4 research outputs found
Performance, haemato-biochemical indices and antioxidant status of growing rabbits fed on diets supplemented with Mucuna pruriens leaf meal
[EN] The effects of dietary Mucuna pruriens leaf meal (MLM) supplementation on rabbits’ performance, haemato-biochemical indices and antioxidant status outside their thermal neutrality zone (21 to 25°C) were evaluated. One hundred and twenty 35-d old crossbreed (ChinchillaĂ—New Zealand) rabbits weighing 694±5 g were allotted to 4 treatments (30 rabbits/treatment; 3 rabbits/replicate). A basal diet (crude protein: 16.9%, crude fibre: 17.6%, digestible energy: 2671 kcal/kg) was divided into 4 equal portions i.e. diets 1, 2, 3 and 4, supplemented with 0, 4, 8 and 12 g MLM/kg, respectively, and pelleted. The average body weight in rabbits fed on diets 3 and 4 was higher compared to those fed on diet 1 (control) at 91 d of age (+228 and +262 g, respectively; P=0.01). Within 35 to 91 d, the average daily weight gain in rabbits fed on diets 3 and 4 was higher compared to those fed on the control diet (+4.1and +4.8 g/d, respectively; P=0.01). The dressing-out percentage of rabbits fed on diets 3 and 4 increased (P=0.05) compared to those fed the control diet. At 63 d and 91 d of age, the white blood cell level of rabbits fed on diet 4 increased significantly compared to those fed the control diet (+5.05Ă—109 and +5.32Ă—109/L, respectively). At 63 and 91 d of age, the cholesterol level of rabbits fed on diets 3 (–1.0 and –1.16 mmol/L, respectively) and 4 (–1.10 and –1.21 mmol/L, respectively), were significantly lower compared to those fed on the control diet. The aspartate aminotransferase (AST) concentration in rabbits fed on diet 4 was reduced compared to those on control diet at 63 d of age (–33.68 IU/L; P=0.02). At 63 d and 91 d of age, compared to control, the activities of glutathione peroxidase in rabbits fed on diets 3 (+35.77 and +49.09 mg protein, respectively) and 4 (+54.52 and +55.02 mg protein, respectively) increased significantly, while catalase activities in rabbits fed diet 4 (+217.7 and +209.5 mg/g, respectively) also increased significantly. It could be concluded that dietary MLM supplementation enhanced the rabbits’ performance, reduced serum AST and cholesterol and improved the antioxidant status.Oloruntola, OD.; Ayodele, SO.; Adeyeye, SA.; Agbede, JO. (2018). Performance, haemato-biochemical indices and antioxidant status of growing rabbits fed on diets supplemented with Mucuna pruriens leaf meal. World Rabbit Science. 26(4):277-285. doi:10.4995/wrs.2018.10182SWORD277285264Adekonla A.Y., Ayo J.O. 2009. Effect of road transportation on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season in Zaria, Nigeria. J. Cell Anim. Biol., 3: 4-8.Aebi H. 1974. Catalase estimation In (ed. HV Bergmeyer). Methods of Enzymatic Analysis. Verlag Chemic, New York Academic Press, New York, US. https://doi.org/10.1016/B978-0-12-091302-2.50032-3Ansari J., Khan S.H., Hag A., Yousaf M. 2012. Effect of the level of Azadirachta indica dried leaf meal as phytogenic feed additive on growth performance and haemato-biochemical parameters in broiler chicks. J. Appl. Anim. Res., 40: 336-345. https://doi.org/10.1080/09712119.2012.692329AOAC. 1990. Official methods of analysis of the Association of Official Analytical Chemists, 15th ed. AOAC, Washington, D.C. USA.Attia Y., Bakhashwaina A.A., Bertu N.K. 2017. Thyme oil (Thyme vulgaris L) as a natural growth promoter for broiler chickens reared under hot climate. It. J. Anim. Sci., 16: 275-282. https://doi.org/10.1080/1828051X.2016.1245594Avellini L., Silverstrelli M., Gaitti A. 1995. Training-induced modifications in some biochemical defenses against free radicals in equine erythrocytes. Vet. Res. Com., 19: 179-184. https://doi.org/10.1007/BF01839296Ayodele S.O., Oloruntola O.D., Agbede J.O. 2016. Effect of Alchornea cordifolia leaf meal inclusion and enzyme supplementation on performance and digestibility of rabbits. World Rabbit Sci., 24: 201-2016. https://doi.org/10.4995/wrs.2016.3933Blasco A., Ouhayoun J., Masoero G. 1993. Harmonization of criteria and terminology in rabbit meat research. World Rabbit Sci., 1: 3-10. https://doi.org/10.4995/wrs.1993.189Brewer N.R., Cruise L.J. 1994. The Biology of the Laboratory Rabbit. 2nd Edition. In: Patrick J. Manning, Daniel H. Ringler, and Christian E. Newcomer (eds.). Elsevier Inc. pp: 483.Burnett N., Mathura K., Metivier K.S., Holder R.B., Brown G., Campbell M. 2003. An investigation into haematological and serum chemistry parameters of rabbits in Trinidad. World Rabbit Sci., 14: 175-187. https://doi.org/10.4995/wrs.2006.556Cardoso V.D.S., Lima C.A.R.D., Lima M.E.F.D., Dorneles L.E.G., Danelli M.D.G.M. 2012. Piperin as a phytogenic additive in broiler diets. Pesq. Agropec. Bras., 47: 489-496. https://doi.org/10.1590/S0100-204X2012000400003de Blas C., Mateos G.G. 2010. Feed formulation. In: Nutrition of the Rabbit. 2nd Edition (eds. C.de Blas and J. Wiseman). CAB International. The United Kingdom. pp. 229. https://doi.org/10.1079/9781845936693.0222Devi S.M., Park J.W., Kim I.H. 2015. Effect of plant extract on growth performance and insulin-like growth factor 1 secretion in growing pig. Rev. Bras. Zootec., 44: 355-360. https://doi.org/10.1590/S1806-92902015001000003Duke A.T. 1995. Handbook of Medicinal Herbs. 3rd ed. CRS Press. London, pp. 220.Edeoga G.O., Okwu D.E., Mbaebie B.O. 2005. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol., 4: 685-688. https://doi.org/10.5897/AJB2005.000-3127El-Desoky N.I., Hashem N.M., Elkomy A., Abo-Elezz Z.R. 2017. Physiological response and semen quality of rabbit buck supplemented with Moringa leaves ethanolic extract during summer season. Animal., 14: 1-9. https://doi.org/10.1017/S1751731117000088.El-Gindy Y.M., Zeweil H.S. 2017. Effects of parsley supplementation on the seminal quality, blood lipid profile and oxidant status of young and old male rabbits. World Rabbit Sci., 25: 215-223. https://doi.org/10.4995/wrs.2017.6532Ferguson NM. 1956. A textbook of pharmacology. McMillan Company. New Delhi. 191.Fernández-Carmona J., Blas E., Pascual J.J., Maertens L., Gidenne T., Xiccato G., Garcia J. 2005. Recommendations and guidelines for applied nutrition experiments in rabbits. World Rabbit Sci., 13: 209-228. https://doi.org/10.4995/wrs.2005.516Gyamfi M.A., Yonamine M., Aaniya Y. 1999. Free radical scavenging action of medicinal herbs from Ghana: Thonningia sanguine on experimentally induced liver injuries. General Pharmacol., 32: 661-667. https://doi.org/10.1016/S0306-3623(98)00238-9Halliwell B.E., Gutteridge J.M.C. 1989. Lipid Peroxidation: a Radical Chain Reaction, Free Radical in Biology and Medicine, 2nd ed. Oxford University Press, New York, NY, 188-218.Harborne J.B. 1973. Phytochemical methods, Chapman and Hall Ltd, London, 49-188 pp.Jiang Z Y., Jiang SQ., Lin YC, Zi PB, Yu DQ, Wu TX. 2007. Effect of soybean isoflavone on growth performance, meat quality and antioxidation in male broiler. Poultry Sci., 86: 1356-1362. https://doi.org/10.1093/ps/86.7.1356Jimoh A.O., Ewuola E.O., Balogun A.S. 2017. Oxidative stress marker in exotic breeds of rabbits during peak of heat stress in Ibadan, Nigeria. J. Adv. Bio. Biotech., 12: 1-9. https://doi.org/10.9734/JABB/2017/30437Kamel C. 2000. Natural plant extracts: Clinical remedies bring modern animal production solutions. In: 3rd Conference on sow feed manufacturing in the Mediterranean Region. 31-38.Konca Y., Kirkpinar F., Mert S., Yurtseven S. 2009. Effect of dietary ascorbic acid supplementation on growth performance, carcass, bone quality and blood parameters in broilers during natural summer temperature. Asian J. Anim.Vet. Advs., 4: 139-147. https://doi.org/10.3923/ajava.2009.139.147Kone A.P., Cinq-Mars D., Desjardins Y., Guay F., Gosselin A., Saucier L. 2016. Effects of plant extracts and essential oils as feed supplements on quality and microbial traits of rabbit meat. World Rabbit Sci., 24: 107-119. https://doi.org/10.4995/wrs.2016.3665Kumar A., Rajput G., Dhatwalia V.K., Srivastav G. 2009. Phytocontent screening of Mucuna seeds and exploit in opposition to pathogenic microbes. J. Biol. Environ. Sci., 3: 71-76.Kumar D.S., Muthu A.K. 2010. Free radical scavenging activity of various extracts of whole plant of Mucuna pruriens (Linn): An in-vitro evaluation. J. Pharm. Res., 3: 718-721.Kumar S., Kumar A.B.V., Meena K. 2011. Review: Effect of heat stress in tropical livestock and different strategies for its amelioration. J. Stress Physiol. Biochem., 7: 45-54.Li S., Zhao M., Jiang T., Lv W., Gao S., Zhou Y., Miao Z. 2018. Growth performance and antioxidant status of growing rabbits fed on diets supplemented with Eucommia ulmoides leaves. World Rabbit Sci., 26: 35-41. https://doi.org/10.4995/wrs.2018.7864Liu H.W., Dong X.F., Tong J.M., Qi Z. 2010. Alfalfa polysaccharides improve the growth performance and antioxidant status of heat-stressed rabbits. Livest. Sci., 131: 88-93. https://doi.org/10.1016/j.livsci.2010.03.004Lording P.M., Friend S.C.E. 1991. Interpretation of laboratory results. Aust. Vet. Pract., 21: 188-192.Marai I.F.M., Habeeb A.A.M., Gad A.E. 2002. Rabbit's productive, reproductive and physiological performance traits as affected by heat stress: A review. Livest. Prod. Sci., 78: 71-90. https://doi.org/10.1016/S0301-6226(02)00091-XMarco-JimĂ©nez F., GarcĂa-Diego F.J., Vicente J.S. 2017. Effect of gestational and lactational exposure to heat stress on performance in rabbits. World Rabbit Sci., 25: 17-25. https://doi.org/10.4995/wrs.2017.5728Masella R., Benedetto R.D., Varì R., Filesi C., Giovannini C. 2005. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J. Nutr. Biochem., 16: 577-586. https://doi.org/10.1016/j.jnutbio.2005.05.013Meineri G., Giacobini M., Forneris G. 2017. Evaluation of physiological parameters of the plasma oxidative status in rabbits. J. Appl. Anim. Res., 45: 315-319. https://doi.org/10.1080/09712119.2016.1190734Mertens D.R. 2002. Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing in breakers of crucibles: Collaborative study. J. AOAC. Int., 85: 12187-1240.Misra, H.P., Fridovich, I. 1972. The univalent reduction of oxygen by flavins and quinines. J. Biol. Chem., 247: 188-192.Muanda F., Kone D., Dicko A., Soulimani R., Younos C. 2011. Phytochemical composition and antioxidant capacity of three Malian medicinal plant parts. J. Evid. Based Complementary Altern. Med., 2011: 21-28. https://doi.org/10.1093/ecam/nep109Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3Oloruntola O.D., Ayodele S.O., Agbede J.O, Oloruntola D.A, Ogunsipe M.H., Omoniyi I.S. 2016a. Effect of Alchornea cordifolia leaf meal and enzyme supplementation on growth, haematological, immunostimulatory and serum biochemical response of rabbits. Asian J. Biol. Life Sci., 5: 190-195.Oloruntola O.D., Ayodele S.O., Agbede J.O., Oloruntola D.A. 2016b. Effect of feeding broiler chicken with diets containing Alchornea cordifolia leaf meal and enzyme supplementation. Arch. Zootec., 65: 489-498.Pulido R., Bravo L., Saura-Calixto F. 2002. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J. Agric Food Chem., 48: 3396-3402. https://doi.org/10.1021/jf9913458Robertson J.B., Van Soest P.J. 1981. The detergent system of analysis. In: James W.P.T., Theander O. (eds.) The analysis of dietary fibre in food. Marcel Dekker, New York. 123-159.Rotruck J.T., Pope A.L., Ganther H.E., Hafeman D.G., Hoekstra W.G. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science, 179: 588-590. https://doi.org/10.1126/science.179.4073.588Shad M.A., Nawaz H., Rehma T., Ikram M., 2013. Determination of biochemicals, phytochemicals and antioxidative properties of different part of Cichorium intybus L.: A comparative study. The J. Anim. Plant Sci., 23: 1060-1066.Sies H. 1997. Oxidative stress: oxidants and antioxidants, Exp. Physiol., 82: 291-295. https://doi.org/10.1113/expphysiol.1997.sp004024Suresh S., Prithiviraj E., Lakshmi N.V., Ganesh M.K., Ganesh L., Prakash S. 2013. Effect of Mucuna pruriens (Linn.) on mitochondrial dysfunction and DNA damage in epididymal sperm of streptozotocin induced diabetic rat. J. Ethnopharmacol., 145: 32-41. https://doi.org/10.1016/j.jep.2012.10.030Tawfeek S.S., Hassanin K.M.A., Youssef I.M.I. 2014. The effect of dietary supplementation of some antioxidants on performance, oxidative stress, and blood parameters in broilers under natural summer conditions. J. World's Poult. Res., 4: 10-19.Valenzuela-Grijalva N.V., Pinelli-Saavedra A., Muhlia-Almazan A., DomĂnguez-DĂaz D., González-RĂos H. 2017. Dietary inclusion effects of phytochemicals as growth promoters in animal production. J. Anim. Sci. Tech., 58: 8. https://doi.org/10.1186/s40781-017-0133-9Van-Burden T.P., Robinson W.C., 1981. Formation of complexes between protein and Tannin acid. J. Agric. Food Chem. 1: 77.Xiccato G., Trocino A. 2010. Energy and protein metabolism and requirements. In: C. de Blass, J. Wiseman (eds.).Nutrition of the rabbit, 2nd edition. CAB International, United Kingdom. pp. 83. https://doi.org/10.1079/9781845936693.0083Yadav M.K., Upadhyay P., Purohit S., Pandey B.L. Shah H. 2017. Phytochemistry and pharmacological activity of Mucuna pruriens: A review. Int. J. Green Pharm., 11: 69-73. https://doi.org/10.22377/ijgp.v11i02.916Yilkal T. 2015. Important anti-nutritional substances and inherent toxicants of feeds. Food Sci. Quality Man., 36: 40-47.Yuan S., Chen D., Zhang K., Yu B. 2007. Effect of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weaning pigs. Asian-Aust. J. Anim. Sci., 20: 1600-1605.Zeweil H.S., Elgindy Y.M. 2016. Pomegranate peel as a natural antioxidant enhanced reproductive performance and milk yield of female rabbits. World Rabbit Sci., 24: 207-212. https://doi.org/10.4995/wrs.2016.402
Processed cocoa pod husk dietary inclusion: effects on the performance, carcass, haematogram, biochemical indices, antioxidant enzyme and histology of the liver and kidney in broiler chicken
Abstract Background In a 42-day feeding trial, the effects of processed cocoa pod husk (PCHM) inclusion in a broiler chicken diet were assessed. Methods This experiment was conducted between December 2017 and January 2018. Cocoa pod husk was collected and processed by ash treatment and rumen liquor fermentation to form a processed cocoa pod husk (PCHM). Three experimental diets were formulated at both the starter and finisher phases, in which PCHM was included at 0, 4 and 8% and designated as diets 1, 2 and 3, respectively. One hundred and eighty 1-day-old Arbor Acres broiler chicks were randomly distributed to three dietary treatments (10 birds/replicate; 60 birds/treatment) in a completely randomized design. The growth performance, carcass, relative internal organ weights and haemato-biochemical indices were determined. Histological examination of the liver and heart samples was also determined. Results The PCHM inclusion did not affect (P > 0.05) the performance characteristics of the broiler chicks, except for the feed intake that significantly (P  0.05) across the dietary treatments. The serum glutathione peroxidase and catalase concentration were higher (P < 0.05) in birds fed PCHM-inclusive diets compared to those fed the control diet. Similar histological myocardiac cell appearances were observed among the birds across the various dietary treatments. Sections show the myocardium composed of the cardiac muscle with peripherally placed nucleus separated by a defined interstitium that is free of inflammatory cells and collections. In the birds fed diet 2 and 3, histological variations observed were marked vascular congestion and perivascular inflammatory cells infiltrations in the hepatic tissue and marked infiltration of polymorphonuclear cells around the vessels and activation of hepatic macrophage: Kupffer cells. Conclusion Dietary PCHM inclusion up to 8% supports the performance, stability of haemato-biochemical indices and improved antioxidant status of the broiler chickens under heat stress condition. Histological changes were observed in the broiler chicken liver
Effect of pawpaw leaf meal and multi-enzyme supplementation in the diet on performance, digestibility, and oxidative enzyme status of rabbits
Abstract Background This study aimed at determining the effect of pawpaw leaf meal (PLM 26.14% crude protein [CP] and 16.06% crude fiber [CF]) and multi-enzyme (m-E: amylase, beta-glucanase, phytase, cellulase, and xylanase, protease) supplementation in diets on performance, nutrient digestibility, and oxidative status of rabbits. Four experimental diets were formulated and designated as diet 1(0%PLM-m-E), diet 2(0%PLM + m-E), diet 3(10%PLM-m-E), and diet 4(10%PLM + m-E). One hundred and twenty (120), 5-week-old rabbits of cross breed rabbits (Chinchilla x New-Zealand white) of equal sexes; weighing 738.21 ± 33 g were randomly distributed to 4 dietary treatments (30 rabbits/treatment; 3 rabbits/replicate). Results Highest weight gain being recorded in rabbits fed diet 4 is similar to the weight gain recorded for rabbits fed diets 2 and 3 but higher (P < 0.05) than weight gain of rabbits fed diet 1. Best feed conversion ratio (FCR) recorded in rabbits fed diet 4 was comparable to the FCR of rabbits fed diets 2 and 3 but higher (P < 0.05) than FCR of rabbits fed diet 1. Higher CP digestibility recorded in rabbits fed diets 2 and 4 were similar to CP digestibility in rabbits fed diet 3 but higher (P = 0.01) than CP digestibility in rabbits fed with diet 1. The starch digestibility was higher (P < 0.05) in the rabbits fed m-E supplemented diets 2 and 4. Higher (P < 0.05) glutathione peroxidase and superoxide dismutase activities were recorded in rabbits fed with diets 3 and 4. Conclusion PLM inclusion and m-E supplementation improved performance, crude protein, and starch digestibility and antioxidant enzyme status of the rabbits