2 research outputs found
Additional file 1 of Gastric point-of-care ultrasonography in patients undergoing radical gastrointestinal surgery before anesthetic induction: an observational cohort study
Supplementary Material 1
Novel Magnetic Microprobe with Benzoboroxole-Modified Flexible Multisite Arm for High-Efficiency <i>cis</i>-Diol Biomolecule Detection
With
regard to regulating a variety of biological events, including
molecular recognition, signal transduction, cell adhesion, and immune
response, <i>cis</i>-diol biomolecules, such as saccharides
and glycoproteins, play vital roles. However, saccharides and glycoproteins
in living systems usually exist in very low abundance, along with
abundant interfering components. High-efficiency detection of saccharides
and glycoproteins is a challenging yet highly impactful area of research.
Herein, we reported a novel magnetic microprobe with a benzoboroxole-modified
flexible multisite arm (PEG 2000-grafted PAMAM dendrimers; the microprobe
was denoted as BFMA-MNP) for high-efficiency saccharides detection.
The extraction capacity was significantly improved by ∼2 orders
of magnitude, because of the integration of the enhanced hydrophilicity
and multivalency effects in benzoboroxoles and the enhanced accessibility
of the binding sites within the PEG 2000-grafted PAMAM dendrimers.
As a result, the proposed approach possessed several advantages, compared
with previous boronic acid-based methods, including ultrahigh sensitivity
(limit of detection was <1 ng/mL), wide linear range (ranged from
0.5 μM to 2000 μM), and applicable in physiological pH
condition. Furthermore, we established a general BFMA-MNP/glycoproteins/AuNPs
sandwich assay to realize the visual glycoprotein qualitative screening
for the first time. The unique sandwich assay possessed the dual nature
of the magnetic separation by BFMA-MNPs and specific coloration by
citrate-coated AuNPs. This visual sandwich assay enabled fast differentiation
of the existence of glycoproteins in complicated samples without any
advanced instruments. We believe the proposed BFMA-MNP microprobe
herein will advance the ideas to detect and identify trace saccharides
and glycoproteins in important fields such as glycomics and glycoproteomics