A multiparametric assay to compare the cytotoxicity of soy milk with different storage media

Background/Aim: The aim of this study was to evaluate the cytotoxicity of soy milk compared with several other storage media [coconut water, Hank's Balanced Salt Solution (HBSS) and whole milk], assessed through a multiparametric analysis employing 3T3 cells. Materials and methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24 h, at 37 degrees C with 5% CO2, and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). Results from each test were compared by two-way analysis of variance (ANOVA). Results: Statistical analysis showed that whole milk, HBSS and soy milk were the most effective media in maintaining cell viability at all tested times (P < 0.05). The least amount of viable cells was observed when using coconut water. Conclusions: This study shows that the efficacy of soy milk in maintaining the viability of 3T3 fibroblasts is similar to that of HBSS and milk, as shown by three different cell viability tests.29431932

Long-term cytotoxic effects of contemporary root canal sealers

Objectives: The aim of the present study was to investigate the effects of root canal sealers on the cytotoxicity of 3T3 fibroblasts during a period of 5 weeks. Material and Methods: Fibroblasts (3T3, 1x10(5) cells per well) were incubated with elutes of fresh specimens from eight root canal sealers (AH Plus, Epiphany, Endomethasone N, EndoREZ, MTA Fillapex, Pulp Canal Sealer EWT, RoekoSeal and Sealapex) and with elutes of the same specimens for 5 succeeding weeks after immersing in simulated body fluid. The cytotoxicity of all root canal sealers was determined using the MU assay. Data were analyzed using ANOVA and Tukey's test. Results: RoekoSeal was the only sealer that did not show any cytotoxic effects (p<0.05). All the other tested sealers exhibited severe toxicity initially (week 0). MTA Fillapex remained moderately cytotoxic after the end of experimental period. Toxicity of the other tested sealers decreased gradually over time. The evaluated root canal sealers presented varying degrees of cytotoxicity, mainly in fresh mode. Conclusions: RoekoSeal had no cytotoxic effect both freshly mixed and in the other tested time points. MTA Fillapex was associated with significantly less cell viability when compared to the other tested root canal sealers.211434

Evaluation of Root Canal Configuration of Mandibular Molars in a Brazilian Population by Using Cone-beam Computed Tomography: An In Vivo Study

Introduction: The aim of this study was to analyze and characterize root canal morphology of mandibular molars of the Brazilian population by using cone-beam computed tomography (CBCT). Methods: Patients who required CBCT radiographic examinations as part of their routine examination, diagnosis, and treatment planning were enrolled in the study. A total of 460 healthy, untreated, fully developed mandibular first and second molars were included (234 first molars and 226 second molars). The following observations were recorded: (1) number of roots and their morphology, (2) number of canals per root, (3) C-shaped canals, and (4) primary variations in the morphology of the root canal systems. Results: First molars showed a higher prevalence of 2 canals in the mesial root and 1 in the distal root with 2 separate roots (74%). In the mandibular second molars, the presence of 2 separate roots with 2 canals in the mesial root and 1 canal in the distal root represented 54% of the total. In 32% of the cases, 2 separate roots with 1 canal each in the mesial and distal roots were presented. The incidence of C-shaped canals was 1.7% of first molars and 3.5% of second molars. Conclusions: A higher prevalence of 2 separate roots with 2 canals in the mesial root and 1 canal in the distal root was observed in mandibular first and second molars (74% and 54%, respectively). Also, a lower incidence of C-shaped canals and 3-rooted teeth was observed in a Brazilian population. CBCT is a clinically useful tool for endodontic diagnosis and treatment.39784985

Evaluation Of Root Canal Configuration Of Maxillary And Mandibular Anterior Teeth Using Cone Beam Computed Tomography: An In-vivo Study

Objective: Several studies demonstrate variability of root canal morphology of anterior teeth. This in-vivo study was designed to analyze and characterize root canal morphology of maxillary and mandibular anterior teeth using three-dimensional imaging. Method and Materials: Patients who required CBCT radiographic examinations as part of their routine examination, diagnosis, and treatment planning were enrolled on the study. A total of 1,200 healthy, untreated, fully developed maxillary and mandibular incisors and canines were included. The following observations were recorded: (i) number of roots and their morphology; (ii) number of canals per root; and (iii) primary variations in the morphology of the root canal systems. Results: Root canal morphology of maxillary teeth was consistent with a single canal extending from the pulp chamber to the apex in 98% of the cases. In contrast, in mandibular central and lateral incisors, the presence of a single canal represented 64.5% and 60.5% respectively. The second most common morphology was the presence of one canal leaving the pulp chamber, which divided into two within the root, and then merged to exit as one canal in 18% and 25.5% respectively. The presence of one canal leaving the pulp chamber and dividing short of the apex into two separate and distinct canals with separate apical foramina (variant 5) was recorded in 14.5% and 12% respectively. Conclusion: There was a low incidence of anatomical variation in maxillary anterior teeth. In contrast, mandibular anterior teeth presented with a high prevalence of two canals, especially central and lateral incisors. The reported data may help clinicians understand the variations in root canal morphology of anterior teeth in order to overcome problems associated with shaping and cleaning procedures.47192

Postoperative Pain after Foraminal Enlargement in Anterior Teeth with Necrosis and Apical Periodontitis: A Prospective and Randomized Clinical Trial

Introduction: The aim of this randomized, controlled, prospective clinical study was to determine if foraminal enlargement instrumentation during endodontic treatment is associated with more postoperative pain compared with standard nonenlargement instrumentation. Methods: Forty volunteers with a single root canal were diagnosed with asymptomatic necrosis with apical periodontitis and randomized into 2 experimental groups (ie, the control group and the foraminal enlargement group). Endodontic treatment was performed in a single visit, and volunteers were instructed to record pain intensity (ie, none, slight, moderate, and severe). Scores from 1 to 4 were attributed to each kind of pain after 12, 24, and 48 hours. The Kolmogorov-Smirnov and Student's t tests were used to determine significant differences at P .05, Student's t test). Postoperative pain showed no statistically significant difference between the groups at any observation period (P > .05). Also, no significant difference was observed in the mean number of analgesic tablets used between the groups (P > .05). Conclusions: The foraminal enlargement and nonenlargement techniques resulted in the same postoperative pain and necessity for analgesic medication. This may suggest that the use of foraminal enlargement should be performed for endodontic treatment previsibility without increasing postoperative pain. (J Endod 2013;39:173-176)39217317

Use of soymilk as a storage medium for avulsed teeth

Objective. Tooth avulsion is one of the most severe forms of dental trauma. In these cases, immediate reimplantation is ideal; however, it almost never happens. The purpose of this study was to evaluate the viability of cells stored in soymilk and compare with other several storage media. Materials and methods. The media tested were: long-shelf-life coconut water, long-shelf-life whole milk, long-shelf-life soymilk, Gatorade, egg white, and Hank's Balanced Salt Solution. Cells cultured in DMEM and distilled water served as positive and negative controls, respectively. Plates containing confluent 3T3 fibroblast were soaked in the various media for 2, 12 and 24 h. After incubation at 37 degrees C, viability of the cells was determined using the MTS assay. Data were analyzed by using one-way ANOVA and complemented by Tukey test with a significance level of 5%. Results. Statistical analysis showed that DMEM, whole milk, HBSS and soymilk were the most effective media for maintaining cell viability at all tested times (p < 0.05), followed by coconut water, egg white and Gatorade. The least amount of viable cells was observed in the distilled water group. Conclusions. The present study shows that the efficacy of soymilk in maintaining the viability of 3T3 fibroblasts is similar to that of HBSS and milk. Therefore, it can be concluded that soymilk could be a suitable alternative storage medium for avulsed teeth.7151101110