47 research outputs found
Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods
The aim of this study was to compare two nucleic acid extraction
methods for the recovery of enteric viruses from activated sludge. Test
samples were inoculated with human adenovirus (AdV), hepatitis A virus
(HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an
adsorption-elution-precipitation method. Two extraction methods were
used: an organic solvent-based method and a silica method. The
organic-based method was able to recoup 20% of the AdV, 90% of the RV
and 100% of both the PV and HAV from seeded samples. The silica method
was able to recoup 1.8% of the AdV and 90% of the RV. These results
indicate that the organic-based method is more suitable for detecting
viruses in sewage sludge