The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression. © 2011 European Molecular Biology Organization

Tests of the Las Campanas Distant Cluster Survey from Confirmation Observations for the ESO Distant Cluster Survey

The ESO Distant Cluster Survey (EDisCS) is a photometric and spectroscopic study of the galaxy cluster population at two epochs, z~0.5 and z~0.8, drawn from the Las Campanas Distant Cluster Survey (LCDCS). We report results from the initial candidate confirmation stage of the program and use these results to probe the properties of the LCDCS. Of the 30 candidates targeted, we find statistically significant overdensities of red galaxies near 28. Of the ten additional candidates serendipitously observed within the fields of the targeted 30, we detect red galaxy overdensities near six. We test the robustness of the published LCDCS estimated redshifts to misidentification of the brighest cluster galaxy (BCG) in the survey data, and measure the spatial alignment of the published cluster coordinates, the peak red galaxy overdensity, and the brightest cluster galaxy. We conclude that for LCDCS clusters out to z~0.8, 1) the LCDCS coordinates agree with the centroid of the red galaxy overdensity to within 25'' (~150 h^{-1} kpc) for 34 out of 37 candidates with 3\sigma galaxy overdensities, 2) BCGs are typically coincident with the centroid of the red galaxy population to within a projected separation of 200 h^{-1} kpc (32 out of 34 confirmed candidates), 3) the red galaxy population is strongly concentrated, and 4) the misidentification of the BCG in the LCDCS causes a redshift error >0.1 in 15-20% of the LCDCS candidates. These findings together help explain the success of the surface brightness fluctuations detection method.Comment: 10 pages, 9 figures, accepted for publication in the November 10 issue of Ap

The Galaxy Populations of X-Ray Detected, Poor Groups

(Abridged) We determine the quantitative morphology and star formation properties of galaxies in six nearby X-ray detected, poor groups using multi-object spectroscopy and wide-field R imaging. We measure structural parameters for each galaxy by fitting a PSF-convolved, two component model to their surface brightness profiles. To compare directly the samples, we fade, smooth, and rebin each galaxy image so that we effectively observe each galaxy at the same redshift (9000 km/s) and physical resolution (0.87h^(-1) kpc). We compare results for the groups to a sample of field galaxies. We find that: 1) Galaxies spanning a wide range in morphological type and luminosity are well-fit by a de Vaucouleurs bulge with exponential disk profile. 2) Morphologically classifying these nearby group galaxies by their bulge fraction (B/T) is fairly robust on average, even when their redshift has increased by up to a factor of four and the effective resolution of the images is degraded by up to a factor of five. 3) The fraction of bulge-dominated systems in these groups is higher than in the field (~50% vs. ~20%). 4) The fraction of bulge-dominated systems in groups decreases with increasing radius, similar to the morphology-radius (~density) relation observed in galaxy clusters. 5) Current star formation in group galaxies is correlated with significant morphological asymmetry for disk-dominated systems (B/T<0.4). 6) The group galaxies that are most disk-dominated (B/T<0.2) are less star forming and asymmetric on average than their counterparts in the field.Comment: Accepted for publication in the Astrophysical Journal (26 pages + 12 figures); Figs 1 & 2 also available at http://www.ucolick.org/~vy/astronomy/groups_figs.tar.g

The SBF Survey of Galaxy Distances. I. Sample Selection, Photometric Calibration, and the Hubble Constant

We describe a program of surface brightness fluctuation (SBF) measurements for determining galaxy distances. This paper presents the photometric calibration of our sample and of SBF in general. Basing our zero point on observations of Cepheid variable stars, we find that the absolute SBF magnitude in the Kron-Cousins I band correlates well with the mean (V-I)o color of a galaxy according to M_Ibar = (-1.74 +/- 0.07) + (4.5 +/- 0.25) [ (V-I)o - 1.15 ] for 1.0 < (V-I) < 1.3. This agrees well with theoretical estimates from stellar population models. Comparisons between SBF distances and a variety of other estimators, including Cepheid variable stars, the Planetary Nebula Luminosity Function (PNLF), Tully-Fisher (TF), Dn-sigma, SNII, and SNIa, demonstrate that the calibration of SBF is universally valid and that SBF error estimates are accurate. The zero point given by Cepheids, PNLF, TF (both calibrated using Cepheids), and SNII is in units of Mpc; the zero point given by TF (referenced to a distant frame), Dn-sigma and SNIa is in terms of a Hubble expansion velocity expressed in km/s. Tying together these two zero points yields a Hubble constant of H_0 = 81 +/- 6 km/s/Mpc. As part of this analysis, we present SBF distances to 12 nearby groups of galaxies where Cepheids, SNII, and SNIa have been observed.Comment: 29 pages plus 8 figures; LaTeX (AASTeX) uses aaspp4.sty (included); To appear in The Astrophysical Journal, 1997 February 1 issue; Compressed PostScript available from ftp://mars.tuc.noao.edu/sbf

Steady-State Contrast Response Functions Provide a Sensitive and Objective Index of Amblyopic Deficits

Purpose. Visual deficits in amblyopia are neural in origin, yet are difficult to characterize with functional magnetic resonance imagery (fMRI). Our aim was to develop an objective electroencephalography (EEG) paradigm that can be used to provide a clinically useful index of amblyopic deficits. Methods. We used steady-state visual evoked potentials (SSVEPs) to measure full contrast response functions in both amblyopic (n = 10, strabismic or mixed amblyopia, mean age: 44 years) and control (n = 5, mean age: 31 years) observers, both with and without a dichoptic mask. Results. At the highest target contrast, the ratio of amplitudes across the weaker and stronger eyes was highly correlated (r = 0.76) with the acuity ratio between the eyes. We also found that the contrast response function in the amblyopic eye had both a greatly reduced amplitude and a shallower slope, but that surprisingly dichoptic masking was weaker than in controls. The results were compared with the predictions of a computational model of amblyopia and suggest a modification to the model whereby excitatory (but not suppressive) signals are attenuated in the amblyopic eye. Conclusions. We suggest that SSVEPs offer a sensitive and objective measure of the ocular imbalance in amblyopia and could be used to assess the efficacy of amblyopia therapies currently under development

Recovering 3D structural properties of galaxies from SDSS-like photometry

Because of the 3D nature of galaxies, an algorithm for constructing spatial density distribution models of galaxies on the basis of galaxy images has many advantages over surface density distribution approximations. We present a method for deriving spatial structure and overall parameters of galaxies from images and estimate its accuracy and derived parameter degeneracies on a sample of idealised model galaxies. The test galaxies consist of a disc-like component and a spheroidal component with varying proportions and properties. Both components are assumed to be axially symmetric and coplanar. We simulate these test galaxies as if observed in the SDSS project through ugriz filters, thus gaining a set of realistically imperfect images of galaxies with known intrinsic properties. These artificial SDSS galaxies were thereafter remodelled by approximating the surface brightness distribution with a 2D projection of a bulge+disc spatial distribution model and the restored parameters were compared to the initial ones. Down to the r-band limiting magnitude 18, errors of the restored integral luminosities and colour indices remain within 0.05 mag and errors of the luminosities of individual components within 0.2 mag. Accuracy of the restored bulge-to-disc ratios (B/D) is within 40% in most cases, and becomes worse for galaxies with low B/D, but the general balance between bulges and discs is not shifted systematically. Assuming that the intrinsic disc axial ratio is < 0.3, the inclination angles can be estimated with errors < 5deg for most of the galaxies with B/D < 2 and with errors < 15deg up to B/D = 6. Errors of the recovered sizes of the galactic components are below 10% in most cases. In general, models of disc components are more accurate than models of spheroidal components for geometrical reasons.Comment: 15 pages, 13 figures, accepted for publication in RA