59 research outputs found
Factors influencing the effectiveness of non-pathogenic Fusarium solani strain Fs5 in the suppression of root-knot nematode in tomato
Four experiments were carried out under greenhouse conditions to evaluate the effectiveness of Fusarium
solani strain Fs5 against the root-knot nematode Meloidogyne javanica. The effect of population densities of M.
javanica, various application rates of F. solani, moisture regimes and levels of benzaldehyde, a volatile compound of
plant origin affecting the plant-nematode-fungus interaction, were also studied. F. solani parasitized eggs and females
of M. javanica and thereby reduced root-knot severity in tomato. Although the fungus was frequently isolated
from root tissues, it did not produce phytotoxic symptoms; instead, there was enhanced plant growth. At higher
nematode densities, inner root colonization by the fungus increased. The rates of fungal infection on M. javanica eggs
and females also increased with increasing nematode densities and fungal inoculum levels. Nematode invasion and
subsequent root-knot increased with increasing soil moisture, in both F. solani-treated and untreated plants. However,
root-knot development was lower at all moisture regimes when F. solani was applied to the soil. Root colonization
by F. solani and parasitism on female nematodes was highest at 50% moisture holding capacity (MHC) whereas
egg parasitism by the fungus was greatest at 75% MHC. With increasing concentration of benzaldehyde in soil,
nematode penetration and subsequent root-knot infection were progressively reduced. Root colonization by F. solani
was greatest in soil treated with benzaldehyde at 2 ”g g-1 of soil in the presence of M. javanica. Increasing benzaldehyde
concentrations resulted in increased parasitism of M. javanica females by F. solani but in lower parasitism of the eggs.
Treatments with F. solani led to better plant growth when they were combined with benzaldehyde at 2 ”g g-1 of soil
Phenol-mediated suppression of soil-borne root-infecting fungi in mungbean
Under field conditions, there is a variety of phenolic acids as well as other toxic and non-toxic organic
compounds that interact with plant seeds and roots; but in laboratory bioassays, with few exceptions, only single
phenolic acids are usually tested. In this study, the effect of various concentrations of two phenolics (caffeic acid
and p-hydroxybenzoic acid) on the wilt-inducing fungus Fusarium solani and the damping-off fungus Rhizoctonia
solani was tested in pot experiments. The effect of these phenolics on the biocontrol efficacy of Pseudomonas
aeruginosa, a plant growth-promoting rhizobacterium, was also evaluated. Caffeic acid and p-hydroxybenzoic acid
significantly suppressed F. solani and R. solani infection in mungbean. However, high concentrations of the phenolic
acids interfered with plant growth. P. aeruginosa in the rhizosphere declined in the presence of caffeic acid
and p-hydroxybenzoic acid
Avicennia marina (mangrove) soil amendment changes the fungal community in the rhizosphere and root tissue of mungbean and contributes to control of root-knot nematodes
The effect of soil amendment with Avicennia marina (mangrove) on mungbean growth and mungbean
infestation with Meloidogyne javanica was determined in greenhouse pot experiments. Galling and final nematode
population densities were reduced by all soil amendments with mangrove. To better understand whether nematode
suppression by A. marina was caused directly by the release of nematicidal factor(s) into the soil, or was due indirectly
to changes in the fungal community, the diversity of the rhizosphere populations of culturable fungi was assessed
before organic amendment (day 0), after decomposition but before seed sowing (day 15) and at harvest (day
73). Thirteen out of 20 fungal species were isolated from both A. marina-amended and unamended soils, the most
frequent genera being Alternaria, Aspergillus, Fusarium, Penicillium, Trichoderma, Mucor, Myrothecium and
Rhizoctonia. The other seven were found only in amended soils. At different times in the course of the experiment
amended and unamended soils differed significantly in the fungi isolated from the rhizosphere and/or in the concentrations
of A. marina. Trichoderma viride was isolated only from surface-sterilized mungbean roots grown in amended
soils, whereas Chaetomium sp. was isolated only from unamended soils
Non-Newtonian effects in the peristaltic flow of a Maxwell fluid
We analyzed the effect of viscoelasticity on the dynamics of fluids in porous
media by studying the flow of a Maxwell fluid in a circular tube, in which the
flow is induced by a wave traveling on the tube wall. The present study
investigates novelties brought about into the classic peristaltic mechanism by
inclusion of non-Newtonian effects that are important, for example, for
hydrocarbons. This problem has numerous applications in various branches of
science, including stimulation of fluid flow in porous media under the effect
of elastic waves. We have found that in the extreme non-Newtonian regime there
is a possibility of a fluid flow in the direction {\it opposite} to the
propagation of the wave traveling on the tube wall.Comment: to Appear in Phys. Rev. E., 01 September 2001 issu
Nematicidal and allelopathic responses of Lantana camara root extract
The impact of root leachates of Lantana camara L., a tropical weed, against Meloidogyne javanica, the
root-knot nematode, was tested under laboratory and pot conditions. Concentrated and diluted root leachate caused
substantial mortality of M. javanica juveniles. Significant suppression of the nematode was achieved when soil was
treated with a full-strength concentration of the leachate. Whilst this high concentration retarded plant height and
shoot fresh weight, more diluted concentrations actually enhanced plant growth. To establish whether this inhibition
of plant growth from the leachate was the result of depleted nitrogen levels in the soil due to the leachate, soil treated
with such leachates was given urea as an additional nitrogen source. Urea not only enhanced nematode suppression
activity of the root leachates but also increased seedling emergence and growth of mungbean. Application of the L.
camara root leachates in combination with Pseudomonas aeruginosa, a plant growth-promoting rhizobacterium,
significantly reduced nematode population densities in roots and subsequent root-knot infection, and enhanced plant
growth. While a high concentration of root leachate slightly reduced P. aeruginosa colonization in the rhizosphere
and inner root tissues, the nematicidal efficacy of the bacterium was unaffected. The root leachate of L. camara was
found to contain phenolic compounds, including p-hydroxybenzoic acid, vanillic acid, caffeic acid, ferulic acid and a
quercetin glycoside, 7-glucoside. It also contained weak enzymic hydrogen cyanide
Estudo Comparativo da Biologia de Meloidogyne enterolobii (= M. mayaguensis) e Meloidogyne javanica em tomateiros com gene Mi
Development of modified medium for the enhancement in antifungal activity of P. steckii (MF1 mangrove fungi) against verticillium wilt pathogenic fungi of rose
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Gaia Early Data Release 3: The celestial reference frame (Gaia-CRF3)
Context. Gaia-CRF3 is the celestial reference frame for positions and proper motions in the third release of data from the Gaia mission, Gaia DR3 (and for the early third release, Gaia EDR3, which contains identical astrometric results). The reference frame is defined by the positions and proper motions at epoch 2016.0 for a specific set of extragalactic sources in the (E)DR3 catalogue. Aims. We describe the construction of Gaia-CRF3 and its properties in terms of the distributions in magnitude, colour, and astrometric quality. Methods. Compact extragalactic sources in Gaia DR3 were identified by positional cross-matching with 17 external catalogues of quasi-stellar objects (QSO) and active galactic nuclei (AGN), followed by astrometric filtering designed to remove stellar contaminants. Selecting a clean sample was favoured over including a higher number of extragalactic sources. For the final sample, the random and systematic errors in the proper motions are analysed, as well as the radio-optical offsets in position for sources in the third realisation of the International Celestial Reference Frame (ICRF3). Results. Gaia-CRF3 comprises about 1.6 million QSO-like sources, of which 1.2 million have five-parameter astrometric solutions in Gaia DR3 and 0.4 million have six-parameter solutions. The sources span the magnitude range G = 13-21 with a peak density at 20.6 mag, at which the typical positional uncertainty is about 1 mas. The proper motions show systematic errors on the level of 12 ÎŒas yr-1 on angular scales greater than 15 deg. For the 3142 optical counterparts of ICRF3 sources in the S/X frequency bands, the median offset from the radio positions is about 0.5 mas, but it exceeds 4 mas in either coordinate for 127 sources. We outline the future of Gaia-CRF in the next Gaia data releases. Appendices give further details on the external catalogues used, how to extract information about the Gaia-CRF3 sources, potential (Galactic) confusion sources, and the estimation of the spin and orientation of an astrometric solution
The Cholecystectomy As A Day Case (CAAD) Score: A Validated Score of Preoperative Predictors of Successful Day-Case Cholecystectomy Using the CholeS Data Set
Background
Day-case surgery is associated with significant patient and cost benefits. However, only 43% of cholecystectomy patients are discharged home the same day. One hypothesis is day-case cholecystectomy rates, defined as patients discharged the same day as their operation, may be improved by better assessment of patients using standard preoperative variables.
Methods
Data were extracted from a prospectively collected data set of cholecystectomy patients from 166 UK and Irish hospitals (CholeS). Cholecystectomies performed as elective procedures were divided into main (75%) and validation (25%) data sets. Preoperative predictors were identified, and a risk score of failed day case was devised using multivariate logistic regression. Receiver operating curve analysis was used to validate the score in the validation data set.
Results
Of the 7426 elective cholecystectomies performed, 49% of these were discharged home the same day. Same-day discharge following cholecystectomy was less likely with older patients (OR 0.18, 95% CI 0.15â0.23), higher ASA scores (OR 0.19, 95% CI 0.15â0.23), complicated cholelithiasis (OR 0.38, 95% CI 0.31 to 0.48), male gender (OR 0.66, 95% CI 0.58â0.74), previous acute gallstone-related admissions (OR 0.54, 95% CI 0.48â0.60) and preoperative endoscopic intervention (OR 0.40, 95% CI 0.34â0.47). The CAAD score was developed using these variables. When applied to the validation subgroup, a CAAD score of â€5 was associated with 80.8% successful day-case cholecystectomy compared with 19.2% associated with a CAAD score >5 (pâ<â0.001).
Conclusions
The CAAD score which utilises data readily available from clinic letters and electronic sources can predict same-day discharges following cholecystectomy
Multiorgan MRI findings after hospitalisation with COVID-19 in the UK (C-MORE): a prospective, multicentre, observational cohort study
Introduction:
The multiorgan impact of moderate to severe coronavirus infections in the post-acute phase is still poorly understood. We aimed to evaluate the excess burden of multiorgan abnormalities after hospitalisation with COVID-19, evaluate their determinants, and explore associations with patient-related outcome measures.
Methods:
In a prospective, UK-wide, multicentre MRI follow-up study (C-MORE), adults (aged â„18 years) discharged from hospital following COVID-19 who were included in Tier 2 of the Post-hospitalisation COVID-19 study (PHOSP-COVID) and contemporary controls with no evidence of previous COVID-19 (SARS-CoV-2 nucleocapsid antibody negative) underwent multiorgan MRI (lungs, heart, brain, liver, and kidneys) with quantitative and qualitative assessment of images and clinical adjudication when relevant. Individuals with end-stage renal failure or contraindications to MRI were excluded. Participants also underwent detailed recording of symptoms, and physiological and biochemical tests. The primary outcome was the excess burden of multiorgan abnormalities (two or more organs) relative to controls, with further adjustments for potential confounders. The C-MORE study is ongoing and is registered with ClinicalTrials.gov, NCT04510025.
Findings:
Of 2710 participants in Tier 2 of PHOSP-COVID, 531 were recruited across 13 UK-wide C-MORE sites. After exclusions, 259 C-MORE patients (mean age 57 years [SD 12]; 158 [61%] male and 101 [39%] female) who were discharged from hospital with PCR-confirmed or clinically diagnosed COVID-19 between March 1, 2020, and Nov 1, 2021, and 52 non-COVID-19 controls from the community (mean age 49 years [SD 14]; 30 [58%] male and 22 [42%] female) were included in the analysis. Patients were assessed at a median of 5·0 months (IQR 4·2â6·3) after hospital discharge. Compared with non-COVID-19 controls, patients were older, living with more obesity, and had more comorbidities. Multiorgan abnormalities on MRI were more frequent in patients than in controls (157 [61%] of 259 vs 14 [27%] of 52; p5mg/L, OR 3·55 [1·23â11·88]; padjusted=0·025) than those without multiorgan abnormalities. Presence of lung MRI abnormalities was associated with a two-fold higher risk of chest tightness, and multiorgan MRI abnormalities were associated with severe and very severe persistent physical and mental health impairment (PHOSP-COVID symptom clusters) after hospitalisation.
Interpretation:
After hospitalisation for COVID-19, people are at risk of multiorgan abnormalities in the medium term. Our findings emphasise the need for proactive multidisciplinary care pathways, with the potential for imaging to guide surveillance frequency and therapeutic stratification.
Funding:
UK Research and Innovation and National Institute for Health Research
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