18 research outputs found
ΠΡΠΎΠ΅ΠΊΡ ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΏΠΎΠ΄ΡΡΠ°Π½ΡΠΈΠΈ 35/6 ΠΊΠ ΠΡΠ°Π²ΠΎΠ±Π΅ΡΠ΅ΠΆΠ½Π°Ρ
ΠΠΈΠΏΠ»ΠΎΠΌΠ½Π°Ρ ΡΠ°Π±ΠΎΡΠ° 74 Ρ., 1 ΡΠΈΡΡΠ½ΠΊΠΎΠ², 10 ΡΠ°Π±Π»ΠΈΡ, 22 ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠ°.
ΠΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠ΄ΡΡΠ°Π½ΡΠΈΡ Β«ΠΡΠ°Π²ΠΎΠ±Π΅ΡΠ΅ΠΆΠ½Π°ΡΒ».
Π ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡΡ ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ ΠΏΠΎΠ΄ΡΡΠ°Π½ΡΠΈΠΈ, Π½Π°ΠΏΡΡΠΆΠ΅Π½ΠΈΠ΅ΠΌ 35/6 ΠΊΠ. ΠΡΡΡΠ΅ΡΡΠ²Π»ΡΠ΅ΡΡΡ ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠ° ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΎΡΠ° Π’ΠΠΠ‘ 16000/35/6, Π΄Π»Ρ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΠΌΠΎΡΠ½ΠΎΡΡΠΈ. ΠΠ»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π·Π°ΡΡΠ°Ρ Π½Π° ΡΠ΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ Π±ΡΠ» ΠΏΡΠΎΠΈΠ·Π²Π΅Π΄Π΅Π½ ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ°ΡΡΠ΅Ρ. Π’Π°ΠΊ ΠΆΠ΅ Π±ΡΠ» ΠΏΡΠΎΠΈΠ·Π²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΈ ΡΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΡΡΠΈ.
ΠΡΠΏΡΡΠΊΠ½Π°Ρ ΠΊΠ²Π°Π»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌ Mathcad 15 ΠΈ ΡΠ΅ΠΊΡΡΠΎΠ²ΠΎΠΌ ΡΠ΅Π΄Π°ΠΊΡΠΎΡΠ΅ MS Word 2007 ΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° Π½Π° ΠΊΠΎΠΌΠΏΠ°ΠΊΡ - Π΄ΠΈΡΠΊΠ΅ (Π² ΠΊΠΎΠ½Π²Π΅ΡΡΠ΅ Π½Π° ΠΎΠ±ΠΎΡΠΎΡΠ΅ ΠΎΠ±Π»ΠΎΠΆΠΊΠΈ).Thesis 74 p., 1 pictures, 10 tables, 22 source.
The object of this study is to substation "Right-Bank".
The work is carried out the reconstruction of substations 35/6 kV voltage. Implemented by installing the transformer TDNS 16000/35/6, ββto increase capacity. To determine the costs for the reconstruction of economic calculation was made. The same was made the analysis of industrial and environmental safety.
Final qualifying work done with Mathcad 15 programs and MS Word 2007 text editor, and is represented on the CD - ROM (in an envelope on the back cover)
Riccardin D Exerts Its Antitumor Activity by Inducing DNA Damage in PC-3 Prostate Cancer Cells <i>In Vitro</i> and <i>In Vivo</i>
<div><p>We recently reported that Riccardin D (RD) was able to induce apoptosis by targeting Topo II. Here, we found that RD induced cell cycle arrest in G2/M phase in PC-3 cells, and caused remarkable DNA damage as evidenced by induction of Ξ³H2AX foci, micronuclei, and DNA fragmentation in Comet assay. Time kinetic and dose-dependent studies showed that ATM/Chk2 and ATR/Chk1 signaling pathways were sequentially activated in response to RD. Blockage of ATM/ATR signaling led to the attenuation of RD-induced Ξ³H2AX, and to the partial recovery of cell proliferation. Furthermore, RD exposure resulted in the inactivation of BRCA1, suppression of HR and NHEJ repair activity, and downregulation of the expressions and DNA-end binding activities of Ku70/86. Consistent with the observations, microarray data displayed that RD triggered the changes in genes responsible for cell proliferation, cell cycle, DNA damage and repair, and apoptosis. Administration of RD to xenograft mice reduced tumor growth, and coordinately caused alterations in the expression of genes involved in DNA damage and repair, along with cell apoptosis. Thus, this finding identified a novel mechanism by which RD affects DNA repair and acts as a DNA damage agent in prostate cancer.</p> </div
RD inhibits tumor growth in xenograft mice.
<p><i>A</i>-<i>C</i>, Effect of RD on tumor growth and body weight of nude mice. <i>D</i>, western blot analysis of DNA damage and repair, and apoptosis proteins from tumor tissues. <i>E</i>, immunofluorescence staining of p-BRCA1 and Ξ³H2AX in RD-treated and placebo tissues. Nuclei were stained with DAPI. Scale bars, 100 Β΅m. <i>F</i>, <i>a</i>, H&E staining of tissues from RD- and placebo-treated nude mice. <i>b</i>, Tissues were fixed and stained by TUNEL followed by DAB staining. Nuclei were counterstained with methyl green. Brown stain around green nuclei indicates apoptotic cells.</p
Effect of RD on DNA damage response signalings.
<p><i>A</i>, Changes of DNA damage proteins in RD-treated cells were analyzed by western blotting. <i>B</i>, After treatment with chemicals for 4h or 12h, protein levels of DNA damage proteins were detected by western blotting. <i>C</i>, Immunoο¬uorescence staining of Ξ³H2AX foci and p-BRCA1 foci in PC-3 cells. <i>D</i>, <i>a</i>, Neutral comet assay of PC-3 cells treated with RD for 4h and 12h. <i>b</i>, Comet length was analyzed by box and whisker plot method (100 cells per sample). <i>E</i>, Associations of Ξ³H2AX, PP2AC, and PPP4C were determined by coimmunoprecipitation using anti-Ξ³H2AX, anti-PP2AC, anti-PPP4C, or normal IgG. <i>F</i>, PC-3 cells were pretreated with 10 mmol/L caffeine for 1h, and exposed to RD for 4h and 12h, <i>a</i>, cell viability measured by MTT assay; bars, SD. *, <sup>#</sup>, P < 0.05, significant difference from control. <i>b</i>, changes of Ξ³H2AX were detected by western blotting.</p