Effects of preservation time and temperature on mitochondrial distribution and GSH level in mouse oocytes.

<p>(A) Mitochondrial distribution was evaluated by fluorescence microscopy. Three localization patterns were observed: perinuclear, homogenous and clustered. (B) The percentage of abnormal mitochondrial distribution in GV oocytes in the control group (n = 279) and preservation group at different temperatures. * indicate statistically significant differences (P<0.05). (C) The GSH concentrations in GV oocytes (n = 30) in different groups.</p

Effects of different preservation periods and temperatures on oocyte spindle configuration after IVM.

<p>Oocytes cultured in M2 medium for 8h (MI) and 12h (MII), spindle was stained with FITC-α-tubulin and DNA was stained with PI. (A, B,C) the normal spindle in mouse MI and MII oocyte in control group and experimental group at different preservation temperatures.</p

Effects of different carcass preservation temperatures on oocyte meiotic process.

<p>(A) The number of GV oocytes collected from mouse carcass after preserved for 4 hours at different temperatures. (B) The percentage of GVBD oocytes and PBE in PMI 4 h group at different preservation temperatures. Each bar represents mean ±SEM (n = 3). * P<0.05.</p

Hierarchical clustering of lncRNA and mRNA by Z-score.

<p>Based on the expression levels of (A) lncRNAs or (B) mRNAs, the six samples were classified into two groups (sperm from diabetic or control mice). The dendrogram shows the relationships among the expression levels of samples. Red indicates high relative expression, and green indicates low relative expression.</p

Gene ontology enrichment for lncRNA targets in the category of biological processes from sperm samples.

<p>Gene ontology analysis of lncRNA-target genes according to (A) biological process, (B) cell component, and (C) molecular function.</p

Microarray and quantitative PCR.

<p>Microarray and quantitative PCR for lncRNAs (Uc007gwn.1, NR_015547, ENSMUST00000134455) and mRNAs (Spa17, Mbd311, prm3). The quantitative PCR results were consistent with the microarray data.</p

The color-coded CNC network (1700009J07Rik, Spats21, and Gm16180).

<p>Blue (RGB: 0, 205, 205) are round nodes that represent coding genes, and pink (RGB: 255, 204, 204) are square nodes representing noncoding genes. The red solid line between the two nodes represents a positive correlation, and the blue dashed line represents a negative correlation. The difference between nodes associated with upregulated genes is marked with red, while the difference between nodes associated with downregulated genes is marked with green.</p

Plots of lncRNA and mRNA.

<p>Box plot of (A) lncRNA and (D) mRNA. Scatter plot of (B) lncRNA and (E) mRNA. Volcano plot of (C) lncRNA and (F) mRNA. Plots were constructed using fold-change and p values, enabling visualization of the relationship between fold change (magnitude of change) and statistical significance (which takes both magnitude of change and variability into consideration). The vertical lines correspond to a 2-fold change in expression (up or down), and the horizontal line represents <i>p</i> = 0.05. The red point in the plot represents the differentially expressed genes with statistical significance.</p