6 research outputs found
Whole exome sequencing identifies new causative mutations in Tunisian families with non-syndromic deafness.
Identification of the causative mutations in patients affected by autosomal recessive non syndromic deafness (DFNB forms), is demanding due to genetic heterogeneity. After the exclusion of GJB2 mutations and other mutations previously reported in Tunisian deaf patients, we performed whole exome sequencing in patients affected with severe to profound deafness, from four unrelated consanguineous Tunisian families. Four biallelic non previously reported mutations were identified in three different genes: a nonsense mutation, c.208C>T (p.R70X), in LRTOMT, a missense mutation, c.5417T>C (p.L1806P), in MYO15A and two splice site mutations, c.7395+3G>A, and c.2260+2T>A, in MYO15A and TMC1 respectively. We thereby provide evidence that whole exome sequencing is a powerful, cost-effective screening tool to identify mutations causing recessive deafness in consanguineous families
Biallelic mutations identified in DFNB genes using a whole exome sequencing strategy.
<p>Biallelic mutations identified in DFNB genes using a whole exome sequencing strategy.</p
Pedigrees of the four Tunisian families analyzed using the whole exome sequencing strategy.
<p>Pedigrees of the four Tunisian families analyzed using the whole exome sequencing strategy.</p
Evolution of the number of variants during whole exome.
<p>Evolution of the number of variants during whole exome.</p
Sequences of the primers used to validate the mutations by Sanger sequencing.
<p>Sequences of the primers used to validate the mutations by Sanger sequencing.</p