-tert-butyl-calix[4]arene (L¹H₂) led to the isolation of the complex [{Zn(C₆F₅)}₂L¹] (1), whilst similar use of Zn(Me)₂ resulted in the known complex [{Zn(Me)}₂L¹] (2). Treatment of L¹H₂ with in situ prepared Zn{N(SiMe₃)₂}₂ in refluxing toluene led to the isolation of the compound [(Na)ZnN(SiMe₃)₂L¹] (3). The stepwise reaction of L¹H₂ and sodium hydride, followed by ZnCl₂ and finally NaN(SiMe₃)₂ yielded the compound [Zn{N(SiMe₃)₂}₂L¹] (4). The reaction between three equivalents of Zn(C₆F₅)₂·toluene and oxacalix[3]arene (L²H₃) at room temperature formed the compound {[Zn(C₆F₅)]₃L²} (5); heating of 5 in acetonitrile caused the ring opening of the parent oxacalix[3]arene and rearrangement to afford the complex [(L²)Zn₆(C₆F₅)(R)(RH)OH]·5MeCN R = C₆F₅CH₂-(p-ᵗBuPhenolate-CH₂OCH₂–)₂–p-ᵗBuPhenolate-CH₂O⁻)³⁻ (6). The molecular structures of the new complexes 1, 3 and 6, together with that of the known complex 2, whose solid state structure has not previously been reported, have been determined. Compounds 1, 3–5 have been screened for the ring opening polymerization (ROP) of ε-caprolactone (ε-CL) and rac-lactide. Compounds featuring a Zn–C₆F₅ fragment were found to be poor ROP pre-catalysts as they did not react with benzyl alcohol to form an alkoxide. By contrast, compound 4, which contains a zinc silylamide linkage, was the most active of the zinc-based calix[4]arene compounds screened and was capable of ROP at ambient temperature with 65% conversion over 4 h

Secondary resurfacing of the patella in total knee arthroplasty

Anterior knee pain following primary total knee arthroplasty is common and can be difficult to treat satisfactorily. We reviewed 28 consecutive patients (29 knees) who underwent secondary resurfacing of the patella for persistent anterior knee pain and report on the results. Mean follow-up was 28 months (range12-61) with no cases lost to follow-up. Oxford knee scores, range of motion, the patient's assessment of outcome and overall satisfaction were recorded. Seventeen out of 19 (59%) felt their knee was better following patellar resurfacing, 10 out of 29 (34%) felt it was the same and two out of 29 (7%) felt it was worse. There was a significant improvement in Oxford knee scores (p < 0.001) and significant increase in patient satisfaction (p < 0.001) following secondary resurfacing. While secondary resurfacing of the patella does not provide the solution for every case of anterior knee pain following total knee joint replacement, in greater than 50% of cases it can be effective at relieving symptoms and in this series carries a low risk of worsening symptoms or complications

The 2007-09 financial crisis and bank opaqueness

Doubts about the accuracy with which outside investors can assess a banking firm’s value motivate many government interventions in the banking market. The recent financial crisis has reinforced concerns about the possibility that banks are unusually opaque. Yet the empirical evidence, thus far, is mixed. This paper examines the trading characteristics of bank shares over the period from January 1990 through September 2009. We find that bank share trading exhibits sharply different features before vs. during the crisis. Until mid-2007, large (NYSE-traded) banking firms appear to be no more opaque than a set of control firms, and smaller (NASD-traded) banks are, at most, slightly more opaque. During the crisis, however, both large and small banking firms exhibit a sharp increase in opacity, consistent with the policy interventions implemented at the time. Although portfolio composition is significantly related to market microstructure variables, no specific asset category(s) stand out as particularly important in determining bank opacity.Banks and banking ; Stock market ; Financial crises

Evidence for a black hole in a radio-quiet quasar nucleus

We present the first milli-arcsecond resolution radio images of a radio-quiet quasar, detecting a high brightness temperature core with data from the VLBA. On maps made with lower-frequency data from MERLIN and the VLA jets appear to emanate from the core in opposite directions, which correspond to radio-emission on arcsecond scales seen with the VLA at higher frequencies. These provide strong evidence for a black-hole--based jet-producing central engine, rather than a starburst, being responsible for the compact radio emission in this radio-quiet quasar.Comment: 10 pages including 1 postscript figure; uses aaspp4.sty. Accepted for publication in Ap. J. Lett. Also available from http://www-astro.physics.ox.ac.uk/research/preprints

Market evidence on the opaqueness of banking firms' assets.

We assess the market microstructure properties of U.S. banking firms' equity, to determine whether they exhibit more or less evidence of asset opaqueness than similar-sized nonbanking firms. The evidence strongly indicates that large banks (traded on NASDAQ) trade much less frequently despite microstructure characteristics. Problem (noncurrent) loans tend to raise the frequency with which the bank's equity trades, as well as the equity's return volatility. The implications for regulatory policy and future market microstructure research are discussed.Bank stocks ; Bank assets

A VLT/FORS2 Multi-Slit Search for Lyman-alpha Emitting Galaxies at z~6.5

We present results from a deep spectroscopic search in the 9150A atmospheric window for z~6.5 Lyman-alpha emitting galaxies using the VLT/FORS2. Our multi-slit+narrow-band filter survey covers a total spatial area of 17.6 sq. arcmin in four different fields and reaches fluxes down to 5x10^(-18) erg/s/cm^2 (7 sigma detection). Our detection limit is significantly fainter than narrow-band searches at this redshift and fainter also than the unlensed brightness of Hu et al.'s HCM6A at z=6.56, and thus provides better overlap with surveys at much lower redshifts. Eighty secure emission line galaxies are detected. However, based on their clear continuum emission shortward of the line or the presence of multiple lines, none of these can be Ly-alpha emission at z~6.5. Our null result of finding no z~6.5 Ly-alpha emitters suggests that the number density of Ly-alpha emitters with L>2x10^(42) erg/s declines by ~2 between z~3 and z~6.5.Comment: accepted by ApJ Letters (originally submitted June 11, 2004