Medium- and high-cell-density production of Adeno-Associated virus serotype 6 and the mitigation of cell density effect via medium supplementation

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Descoloration of industrial dyes and simulated textile effluents dyes by turnip peroxidase

The removal of important textile dyes by turnip peroxidase (TNP) was evaluated. The textile effluents besides the residual dyes contain also chemical auxiliaries such as salts, dispersing and wetting agents. The effect of these was evaluated in the removal of the dyes reactive blue 21 and reactive blue 19 by TNP in synthetic effluents. A decrease of the efficency decolorization was observed. The action of the enzyme on colour removal of dye mixture was equivalent to the dyes alone. The chemical demand of oxygen in the effluent after enzymatic treatment had a significant increase in relation to the untreated effluent

Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC

AbstractDidanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV). An automated system using on-line solid extraction and High Performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosine in dog plasma. Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma. Extraction was carried out on C18 cartridges, with high extraction yield as stationary phase, whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer, acetonitrile (KH2PO4: acetonitrile: 96:4, v/v) and 0.5% (w/v) of heptane sulphonic acid. The pH was adjusted to 6.5 with triethylamine. All samples and standard solutions were chromatographed at 28°C. For an isocratic run, the flux was 1.0mL/min, detection was at 250nm and injected volume was 20μL. The method was selective and linear for concentrations between 50 and 5000ng/mL. Drug stability data ranged from 96% to 98%, and limit of quantification was 25ng/mL. Extraction yield was up to 95%. Drug stability in dog plasma was kept frozen at −20°C for one month after three freeze–thaw cycles, and for 24h after processing in the auto sampler. Assay was successfully applied to measure didanosine concentrations in plasma dogs

The AIVA fly-by-wireless UAV platform

The AIVA project concerns to an UAV aimed to perform aerial surveillance, forest fire detection and also to monitor high voltage cables for stress or failures. The global project involves the design and development of the required aerial platform, as well as the electronics, communications hardware and software, flight control, artificial vision and systems integration, in order to provide an autonomous takeoff, flight mission and landing manoeuvres. Relevant goals, regarding the design and development of the AIVA platform, initiated in September 2004, have already been achieved, and they will be described over next topics. [...

Pyrrolidine and Direct Covalent Linkage Recognition, and Multiple-Photo Response

The work was supported through projects UIDB/50006/2020 and UIDP/50006/2020, funded by FCT/MCTES, through national funds. S.L.H.R. (Refs. REQUIMTE/EEC2018/30) and A.M.G.S. thank FCT (Fundação para a Ciência e Tecnologia) for funding through program DL 57/2016—Norma transitória.To unveil and shape the molecular connectivity in (metallo)porphyrin–carbon nanotube hybrids are of main relevance for the multiple medicinal, photoelectronic, catalytic, and photocatalytic applications of these materials. Multi-walled carbon nanotubes (MWCNTs) were modified through 1,3-dipolar cycloaddition reactions with azomethine ylides generated in situ and carrying pentafluorophenyl groups, followed by immobilization of the β-amino-tetraphenylporphyrinate Zn(II). The functionalities were confirmed via XPS and FTIR, whereas Raman spectroscopy showed disruptions on the graphitic carbon nanotube surface upon both steps. The functionalization extension, measured via TGA mass loss and corroborated via XPS, was 0.2 mmol·g−1. Photophysical studies attest to the presence of the different porphyrin–carbon nanotube connectivity in the nanohybrid. Significantly different emission spectra and fluorescence anisotropy of 0.15–0.3 were observed upon variation of excitation wavelength. Vis-NIR absorption and flash photolysis experiments showed energy/charge transfer in the photoactivated nanohybrid. Moreover, evidence was found for direct reaction of amino groups with a carbon nanotube surface in the presence of molecular dipoles such as the zwitterionic sarcosine amino acid.publishersversionpublishe

A bluetooth-based wireless distributed data acquisition and control system

This paper presents an application independent embedded platform for a wireless distributed data acquisition and control system, and describes its application in the scenario of controlling the information processing and communications between sensors and actuators onboard of an autonomous flying robot, in a “fly-by-wireless” approach. The system, which was designed and implemented, comprises a set of nodes composed by microcontrollers, wireless communication modules based on Bluetooth technology, and sensing/actuation devices. This paper also presents several experimental results which denote encouraging performance characteristics for the developed system, not only in the context of the proposed application, but for other wireless applications as well

Distributed sensing and actuation over bluetooth for unmanned air vehicles

A short range wireless network platform, based on Bluetooth technology and on a Round Robin scheduling is presented. The objective is to build an application independent platform, to support a distributed sensing and actuation control system, which will be used in an Unmanned Aerial Vehicle (UAV). This platform provides the advantages of wireless communications while assuring low weight, small energy consumption and reliable communications

Obtention of plant peroxidase and its potential for the decolorization of the reactive dye Remazol Turquoise G 133%

Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum pH, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye Remazol Turquoise G 133%. The turnip peroxidase (TP) was utilized as an enzymatic source. This enzyme exhibited maximum activity at pH 7.0, and it was active in the temperature range of 30 to 50 °C, which favors its use in industrial processes. Acetone was the most efficient solvent to induce precipitation. The removal of Remazol Turquoise G 133% was 56.0% complete after 50 min, while 41.0% of the same dye was removed with the commercial horseradish peroxidase enzyme in 50 min. TP presents potential as a viable alternative in the decolorization of textile wastewaters

Descoloração de efluentes têxteis sintéticos por catálise enzimática

Peroxidases podem ser utilizadas em processos de descoloração no tratamento de efluentes têxteis. Há um interesse crescente por novas fontes desta enzima, e por processos de obtenção viáveis economicamente.1Neste trabalho o potencial de descoloração de efluentes têxteis sintéticos por peroxidase extraída de nabo (Brassica campestre ssp. rapifera) é avaliado. Para a obtenção da enzima de baixa pureza utiliza-se um processo de baixo custo, Os efluentes utilizados nos ensaios são efluentes sintéticos que simula as etapas de pré-tratamento e tingimento. Os corantes utilizados na preparação dos efluentes foram os corantes reativos Turqueza Remazol G 133% (CTR) e Remazol Brilliant Blue (RBBR) na concentração de 50mg/L. A remoção da cor de uma tricomia de corantes reativos contendo Remazol Brilliant Blue R, Remazol Vermelho Ultra (RVU) e Remazol Brilliant Orange 3R na concentração total de 50 mg/L, catalisada pela peroxidase do nabo também foi investigada. A descoloração obtida para os efluentes sintéticos contendo os corantes CTR e RBBR foi de 37 e 58%, respectivamente. Quanto à tricomia, a remoção da cor total foi de 40%, e a maior remoção observada foi de 69% para o corante RBBR na mistura, enquanto o corante RVU apresentou a menor remoção com apenas 18,7%.CAPES e CNP