Revealed results of PCR in 2% agarose gel stained with ethidium bromide under UV.

<p><i>Above:</i> Samples from sixteen tsetse flies submitted to PCR using GmTub primers as DNA quality control. Expected band size ∼380 bp. <i>Middle:</i> Molecular diagnosis of samples from fifteen tsetse flies for <i>T. b. gambiense</i>. First sample corresponds to the one positive tsetse fly. The band at right is the positive control. Expected band size 270 bp. <i>Below:</i> Detection of <i>T. brucei</i> s.l. in animal samples. 5–8 and 11 are positives, 12 is the positive control. Expected band size 177 bp.</p

Distribution of animal sampling and infection amongst villages.

<p>Although three sheep were censed none of them could be sampled. In Musola, Rilaja, Bombe, Moeri and Patio Mora, no animals were present.</p

Distribution of tsetse fly captures and infection amongst villages.

<p>No flies were trapped in Moeri or Patio Mora.</p><p>*AD = Apparent density calculated as number of flies/trap/day.</p><p>**Boloco belongs to Fortuny village but a set of five traps were located there due to its relative geographical distance.</p

Map of Luba focus and distribution of tsetse fly captures over the villages.

<p>Map of Luba focus and distribution of tsetse fly captures over the villages.</p