Estará o Homem a medicar o Planeta?

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The effect of a joint clay-microorganism system to treat Ni and diethylketone solutions

The capacity of a combined system using a Streptococcus equisimilis biofilm supported in vermiculite to treat aqueous solutions polluted with diethylketone and nickel was accessed. In order to evaluate the interaction between the sorbent matrices and the two adsorbates several batch experiments were performed exposing 1) different amounts of vermiculite to Ni and diethylketone (Singular Sorbent Systems - SSS) or 2) a biofilm supported in different amounts of vermiculite to both pollutants (Binary Sorbent Systems - BSS). Fed batch pilot scale experiments were also conducted. For the SSS experiments, the removal of 3 g/L of DEK was complete for all the assays, whereas the removal of0.45 g/L of Ni ranged between 31 % and l00%. For the BSS experiments, the removal percentages of DEK and Ni decreased (77% to 97%, 23% to 97% respectively). Maximum removal percentages of 87.3% ofDEK and 57.6% of Ni were reached in the pilot scale experiments.Fundação para a Ciencia e Tecnologia, Ministerio da Ciencia e Tecnologia, Portugal and co-funding by FSE (programme QREN-POPH), by the "FCT Strategic Project PEst-OE/EQB/LA0023/2013" and by the "BioEnv- Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07- 0 I 24-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON.2-0 Novo Norte), QREN, FEDER

Bioremoval of hexavalent chromium by A. viscosus supported on Y and ZSM5 zeolites

Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/1096

Cleaner approach for atrazine removal using recycling biowaste/waste in permeable barriers

This work addresses the rehabilitation of water contaminated with atrazine, entrapping it in a permeable and sustainable barrier designed with waste materials (sepiolite) and with biomaterials (cork and pine bark). Atrazine adsorption was assessed by kinetics and equilibrium assays and desorption was tested with different extraction solvents. Adsorbed atrazine was 100% recovered from sepiolite using 20% acetonitrile solution, while 40% acetonitrile was needed to leach it from cork (98%) and pine bark (94%). Continuous fixed-bed experiments using those sorbents as PRB were performed to evaluate atrazine removal for up-scale applications. The modified dose-response model properly described the breakthrough data. The highest adsorption capacity was achieved by sepiolite (23.3 (±0.8) mg/g), followed by pine bark (14.8 (±0.6) mg/g) and cork (13.0 (±0.9) mg/g). Recyclability of sorbents was evaluated by adsorption-desorption cycles. After two regenerations, sepiolite achieved 81% of atrazine removal, followed by pine with 78% and cork with 54%. Sepiolite had the best performance in terms of adsorption capacity/stability. SEM and FTIR analyses confirmed no significant differences in material morphology and structure. This study demonstrates that recycling waste/biowaste is a sustainable option for wastewater treatment, with waste valorization and environmental protection.Portuguese Foundation for Science and Technology (FCT) under the scope of the research project PTDC/AAG-TEC/5269/2014, the strategic funding of UID/BIO/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte, Portugal. A. Lago thanks FCT for the concession of her PhD grant (SFRH/BD/132271/2017)info:eu-repo/semantics/publishedVersio

Biowaste valorization for emerging pollutant abatement in aqueous phase

The use of raw chemical- or thermal-modified pine bark (10 g/L) as a biosorbent to remove fluoxetine hydrochloride (FLX), carbamazepine (CRB) and atrazine (ATZ) from water at 5 mg/L each was explored in this work. The adsorption efficiency onto raw pine was as follows: FLX > ATZ > CRB. Pine oxidized with HNO3 revealed to be the best modified biosorbent in terms of overall sorbate entrapment capacity (1.95 mg/g). The performance of raw pine (as the most sustainable biosorbent) was assessed in a prepilot air-lift-type reactor as a rehabilitation system to treat contaminated water for upscale purposes.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the research project PTDC/AAG-TEC/5269/2014, the strategic funding of UID/BIO/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte, Portugal. A. Lago thanks FCT for her PhD grant (SFRH/BD/132271/2017).info:eu-repo/semantics/publishedVersio

Treatment of chromium(VI) solutions in a pilot-scale bioreactor through a biofilm of arthrobacter viscosus supported on GAC

The aim of this work is to evaluate the applicability of a biofilm to the removal of chromium in solution, at a pilot scale. The effect of the initial concentration of metal on the biosorption behavior of an Arthrobacter viscosus biofilm supported on granular activated carbon, in batch and column essays was also analyzed. Six isotherm equations have been tested in the present study. The best fit was obtained with the Freundlich model. It was observed that as the initial chromium concentration increases, the uptake increases too, but the removal percentage decreases, with values between 95.20% (C0 = 5 mg/l) and 38.28% (C0 = 1000 mg/l). The batch adsorption studies were used to develop a pilot bioreactor able to remove chromium from aqueous solutions. Data obtained in a pilot-scale reactor showed an average removal percentage of 99.9%, during the first 30 days, for the initial concentration of 10 mg/l and an average removal percentage of 72%, for the same period and for the initial concentration of 100 mg/l. Uptake values of 11.35 mg/g and 14.55 mg/g were obtained, respectively, for the initial concentration of 10 and 100 mg/l. The results obtained are very promising and encourage the utilization of this biofilm in environmental applications.FEDERFundação para a Ciência e a Tecnologia (FCT

Study of Ni (II) and diethylketone removal from aqueous solutions using a biofilm of Streptococcus equisimilis supported on vermiculite

The novelty of this work lies in the development of an environmental-friendly technology to remove diethylketone and Ni (II) from aqueous solutions using the interaction between bacteria and clays. Concentrated biomass of Streptococcus equisimilis and vermiculite were used for the removal of both pollutants No published work on the usage of this combination to remove heavy metals and organic solvents from aqueous solutions was acknowledged. The developed work is original and its impact on society and on environment is evident due to the toxicological effects of those substances on the health of living beings

Removal of organic compounds by a biofilm supported on GAC : modelling of batch and column data

The performance of a biofilm of Arthrobacter viscosus supported on granular activated carbon on the retention of organic compounds was evaluated. The presence of functional groups on the cell wall surface of the biomass that may interact with the organic compounds was confirmed by Fourier transform infrared spectroscopy, to assess the applicability of this system to the removal of those compounds. The batch assays showed that the removal percentage decreases with the increasing initial concentration. The removal of phenol ranged from 99.5 to 93.4%, the chlorophenol removal ranged from 99.3 to 61.6% and the o-cresol removal ranged from 98.7 to 73.5%, for initial concentrations between 100 and 1,700 mg/L. The batch data were described by Freundlich, Langmuir, Redlich–Peterson, Dubinin-Radushkevich, Sips and Toth model isotherms and the best fit for the retention of phenol and for the retention of o-cresol was obtained with the Sips model, while for chlorophenol, the best fit was obtained with the Freundlich model. The column tests showed that the retention performance followed the order: phenol > chlorophenol > o-cresol, and increased with the increasing initial organic compound concentration. Data from column runs were described by Adams–Bohart, Wolborska and Yoon and Nelson models with good fitting for all the models.POCTI/FEDER - POCTI/CTA/44449/2002Fundação para a Ciência e a Tecnologia (FCT

Current challenges for biological treatment of pharmaceutical-based contaminants with oxidoreductase enzymes: immobilization processes, real aqueous matrices and hybrid techniques

The worldwide access to pharmaceuticals and their continuous release into the environment have raised a serious global concern. Pharmaceuticals remain active even at low concentrations, therefore their occurrence in waterbodies may lead to successive deterioration of water quality with adverse impacts on the ecosystem and human health. To address this challenge, there is currently an evolving trend toward the search for effective methods to ensure efficient purification of both drinking water and wastewater. Biocatalytic transformation of pharmaceuticals using oxidoreductase enzymes, such as peroxidase and laccase, is a promising environmentally friendly solution for water treatment, where fungal species have been used as preferred producers due to their ligninolytic enzymatic systems. Enzyme-catalyzed degradation can transform micropollutants into more bioavailable or even innocuous products. Enzyme immobilization on a carrier generally increases its stability and catalytic performance, allowing its reuse, being a promising approach to ensure applicability to an industrial scale process. Moreover, coupling biocatalytic processes to other treatment technologies have been revealed to be an effective approach to achieve the complete removal of pharmaceuticals. This review updates the state-of-the-art of the application of oxidoreductases enzymes, namely laccase, to degrade pharmaceuticals from spiked water and real wastewater. Moreover, the advances concerning the techniques used for enzyme immobilization, the operation in bioreactors, the use of redox mediators, the application of hybrid techniques, as well as the discussion of transformation mechanisms and ending toxicity, are addressed.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnaical Systems, LA/P/0029/2020. Helena Sá thanks FCT for funding her PhD grant (UI/BD/151239/2021).info:eu-repo/semantics/publishedVersio

Recovery of biotreatment supports as catalysts in ciclohexene oxidation

EUROPACAT IX - Catalysis for a Sustainable WorldA biosorption system for the removal of hexavalent chromium from contaminated effiuents that allows the reutilization of the recovered supports as catalysts is proposed. The system consists of Arthrobacter viscosus bacterium supported on NaY zeolite. After the treatment of the effiuents, the metal-zeolite can be used in oxidation reactions. This work reports the optimized biotreatement conditions that lead to Cr-loaded in Na Y zeolite, which was tested as catalysts in the liquid-phase oxidation of cyclohexene. A comparison is made with a Cr-NaY zeolite prepared by the usual ion-exchange method.info:eu-repo/semantics/publishedVersio