Результаты изучения сортов сои Омского АНЦ в Костанайской области Республики Казахстан

The authors conducted research from 2012–2021 at LLP Agricultural Experimental Station “Zarechnoye” of the Kostanay region of Kazakhstan. The experiment included early maturing soybean varieties bred by the Omsk ANC, approved for cultivation in the Russian Federation: Dina, Sibiryachka and Zolotistaya, also zoned in the Republic of Kazakhstan. From May till September 2012, 2014, 2019, and 2022 dry weather conditions were observed (HTC 0.40 - 0.69); arid (HTC 0.70 - 0.99) - in 2015 - 2017, 2020; moderately humid (HTC 1.00) - in 2013 and 2018 A significant positive effect on the duration of the growing season was provided by an increase in the HTC coefficient for the period May–September (r = 0.566) and in July (r = 0.444). An increase in precipitation lengthened the growing season to 113 days, and an increase in air temperature accelerated the growing season to 83 days (r = -0.353 and r = -0.440, respectively). The most stable was the plant height, which varied depending on the conditions of the year and variety from 55 to 76 cm - the coefficient of variation was about 10%. Significant varietal differences in average long-term values were revealed only by the number of pods per plant - the Sibiryachka cultivar had an advantage over the Dina and Zolotistaya cultivars, having formed an average of 36 pods per plant over ten years. The level of seed yield was significantly affected by the amount of precipitation (r = 0.608) and air temperature (r = 0.632) in June, as well as the number of productive nodes (r = 0.365) per plant. The increase in the share of protein was positively influenced by the rise in the amount of precipitation in June and in general for May–September: r = 0.415 and r = 0.581, respectively, as well as by the HTC for these periods: r = 0.362 and r = 0.561. A significant positive correlation was found between the protein content and the weight of 1000 seeds (r = 0.615) and a not high but significant positive correlation between the weight of 1000 seeds and the amount of precipitation in August (r = 0.375). The maximum seed yield and protein content in the experiment were noted in the Dina variety in 2016 - 2.78 t/ha and 40.6%, respectively. Variety Zolotistaya has the best resistance to stress in these indicators. Types differed in plasticity: Dina - in terms of yield (bi = 1.11) and Sibiryachka - in terms of weight of 1000 seeds (bi = 1.18). All varieties of the selection of the Omsk ANC included in the experiment can be used in breeding work in the conditions of the Kostanay region as sources of early maturity and yield - they need from 93 to 110 days to form more than 2.4 t/ha of seeds.Исследования проведены в 2012–2021 гг. в ТОО СХОС «Заречное» Костанайской области Республики Казахстан. В эксперимент включены скороспелые сорта сои селекции Омского АНЦ, допущенные для возделывания в РФ: Дина, Сибирячка и Золотистая, районированная и в РК. В мае–сентябре в 2012, 2014, 2019, 2022 гг. наблюдались сухие погодные условия (ГТК 0,40 – 0,69); засушливые (ГТК 0,70 – 0,99) – в 2015 – 2017, 2020 гг.; умеренно влажные (ГТК 1,00) – в 2013 и 2018 гг. Достоверное положительное влияние на продолжительность вегетационного периода оказывало повышение коэффициента ГТК за период май–сентябрь (r = 0,566) и в июле (r = 0,444). Увеличение суммы осадков удлиняло период вегетации до 113 сут, повышение температуры воздуха ускоряло вегетацию до 83 сут (r = –0,353 и r = –0,440 соответственно). Наиболее стабильной была высота растений, которая изменялась в зависимости от условий года и сорта от 55 до 76 см – коэффициент вариации около 10%. Достоверные сортовые различия средних многолетних значений выявлены лишь по числу бобов на растении – сорт Сибирячка имел преимущество перед сортами Дина и Золотистая, сформировав в среднем за 10 лет 36 бобов на растении. На уровень урожайности семян достоверное влияние оказывали сумма осадков (r = 0,608) и температура воздуха (r = -0,632) в июне, а также число продуктивных узлов (r = 0,365) на растении. На повышение доли белка положительно влияло увеличение суммы осадков в июне и в целом за май–сентябрь: r = 0,415 и r = 0,581 соответственно, а также ГТК за эти периоды: r = 0,362 и r = 0,561. Выявлена существенная положительная корреляционная связь содержания белка с массой 1000 семян (r = 0,615) и не высокая, но достоверная положительная корреляция массы 1000 семян с суммой осадков в августе (r = 0,375). Максимальные в опыте урожайность семян и содержание в них белка отмечены у сорта Дина в 2016 г. – 2,78 т/га и 40,6% соответственно. У сорта Золотистая лучшая стрессоустойчивость по этим показателям. Пластичностью отличались сорта: Дина – по урожайности (bi  = 1,11) и Сибирячка – по массе 1000 семян (bi  = 1,18). Все сорта селекции Омского АНЦ, включенные в эксперимент, могут быть использованы в селекционной работе в условиях Костанайской области в качестве источников скороспелости и урожайности – для формирования более 2,4 т/га семян им требуется от 93 до 110 сут

Interaction between Hsp60 and Bax in normal human myocardium and in myocardium affected by dilated cardiomyopathy

The main functional compartments of molecular chaperone Hsp60 are mitochondria and cytoplasm. Up to 30 % of Hsp60 are located in cytoplasm of cardiomyocytes. The interaction between molecular chaperone Hsp60 and proapoptotic Bax protein in the cytoplasmic fraction from normal human heart tissue has been revealed by co-immunoprecipitation in contrast to myocardium affected by dilated cardiomyopathy, where this interaction has not been observe

Hsp60 and p70S6K form a complex in human cardiomyocytes

Molecular chaperon Hsp60 and protein kinase p70S6K play an important functional role in the regulation of cardiomyocytes vital function or apoptosis. Aim. To study a possibility of in vivo complex formation between Hsp60 and p70S6K in cardiomyocytes. Methods. Co-immunoprecipitation, Western-blot analysis. Results. We have identified in vivo interaction between molecular chaperone Hsp60 and two isoforms of proteinkinase p70S6K in human myocardium, normal and affected by cardiomyopathy. Conclusions. The results obtained suggest a possible participation of molecular chaperon Hsp60 in regulation of p70S6K activity in stressinduced apoptotic signaling pathway in cardiomyocytes

Identification of autoantibodies to tyrosil-tRNA synthetase in heart disfunctions

Aim. To investigate the levels of specific autoantibodies against tyrosyl-tRNA synthetase and its individual modules in the blood serum of people with heart failure caused by dilated cardiomyopathy, myocarditis and ischemic heart disease compared with healthy donors. Methods. Recombinant proteins were obtained using bacterial strains transformed with appropriate plasmid vectors and were purified by chromatography on Ni-NTA-agarose. The levels of specific autoantibodies were investigated by ELISA. Results. The increased levels of autoantibodies specific to tyrosyl-tRNA synthetase, its N-terminal catalytic module and non-catalytic C-module, were found in the blood serum of patients, compared with healthy donors. Conclusions. The results obtained demonstrate the possible role of tyrosyl-tRNA synthetase in adaptive changes of the myocardium in response to stress factors

Expression and subcellular localization of p70S6 kinase under heart failure

The PI3K/PDK/Akt/mTOR/p70S6K signaling pathway is primary associated with the activation of insulin receptors and is important for cardiomyocytes survival. p70S6K is a key regulator of the speed and efficiency of protein biosynthesis within the cell. Recently the pro-apoptotic protein BAD has been identified as a new target for р70S6K1. BAD is inactivated in normal cardiomyocytes by р70S6K1 phosphorylation which prevents the cardiomyocytes apoptosis. Aim. To study possible changes in р70S6K1 expression and/or cellular localization at heart failure progression – in DCM- affected human myocardia and murine hearts with experimental DCM-like pathology. Methods. Western-blot analysis and immunohystochemistry. Results. The substantial decrease in р70S6K1 level was observed at the final stage of pathology progression and in the dynamics of DCM pathogenesis as well. For the first time relocalization of the protein to the connective tissue was shown according to the Western-blot results. Conclusions. The data obtained allow us to understand a possible role of р70S6K1 in the regulation of stress-induced apoptotic signaling in cardiomyocytes

Multisubunit complex eEF1H in human glial tumors: from mRNA to protein

Aim. To investigate protein level of all subunits of the eukaryotic elongation translation factor eEF1H (eEF1A, eEF1Bα, eEF1Bβand eEF1Bγ) in glial tumors of human brain in comparison with normal brain. Methods. The eEF1H components content has been investigated in human glioblastoma clinical samples by Western blot analysis. Results. To determine the eEF1Bα, eEF1Bβ and eEF1Bγ content, the polyclonal antibodies against all eEF1H subunits were obtained. The tendency of the eEF1Bγ protein level to increase in glioblastomas was observed. There were no significant differences in the eEF1A, eEF1Bα and eEF1Bβ protein contents. Conclusions. In the previous report we analysed the expression of all eEF1H subunits in human glial brain tumor on the mRNA level. This study showed that eEF1Bγ was overexpressed while no significant changes in other eEF1H subunits were observed. It suggests a possible function of eEFBg which is cancer-related and is not connected with the functioning of eEF1H complex in translation

Potentiation effect of the AMPK activator A-769662 on cardiac myocytes metabolism and survival

Abstract 286 van Poster session 2 Frontiers in CardioVascular Biology, London 30th March – 1st April 2012 Second Congress of the ESC Council on Basic Cardiovascular Science