21 research outputs found
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Dengue virus (DENV) and its four serotypes (DENV1-
4) belong to the Flavivirus genus of the Flaviviridae family. DENV
infection is a life-threatening disease, which results in up to 20,000
deaths each year. Viruses have been shown to encode trans-regulatory
small RNAs, or microRNAs (miRNAs), which bind to messenger RNA
and negatively regulate host or viral gene expression. During DENV
infections, miRNAs interact with proteins in the RNAi pathway, and
are processed by ribonucleases such as Dicer and Drosha. This study
aims to investigate Drosha, DGCR8, and Dicer expression levels in
human A-549 cells following DENV4 infection. DENV4 infected
A-549 cells were collected daily for 5 days, and RNA was extracted
to quantify viral load. Gene expression of Drosha, Dicer, and DGCR8
was determined using quantitative PCR (RT-qPCR). We found that
DENV4 infection exhibited the highest viral load 3 days post-infection.
Dicer, Drosha, and DGCR8 showed reduced expression following
S.M.M. Casseb et al. 2
Genetics and Molecular Research 15 (2): gmr.15027891 ©FUNPEC-RP www.funpecrp.com.br
DENV4 infection as compared with negative controls. In addition,
we hypothesize that reduced expression of DGCR8 may not only be
related to miRNA biogenesis, but also other small RNAs. This study
may change our understanding regarding the relationship between host
cells and the dengue virus