12 research outputs found
Intoxicação por ervilhaca (Vicia villosa) em bovinos : caracterização das lesões cutâneas
Ervilhaca (Vicia spp.) é uma forrageira cuja ingestão pode acarretar em um quadro clínico conhecido como doença granulomatosa sistêmica. A espécie comumente relacionada a casos de intoxicação é a Vicia villosa. O objetivo é descrever lesões mascroscópicas e microscópicas e tipo celular envolvido na resposta de bovinos com doença granulomatosa sistêmica. Um estudo retropectivo foi realizado nos arquivos do setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (UFRGS) no período de 2005 – 2016 afim de localizar amostras de pele de bovinos com doença granulomatosa sistêmica associada à ingestão de ervilhaca. Os dados levantados foram sexo, idade e raça. Laudos de necropsia e fotografia dos casos foram consultados para analisar as lesões macroscópicas, suas localizações e frequência de aparecimento. Blocos de parafina contendo amostras de pele foram cortados e corados pela técnica de hematoxilina e eosina (HE). Realizou-se testes imuno-histoquímicos para os anticorpos anti-CD79a (linfócitos B), anti-CD3 (linfócitos T) e anti-CD68 (macrófagos). As lesões macrosocopicas, microscopicaa e a imuno-marcação foram classificadas em leve (+), moderada (++) e acentuada (+++). Em 8 animais diagnosticados com doença granulomatosa sistêmica relacionada ao consumo de Vicia spp, todos eram fêmeas com idade entre 5 e 8 anos (média de 6,6 anos), das raças Holandesa e Jersey. Observou-se alopecia (8/8) e crostas (7/8). As lesões macroscópicas estavam assim distribuídas: cabeça (7/8), membros (5/8). O infiltrado inflamatório variou de leve a moderado (7/7) e foi classificado em: dermatite perivascular leve a acentuada (7/7), perifoliculite leve a moderada (4/7) e estava composto por linfócitos (7/7), macrófagos (7/7) e ocasionais eosinófilos (4/7). Constatou-se também ectasia de glândulas sudoríparas moderada (7/7) e hiperqueratose ortoqueratótica leve a moderada (6/7). Na imuno-histoquímica, foram marcados linfócitos T (CD3) levemente a acentuadamente, macrófagos (CD68) marcando discretamente e os linfócitos B (CD79a) nos quais houve marcações raras ou negativas. Embora haja relato da intoxicação por Vicia villosa em bovinos de raças destinadas à produção de carne, nesse estudo os animais acometidos eram de raças destinados à bovinocultura de leite. As lesões de pele da doença granulomatosa sistêmica pelo consumo de ervilhaca foram observadas basicamente na cabeça, pescoço, tronco e membros. As lesões histológicas mais frequentes foram dermatite perivascular e perfoliculite com infiltrado inflamatório constituído por linfócitos, macrófagos, ocasionalmente eosinófilos e células gigantes multinucleadas. A imuno-histoquímica mostrou que os linfócitos foram as células mais marcadas.Hairy vetch (Vicia spp.) is a forage whose ingestion can cause a clinical conditon known by systemic granulomatous disease. Vicia villosa is the specie of hairy vetch related to systemic granulomatous disease in cattle. A retrospective study of necropsy and biopsy exams performed between the period of 2005-2016 aiming for cattle with systemic granulomatous disease after consumption of hairy vetch was carried out in the archives of the Setor de Patologia Veterinária from the UFRGS. The data collected were sex, age and breed. Necropsy, reports and photographs of the cases were consulted to analyze macroscopic lesions, their locations and frequency of appearance. Paraffin blocks containing skin samples were cut and stained using hematoxylin and eosin (HE). Polymer immunohistochemical assays were also performed for anti-CD79a (B lymphocytes), anti-CD3 (T lymphocytes) and anti-CD68 (macrophages). Gross and microscopy lesions and immuno-marking were quantified in mild (+), moderate (++), and severe (+++). The diagnosis of systemic granulomatous disease with skin lesions after consumption of Vicia villosa was observed in eight cattle. All animals were females, with 5-8 year-old (average 6.6 years), Holstein Friesian cattle (7) and Jersey (1) breeds. Grossly, lesions were characterized by alopecia (8/8), crusts (7/8) and involved the head (7/8) and limbs (5/8). Histology revealed a mild to moderate inflammatory infiltrate (7/7), composed by lymphocytes (7/7), macrophages (7/7), occasional eosinophils, and rare multinucleated giant cells (1/7). It was also classified in mild to severe perivascular dermatitis (7/7), mild to moderate perifolliculitis (4/7). Another findings were moderate apocrine sweat gland ectasia (7/7) and mild to moderate orthokeratotic hyperkeratosis (6/7). Immunohistochemistry of all skin sections had mild to severe staining for T cell (CD3), mild staining for macrophages (CD68), and immunostaining was rare (4/7) or absent (3/7) for B cells (CD79a). Although there is a report of the occurrence of Vicia villosa poisoning in beef cattle, in the present study the affected animals were dairy cattle. Most of the skin lesions of systemic granulomatous disease due to the consumption of vetch were observed more frequently in the head, neck, trunk and limbs. Histologically, these consisted of perivascular dermatitis, and perifolliculitis with an inflammatory infiltrate composed predominantly by lymphocytes, macrophages, with occasional eosinophils and multinucleated giant cells. Immunohistochemistry demonstrates a marked immunostaining for T cells
Efeito do feijão guandu como fonte de tanino condensado sobre a contagem de ovos por grama de fezes (OPG) de cordeiros terminados em pastagem tropical no outono
Lesions in the skin of cattle associated to hairy vetch consumption (Vicia villosa)
Background: Hairy vetch (Vicia spp.) is a high-protein source forage to cattle. The poisoning is clinically characterized by a systemic granulomatous disease, which causes dermatitis, diarrhea, decreased milk production and weight loss. The specie of hairy vetch related to systemic granulomatous disease in cattle is Vicia villosa. This work aims to describe the epidemiological, clinical, gross, microscopic and immunohistochemistry features of the skin lesions caused by the consumption of V. villosa in cattle affected by the systemic granulomatous disease. Materials, Methods & Results: A retrospective study of necropsy and biopsy exams performed between the period of 2005-2016 aiming for cattle with systemic granulomatous disease after consumption of hairy vetch was carried out in the archives of the Setor de Patologia Veterinária from the UFRGS. Epidemiological data included the sex, age, and breed of the animals affected. Gross and microscopical lesions, in addition to the immunohistochemistry anti-T lymphocytes (CD3), anti-B lymphocytes (CD79a), and anti-macrophages (CD68) features, were evaluated. The histological lesions and immunohistochemistry staining were quantified in mild (+), moderate (++), and severe (+++). The diagnosis of systemic granulomatous disease with skin lesions after consumption of Vicia villosa was observed in eight cattle. All animals were females, with 5-8 year-old (average 6.6 years), Holstein Friesian cattle (7) and Jersey (1) breeds. These cattle had a clinical history of severe pruritus, anorexia, apathy, decreased milk production, weight loss, and hyperthermia. Grossly, lesions were characterized by alopecia (8/8), crusts (7/8), lichenification and seborrhea (2/8), and exudative lesions (2/8), and involved the head (7/8), limbs (5/8), neck (4/8), trunk (4/8), perineum area (3/8), udder (3/8), and tail (3/8) Histology revealed a mild to moderate inflammatory infiltrate (7/7), composed by lymphocytes (7/7), macrophages (7/7), occasional eosinophils, and rare multinucleated giant cells (1/7). It was also classified in mild to severe perivascular dermatitis (7/7), mild to moderate perifolliculitis (4/7), superficial dermatitis (3/7), moderate to severe mural folliculitis (2/7), and hidradenitis (1/7). Another findings were moderate apocrine sweat gland ectasia, mild to moderate orthokeratotic hyperkeratosis, mild to moderate spongiosis, mild to moderate acanthosis, mild to severe serocellular crusts, mild pigmentary incontinence, mild to severe Munro’s microabscesses, mild to moderate hydropic degeneration, apoptosis, ulcers, mild to moderate superficial edema of the dermis, variable epitheliotropism and erosions. Immunohistochemistry of all skin sections had mild to severe staining for T cell (CD3), mild staining for macrophages (CD68), and immunostaining was rare (4/7) or absent (3/7) for B cells (CD79a). Discussion: Cutaneous lesions observed in this study were similar to those previously described by other authors for this condition, and were characterized by focal to coalescent areas of alopecia, lichenification and seborrhea, associated clinically to a severe pruritus. Histologically, these consisted of perivascular dermatitis, and perifolliculitis with an inflammatory infiltrate composed predominantly by lymphocytes, macrophages, with occasional eosinophils and multinucleated giant cells. Immunohistochemistry demonstrates a marked immunostaining for T cells, while it was mild for macrophages, which reinforces the involvement of the delayed type hypersensitivity (type IV) reaction in the pathogenesis of the disease
DESENVOLVIMENTO DE UM SISTEMA ANESTÉSICO CONVERSÍVEL CIRCULAR PARA TO-AND-FRO (NÃO VALVULAR) PARA ANIMAIS DE GRANDE PORTE
Efeito do feijão guandu como fonte de tanino condensado sobre a contagem de ovos por grama de fezes (OPG) de cordeiros terminados em pastagem tropical no outono
El magisterio español : Revista General de la Enseñanza: Epoca 4ª Año LV Número 6056 - 1921 Agosto 02
Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200
DESENVOLVIMENTO DE UM SISTEMA ANESTÉSICO CONVERSÍVEL CIRCULAR PARA TO-AND-FRO (NÃO VALVULAR) PARA ANIMAIS DE GRANDE PORTE
Intoxicação por ervilhaca (Vicia villosa) em bovinos : caracterização das lesões cutâneas
Ervilhaca (Vicia spp.) é uma forrageira cuja ingestão pode acarretar em um quadro clínico conhecido como doença granulomatosa sistêmica. A espécie comumente relacionada a casos de intoxicação é a Vicia villosa. O objetivo é descrever lesões mascroscópicas e microscópicas e tipo celular envolvido na resposta de bovinos com doença granulomatosa sistêmica. Um estudo retropectivo foi realizado nos arquivos do setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (UFRGS) no período de 2005 – 2016 afim de localizar amostras de pele de bovinos com doença granulomatosa sistêmica associada à ingestão de ervilhaca. Os dados levantados foram sexo, idade e raça. Laudos de necropsia e fotografia dos casos foram consultados para analisar as lesões macroscópicas, suas localizações e frequência de aparecimento. Blocos de parafina contendo amostras de pele foram cortados e corados pela técnica de hematoxilina e eosina (HE). Realizou-se testes imuno-histoquímicos para os anticorpos anti-CD79a (linfócitos B), anti-CD3 (linfócitos T) e anti-CD68 (macrófagos). As lesões macrosocopicas, microscopicaa e a imuno-marcação foram classificadas em leve (+), moderada (++) e acentuada (+++). Em 8 animais diagnosticados com doença granulomatosa sistêmica relacionada ao consumo de Vicia spp, todos eram fêmeas com idade entre 5 e 8 anos (média de 6,6 anos), das raças Holandesa e Jersey. Observou-se alopecia (8/8) e crostas (7/8). As lesões macroscópicas estavam assim distribuídas: cabeça (7/8), membros (5/8). O infiltrado inflamatório variou de leve a moderado (7/7) e foi classificado em: dermatite perivascular leve a acentuada (7/7), perifoliculite leve a moderada (4/7) e estava composto por linfócitos (7/7), macrófagos (7/7) e ocasionais eosinófilos (4/7). Constatou-se também ectasia de glândulas sudoríparas moderada (7/7) e hiperqueratose ortoqueratótica leve a moderada (6/7). Na imuno-histoquímica, foram marcados linfócitos T (CD3) levemente a acentuadamente, macrófagos (CD68) marcando discretamente e os linfócitos B (CD79a) nos quais houve marcações raras ou negativas. Embora haja relato da intoxicação por Vicia villosa em bovinos de raças destinadas à produção de carne, nesse estudo os animais acometidos eram de raças destinados à bovinocultura de leite. As lesões de pele da doença granulomatosa sistêmica pelo consumo de ervilhaca foram observadas basicamente na cabeça, pescoço, tronco e membros. As lesões histológicas mais frequentes foram dermatite perivascular e perfoliculite com infiltrado inflamatório constituído por linfócitos, macrófagos, ocasionalmente eosinófilos e células gigantes multinucleadas. A imuno-histoquímica mostrou que os linfócitos foram as células mais marcadas.Hairy vetch (Vicia spp.) is a forage whose ingestion can cause a clinical conditon known by systemic granulomatous disease. Vicia villosa is the specie of hairy vetch related to systemic granulomatous disease in cattle. A retrospective study of necropsy and biopsy exams performed between the period of 2005-2016 aiming for cattle with systemic granulomatous disease after consumption of hairy vetch was carried out in the archives of the Setor de Patologia Veterinária from the UFRGS. The data collected were sex, age and breed. Necropsy, reports and photographs of the cases were consulted to analyze macroscopic lesions, their locations and frequency of appearance. Paraffin blocks containing skin samples were cut and stained using hematoxylin and eosin (HE). Polymer immunohistochemical assays were also performed for anti-CD79a (B lymphocytes), anti-CD3 (T lymphocytes) and anti-CD68 (macrophages). Gross and microscopy lesions and immuno-marking were quantified in mild (+), moderate (++), and severe (+++). The diagnosis of systemic granulomatous disease with skin lesions after consumption of Vicia villosa was observed in eight cattle. All animals were females, with 5-8 year-old (average 6.6 years), Holstein Friesian cattle (7) and Jersey (1) breeds. Grossly, lesions were characterized by alopecia (8/8), crusts (7/8) and involved the head (7/8) and limbs (5/8). Histology revealed a mild to moderate inflammatory infiltrate (7/7), composed by lymphocytes (7/7), macrophages (7/7), occasional eosinophils, and rare multinucleated giant cells (1/7). It was also classified in mild to severe perivascular dermatitis (7/7), mild to moderate perifolliculitis (4/7). Another findings were moderate apocrine sweat gland ectasia (7/7) and mild to moderate orthokeratotic hyperkeratosis (6/7). Immunohistochemistry of all skin sections had mild to severe staining for T cell (CD3), mild staining for macrophages (CD68), and immunostaining was rare (4/7) or absent (3/7) for B cells (CD79a). Although there is a report of the occurrence of Vicia villosa poisoning in beef cattle, in the present study the affected animals were dairy cattle. Most of the skin lesions of systemic granulomatous disease due to the consumption of vetch were observed more frequently in the head, neck, trunk and limbs. Histologically, these consisted of perivascular dermatitis, and perifolliculitis with an inflammatory infiltrate composed predominantly by lymphocytes, macrophages, with occasional eosinophils and multinucleated giant cells. Immunohistochemistry demonstrates a marked immunostaining for T cells