15 research outputs found
Microbiomic profiles of bile in patients with benign and malignant pancreaticobiliary disease.
BackgroundThe prognostic and pathophysiologic significance of the biliary microbiota in pancreaticobiliary malignancies is little understood. Our goal was to find malignancy-related microbiomic fingerprints in bile samples taken from patients with benign and malignant pancreaticobiliary diseases.MethodsBile specimens were collected from consenting patients during routine endoscopic retrograde cholangiopancreatography. We used PowerViral RNA/DNA Isolation kit to extract DNA from bile specimens. The Illumina 16S Metagenomic Sequencing Library Preparation guide was used to amplify the bacterial 16S rRNA gene and create libraries. QIIME (Quantitative Insights Into Microbial Ecology), Bioconductor phyloseq, microbiomeSeq, and mixMC packages were used for post-sequencing analysis.ResultsOf 46 enrolled patients, 32 patients had pancreatic cancers, 6 had cholangiocarcinoma and 1 had gallbladder cancer. Rest of the patients had benign diseases including gallstones, and acute and chronic pancreatitis. We used multivariate approach in mixMC to classify Operational Taxonomic Units (OTUs). Doing this, we found a predominance of genera Dickeya (p = 0.00008), [Eubacterium] hallii group (p = 0.0004), Bacteroides (p = 0.0006), Faecalibacterium (p = 0.006), Escherichia-Shigella (p = 0.008), and Ruminococcus 1 (p = 0.008) in bile samples from pancreaticobiliary cancers as compared to benign diseases. Additionally, bile samples from patients with pancreatic cancer exhibited a predominance of genus Rothia (p = 0.008) as compared to those with cholangiocarcinoma, whereas bile samples from patients with cholangiocarcinoma exhibited a predominance of genera Akkermansia (p = 0.031) and Achromobacter (p = 0.031) as compared to those with pancreatic cancers.ConclusionsBoth benign and malignant pancreaticobiliary diseases have distinct microbiomic fingerprints. The relative abundance of OTUs in bile samples varies between patients with benign and malignant pancreaticobiliary diseases, as well as between cholangiocarcinoma and pancreatic cancer. Our data suggest that either these OTUs play a role in carcinogenesis or that benign disease-specific microenvironmental changes differ from cancer-specific microenvironmental changes, resulting to a clear separation of OTU clusters. We need more research to confirm and expand on our findings
Clinical Outcomes of Isolated Distal Deep Vein Thrombosis Associated with Cancer: The Cleveland Clinic Experience
Alpha diversity of biliary microbiota in bile from patients with pancreatic cancer vs cholangiocarcinoma.
Alpha diversity of biliary microbiota in bile from patients with pancreatic cancer vs cholangiocarcinoma.</p
Multivariate analysis and diversity of biliary microbiota in patients with pancreatic cancer versus cholangiocarcinoma using mixMC.
Multivariate analysis and diversity of biliary microbiota in patients with pancreatic cancer versus cholangiocarcinoma using mixMC.</p
Relative abundance of dominant phyla in bile from patients with pancreatic cancer and cholangiocarcinoma.
Relative abundance of dominant phyla in bile from patients with pancreatic cancer and cholangiocarcinoma.</p
Alpha diversity of biliary microbiota in patients with benign vs malignant pancreaticobiliary diseases.
Alpha diversity of biliary microbiota in patients with benign vs malignant pancreaticobiliary diseases.</p
Mann Whitney-U test showing differential abundance of bacterial genera in bile from patients with malignant (case) and benign (control) pancreaticobiliary diseases.
Mann Whitney-U test showing differential abundance of bacterial genera in bile from patients with malignant (case) and benign (control) pancreaticobiliary diseases.</p
Mann Whitney-U test showing differential abundance of bacterial genera in bile from patients with pancreatic cancer vs cholangiocarcinoma.
Mann Whitney-U test showing differential abundance of bacterial genera in bile from patients with pancreatic cancer vs cholangiocarcinoma.</p
Overall characteristics of all patients.
BackgroundThe prognostic and pathophysiologic significance of the biliary microbiota in pancreaticobiliary malignancies is little understood. Our goal was to find malignancy-related microbiomic fingerprints in bile samples taken from patients with benign and malignant pancreaticobiliary diseases.MethodsBile specimens were collected from consenting patients during routine endoscopic retrograde cholangiopancreatography. We used PowerViral RNA/DNA Isolation kit to extract DNA from bile specimens. The Illumina 16S Metagenomic Sequencing Library Preparation guide was used to amplify the bacterial 16S rRNA gene and create libraries. QIIME (Quantitative Insights Into Microbial Ecology), Bioconductor phyloseq, microbiomeSeq, and mixMC packages were used for post-sequencing analysis.ResultsOf 46 enrolled patients, 32 patients had pancreatic cancers, 6 had cholangiocarcinoma and 1 had gallbladder cancer. Rest of the patients had benign diseases including gallstones, and acute and chronic pancreatitis. We used multivariate approach in mixMC to classify Operational Taxonomic Units (OTUs). Doing this, we found a predominance of genera Dickeya (p = 0.00008), [Eubacterium] hallii group (p = 0.0004), Bacteroides (p = 0.0006), Faecalibacterium (p = 0.006), Escherichia-Shigella (p = 0.008), and Ruminococcus 1 (p = 0.008) in bile samples from pancreaticobiliary cancers as compared to benign diseases. Additionally, bile samples from patients with pancreatic cancer exhibited a predominance of genus Rothia (p = 0.008) as compared to those with cholangiocarcinoma, whereas bile samples from patients with cholangiocarcinoma exhibited a predominance of genera Akkermansia (p = 0.031) and Achromobacter (p = 0.031) as compared to those with pancreatic cancers.ConclusionsBoth benign and malignant pancreaticobiliary diseases have distinct microbiomic fingerprints. The relative abundance of OTUs in bile samples varies between patients with benign and malignant pancreaticobiliary diseases, as well as between cholangiocarcinoma and pancreatic cancer. Our data suggest that either these OTUs play a role in carcinogenesis or that benign disease-specific microenvironmental changes differ from cancer-specific microenvironmental changes, resulting to a clear separation of OTU clusters. We need more research to confirm and expand on our findings.</div
Multivariate analysis and diversity of biliary microbiota in patients with malignant (case) vs benign (control) pancreaticobiliary diseases using mixMC.
Multivariate analysis and diversity of biliary microbiota in patients with malignant (case) vs benign (control) pancreaticobiliary diseases using mixMC.</p