Schematic drawing of mBSA-induced arthritis and Col V oral administration for 30 days.

<p>Schematic drawing of mBSA-induced arthritis and Col V oral administration for 30 days.</p

Histological sections of synovial tissue from the IA, IA/Suppl and Suppl groups.

<p>Panel (A and B) shows synovial tissue from the Suppl group with vessels and synovial membrane preserved (arrowhead) and scarce inflammatory cells (arrow). The arthritis group (Panel A and B) has prominent edema, synovial membrane inflammation (arrowhead), cellular inflammatory infiltrate and fibrotic thickening (arrow). In contrast, the IA/Suppl (A and B) groups showed significant reductions in edema, inflammatory cell infiltrates (arrows) and fibrotic thickening in addition to synovial membrane preservation (arrowhead). Panels A and B show peroxidase immunostaining of CD3+ (T lymphocytes), CD20+ (B lymphocytes) and CD68+ (macrophages) in the IA, IA/Suppl and Suppl groups. The synovial tissue of the control shows scarce CD3+ (E), CD20+ and CD68+ (A and B) cells. The immuno-expression of CD3+, CD20+ and CD68+ cells (arrows) in the synovial tissue of the IA/Suppl and Suppl groups is significantly decreased in relation to the IA group. Panels (A and B), H&E staining. Original magnification; 400×. Values are the means (±SEM) of 10 animals in each group. All values were completed in a few random, now coincident fields supplemented with daily doses for 30 days. Groups are compared using ANOVA. IA vs Suppl (p<0.01), IA vs IA/Suppl (p<0.01), Suppl vs IA (p<0.01).</p

Collagen V oral administration decreases inflammation and remodeling of synovial membrane in experimental arthritis

<div><p>Because collagen type V (Col V) can be exposed in tissue injury, we hypothesized that oral administration of this collagen species modulates the inflammation and remodeling of experimental synovitis, avoiding joint destruction, and that the modulation may differ according to the temporal administration. Arthritis (IA, n = 20) was induced in <i>Lewis</i> rats by intraarticular (ia) injection of 500 μg of methylated bovine serum albumin (mBSA) emulsified in complete Freund’s adjuvant (CFA) (10 μl) followed by an intraarticular booster of mBSA (50 μg) in saline (50 μl) administered at 7 and 14 days. The control group received saline (50 μl, ia). After the first intraarticular injection, ten IA animals were supplemented via gavage with Col V (500 μg/300 μl) daily for 30 days (IA/Suppl). The control group received saline (50 μL) and Col V supplement in the same way (Suppl). Col V oral administration in IA/Suppl led to 1) inhibited edema and severe inflammatory cell infiltration, 2) decreased collagen fiber content, 3) decreased collagen type I, 4) inhibited lymphocyte subpopulations and macrophages, 5) inhibited IL-1β, IL-10, IL-17 and TNF-α production and 6) increased expression of caspase-9 in the synovial tissue. In conclusion, Col V supplementation decreased synovial inflammation and the fibrotic response, possibly by increased the apoptosis of inflammatory cells.</p></div

Immuno-expression of IL-1β, IL-10, IL-17 and TNFα in the synovial sera from the IA, IA/Suppl and Suppl groups.

<p>Values are the means (±SEM) of 10 animals in each group. All values were completed in a few random, now coincident fields supplemented with daily doses for 30 days. Groups were compared using ANOVA. IA vs Suppl (p<0.01), IA vs IA/Suppl (p<0.01), Suppl vs IA (p<0.01).</p

Quantitative distribution of histological and immunohistochemical parameters in the synovia according to the groups studied.

<p>Quantitative distribution of histological and immunohistochemical parameters in the synovia according to the groups studied.</p

Serum anti-Col V antibodies level in the CT and IA groups 30 days after the first intraarticular injection of saline (CT) and methylated bovine serum albumin antigen (AI).

<p>Groups are compared using ANOVA. CT vs IA (p = 0.001).</p